For the extraction of protein and nucleic acids from fungal cell cultures
Efficient solubilization of total nucleic acids and cellular proteins from fungal samples
Sequential DNA, RNA and protein isolation in less than 40 minutes without precipitation
Ready-to-use, high-quality nucleic acids and proteins for downstream applications
The AllPrep Fungal DNA/RNA/Protein Kit makes isolating proteins and nucleic acids easy. The user-friendly system incorporates both a bead beating method and silica spin columns to capture DNA, RNA and proteins in a streamlined process. DNA and RNA is ready for qPCR, RT-PCR and next-generation sequencing, while protein is ready for 1D SDS-PAGE and mass spectrometry following in-gel trypsin digestion. Some applications, including 2D SDS-PAGE and solution phase proteolytic digestion for mass spectrometry, may require an additional detergent removal step following protein elution.
Beginning with mechanical lysis of cultured fungal cells using 0.5 mm glass bead tubes, nucleic acids and proteins are solubilized and mixed with a binding solution. DNA is bound to the first spin column and the flow-through is combined with a solution to bind the total RNA on a second spin column. The final flow-through captures protein. Each of the individual spin columns is then washed and the immobilized analyte is eluted.
The AllPrep Fungal DNA/RNA/Protein Kit was previously sold by MO BIO under the same name.
For the extraction of protein and nucleic acids from fungal cell cultures
The Allprep Fungal DNA/RNA/Protein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Features
Specifications
Bead size
0.5 mm glass
Format
Silica Spin Filter
Processing
Chemical lysis and/or bead beating
Sample size
1.8 ml of microbial culture
QIAGEN recommends using no more than 1x10^9 bacterial cells per sample
Sample types
Cultured fungi
Storage temperature
Store spin filters at 4°C.
All other kit reagents and components can be stored at room temperature (15-30°C).
Buffers and reagents for DNA fragmentation (including end repair and A-addition), ligation and library amplification; for use with Illumina instruments; includes a plate containing 96 adapters with different barcodes (pierceable foil seal, allowing usage of defined parts of plate)
