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QIAamp 96 PowerFecal QIAcube HT Kit

For automated high-throughput purification of genomic DNA from fresh or frozen stool samples that are high in PCR inhibitors
  • Simple and reliable automated processing for cost and time savings
  • No hazardous organic chemicals required
  • Efficient removal of PCR inhibitors
  • High sensitivity in downstream assays
The QIAamp 96 PowerFecal QIAcube HT Kit provides simple, automated purification of high-quality genomic DNA (human and bacterial) from up to 200 mg fresh or frozen stool samples using the QIAcube HT. The novel combination of MOBIO's patented Inhibitor Removal Technology (IRT) and proven QIAamp purification system provides an optimized DNA purification solution for microbiome, infectious disease and metabolic research applications.
Cat No./ID: 19092
Pathogen Lysis Tubes L
1,900.00 kr
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50 Pathogen Lysis Tubes with large beads, 1 vial of Reagent DX
Cat No./ID: 19585
S-Blocks (24)
3,580.00 kr
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96-well blocks with 2.2 ml wells, 24 per case
Cat No./ID: 51531
QIAamp 96 PowerFecal QIAcube HT Kit (5)
15,445.00 kr
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For 480 preps: QIAamp 96 plates, QIAGEN Proteinase K, buffers
Cat No./ID: 950067
QIAcube HT Plasticware
2,735.00 kr
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Plasticware for 480 typical preps on QIAcube HT:
5 S-Blocks, 5 Elution Microtubes RS (EMTR), 120 x 8-Well Strip Caps for EMTR, 9 x 96 Filter-Tips OnCor C, TapePad
The QIAamp 96 PowerFecal QIAcube HT Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Comparable human DNA recovery with all kits whether used manually or automated.
Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR of β-Actin was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 2 µl eluate in a 25 µl reaction. Human DNA purified either using different instrument platforms or manually from all the three specimens showed similar performance in the assay.
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Comparable bacterial DNA recovery with all kits whether used manually or automated.
Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR of generic 16S rRNA gene sequence was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 1 µl eluate in a 20 µl reaction. DNA extracted either using different instrument platforms or manually from all the three specimens showed similar performance in the assay.
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Even for difficult-to-lyse gram-positive bacteria, all the kits yielded comparable DNA recovery whether used manually or automated.
Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR of 16S rRNA gene of Corynebacterium glutamicum was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 1 µl eluate in a 20 µl reaction. DNA extracted either using different instrument platforms or manually from all the three specimens showed similar performance in the assay.
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Efficient PCR inhibitor removal with all kits whether used manually or automated.
Aliquots of 200 mg of three different human stool specimens were processed on the QIAcube HT using the QIAamp 96 PowerFecal QIAcube HT Kit, on the QIAcube using the QIAamp DNA Stool Mini Kit and QIAamp Fast DNA Stool Mini Kit, or manually using the two DNA Stool kits. Real-time PCR was carried out on the Rotor-Gene Q using the QuantiTect Virus PCR Kit with 2 µl and 10 µl eluates respectively, from each sample in 20 µl reactions containing an internal control. CT values were compared to a standard containing no sample but only internal control (dark blue bar). All the three samples either extracted on different instruments or manually showed similar performance compared to the standard, regardless of the volume of eluate used, indicating no PCR inhibition.
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Automated bacterial DNA purification from various animal stool samples gave comparable yields to the manual procedure.
Aliquots of 200 mg stool sample from different animals were processed on the QIAcube HT instrument (blue bars) using the QIAamp 96 PowerFecal QIAcube HT Kit, or manually using the QIAamp Fast DNA Stool Mini Kit (pink bars). Real-time PCR of 16S rRNA gene of Corynebacterium glutamicum was carried out on the Rotor-Gene Q using the QuantiFast Probe PCR Kit with 1 µl eluate in a 20 µl reaction. DNA extracted either manually or on the QIAcube HT instrument showed similar performance for all the stool samples.
Performance
QIAcube HT and dedicated QIAcube HT purification kits let users increase sample purification throughput without having to compromise quality or reliability.

Effective removal of inhibitors
Stool samples are rich in PCR inhibitors such as complex polysaccharides, bile salts, lipids and urate. In the best cases, these inhibitors make it difficult to amplify targets by PCR, while in the worst cases, their presence can entirely suppress PCR signals. Therefore, effective removal of these inhibitors is critical to successful analysis of nucleic acids purified from stool samples.
Principle
The QIAamp 96 PowerFecal QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
Procedure
The QIAamp 96 PowerFecal QIAcube HT procedure is fast and simple with the QIAcube HT instrument. PCR inhibitors are removed by the MOBIO proprietary Inhibitor Removal Technology (IRT) with two precipitation steps. A combination of mechanical disruption and proteinase K digestion ensure complete lysis of even difficult-to-lyse organisms, resulting in high concentrations of unbiased DNA.

Buffering conditions are adjusted to provide optimal DNA binding, and the lysates are loaded onto the QIAamp 96 plate. As vacuum is applied, DNA binds to the QIAamp membrane, while contaminants pass through. Remaining impurities are removed in four efficient wash steps. Pure DNA is eluted under vacuum in a single step in 100 µl of Buffer ATE. No hazardous organic chemicals are used, increasing laboratory and personal safety.
Applications
The high-quality nucleic acids are ready to be used in a wide range of downstream applications, including PCR and quantitative real-time PCR, infectious disease and metabolic research applications.
Features
Specifications
Elution volume 100 μl
Sample amount Up to 200 mg fresh or frozen stool
Throughput 24-96 samples in increments of 8 samples
Time per run 96 samples in approximately 120 minutes 24 samples in approximately 60 minutes

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Quick-Start Protocols (1)
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
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Operating Software (1)
Files necessary for configuring the QIAcube HT Prep Manager Software to process QIAamp 96 PowerFecal QIAcube HT Kits. Not compatible with QIAcube HT Operating Software 4.17.

For kit configuration installation instructions, please refer to the QIAcube HT User Manual  chapter 4.4.5.
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Kit Handbooks (1)
For use with QIAcube HT Prep Manager Software; not compatible with QIAcube HT Operating Software 4.17
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