Ni-NTA HRP Conjugate
For simple, direct detection of His-tagged proteins without secondary antibodies
Ni-NTA HRP Conjugate is used to detect recombinant proteins carrying His tags without the need for antibodies. The Ni-NTA moiety binds to a protein's His tag and the complex is localized using chromogenic substrates that react with conjugated horseradish peroxidase (HRP). Buy Products
The Ni-NTA HRP Conjugate is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Product Details
Performance
Principle
Ni-NTA HRP Conjugate consists of Ni-NTA coupled to horseradish peroxidase (HRP). It can be used for direct, straightforward detection of any recombinant protein with an accessible His tag. It eliminates the need for secondary antibodies, thereby saving time in detection procedures. Ni-NTA HRP Conjugate is recommended for use with chromogenic substrates, and for E. coli expression systems. The use of chemiluminescent substrates with Ni-NTA conjugates is not recommended.
Applications
Ni-NTA HRP Conjugates are recommended for detection of proteins in E.coli by western, dot, and colony blotting procedures.
Specifications
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