High-quality genomic DNA purified from 48 blood samples using the BioRobot M48 and the MagAttract DNA Blood M48 Kit. Eluate (10 µl of each 200 µl) was analyzed by agarose gel electrophoresis.
Amplification of a single-copy gene (MECL-1) using template DNA purified from whole blood. Samples 1–6 and 43–48 are from the first and last sample prep plate positions on the BioRobot M48 worktable, respectively. All blood samples were obtained from the same donor. M: 100 bp DNA ladder (100 ng); +: positive control; –: negative control. A 5 µl aliquot (10%) of each PCR was loaded on the agarose gel.
Amplification (34 cycles) of the MECL-1 single-copy gene (900 bp fragment) from a dilution series of DNA purified from 200 µl ACD-, heparin-, and EDTA-preserved whole blood samples. M: 100 bp DNA ladder (100 ng); C-: negative control; C+: positive control.