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QIAseq Immune Repertoire RNA Library Kits

For human and mouse immune repertoire screening
  • Unique Molecular Indices (UMI) ensure accurate sequencing results
  • Online data analysis through GeneGlobe
  • Includes QIAseq Beads for reaction cleanup
  • Automation-friendly protocol for human or mouse samples
  • QIAseq Sample Indices ordered separately to multiplex up to 384 samples


The QIAseq Immune Repertoire RNA Library Kits use Unique Molecular Indices (UMI) with gene-specific primers to target specific RNAs for NGS sequencing. Each unique panel is carefully designed and laboratory-verified for sequencing performance with a UMI-aware alignment software for maximum sequencing performance and accurate results. The Human and Mouse T-cell Receptors Panel is used for sequencing the V(D)J region of the alpha, beta, delta and gamma genes, including the CDR3 regions. Online analysis through the GeneGlobe Data Analysis Center provides key sequencing QC metrics, as well as the frequency and identity of each clonotype sequenced.
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QIAseq Immune Repertoire RNA Library Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


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Quick-Start Protocols (3)
Part 1: RT primer hybridization, reverse transcription, second strand synthesis, end-repair, A-addition

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Part 2: Adapter ligation, cleanup of adapter-ligated DNA, target enrichment
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Part 3: Cleanup of target enrichment, universal PCR, cleanup of universal PCR
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