QIAamp DNA Investigator Kit

For purification of DNA from forensic and human identity samples

Features

  • Rapid purification of high-quality, ready-to-use DNA
  • Consistent, high yields
  • Complete removal of contaminants and inhibitors
QIAamp DNA Investigator Kit (50)

Cat. No. / ID: 56504

For 50 DNA preps: 50 QIAamp MinElute Columns, Proteinase K, Carrier RNA, Buffers, Collection Tubes (2 ml)
SEK 3,085.00
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The QIAamp DNA Investigator Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

Product Details

The QIAamp DNA Investigator Kit provides purification of genomic DNA from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. The kit uses QIAamp MinElute spin columns for purification of high-quality DNA with flexible elution volumes. Purification is fast and efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios. Purification of DNA using the QIAamp DNA Investigator Kit can be automated on the QIAcube Connect.

Performance

The QIAamp DNA Investigator Kit is suited for DNA purification from trace samples (e.g., from crime scene); genotyping, including fingerprinting and paternity analysis; and routine analysis of reference samples.

After sample lysis, the simple QIAamp DNA Investigator procedure, which is highly suited for simultaneous processing of multiple samples, yields pure DNA in less than 30 minutes.  DNA is eluted in Buffer ATE or water, and is immediately ready for use in amplification reactions or for storage at –20°C. The purified DNA is free of proteins, nucleases, and other inhibitors.

Principle

The QIAamp DNA Investigator Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 100 μl.

Procedure

Using QIAamp MinElute spin columns for purification of high-quality quality DNA with flexible elution volumes, the QIAamp DNA Investigator procedure consists of 4 steps (see flowchart " Procedure"). The sample is lysed under denaturing conditions with proteinase K. DNA binds to the membrane, contaminants flow through, and residual contaminants are washed away. Pure, concentrated DNA is eluted from the membrane. The QIAamp DNA Investigator Kit is automatable on the  QIAcube

The procedure is designed to ensure that there is no sample-to-sample cross-contamination.

See figures

Applications

The QIAamp DNA Investigator Kit uses well-established technology for purification of genomic and mitochondrial DNA from small sample volumes or sizes.

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsReal-time PCR, STR analysis
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinGenomic DNA, mitochondrial DNA
Sample typeswide range of forensic and human-identity sample materials
ProcessingManual
Elution volume20-100 µl
Time per run or per prepless than 30 minutes
TechnologySilica technology
FormatSpin column
Yield< 3 µg

Resources

Kit Handbooks (1)
Supplementary Protocols (2)
Supplementary protocol using the EZ1 Advanced or EZ1 Advanced XL with the EZ1 DNA Investigator Kit
Supplementary protocol using the Investigator Lyse&Spin Basket Kit and EZ1 Advanced XL
Technical Information (1)

FAQ

I received a kit containing the MinElute columns; however, they were left out for a while and not stored at 2–8°C upon receipt. Can I still use them?

The MinElute spin columns included in the following kits should be stored at 2–8°C upon arrival: AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro.

Short-term storage (up to 4 weeks) at room temperature (15–25°C) does not affect the performance. However, for optimal performance and quality, storage temperature should not exceed 25°C.

FAQ ID - 3560
What is the composition of elution buffer ATE in the QIAamp DNA Investigator kit, QIAamp DNA FFPE Tissue kit and the QIAamp Fast DNA Stool Mini kit?

The composition of Buffer ATE is:

- 10 mM Tris-Cl pH 8.3

- 0.1 mM EDTA

- 0.04% NaN3 (sodium-azide)

FAQ ID -3122
Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
What can be used as an alternative to the A260 measurement for quantification of small amounts of RNA and DNA?

Small amounts of RNA and DNA may be difficult to measure spectrophotometrically. Fluorometric measurements, or quantitative RT-PCR and PCR are more sensitive and accurate methods to quantify low amounts of RNA or DNA.

Fluorometric measurements are carried out using nucleic acid binding dyes, such as RiboGreen® RNA Quantitation Reagent for RNA, and PicoGreen® DNA Quantitation Reagent for DNA (Molecular Probes, Inc.).

FAQ ID -728