PAXgene Blood miRNA Kit
For purification of miRNA and total RNA from whole blood
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PAXgene Blood miRNA Kit (50)
Cat. No. / ID: 763134
For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers; to be used in conjunction with PAXgene Blood RNA Tubes
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Features
- Integrated system for collection, stabilization, and purification
- Effective purification of miRNA and total RNA
- RNA stabilization for up to 3 days at 18–25°C
- Stabilization for at least 50 months at –20°C or –70°C
Product Details
The PAXgene Blood miRNA System consists of the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides, and PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for collection, stabilization, and transport of blood. Purification is carried out using silica-based RNA purification technology in a spin-column format. Purification can be carried out manually, using a microcentrifuge, or automated on the QIAcube Connect. Used with the tubes, the kit provides a complete preanalytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis.
Performance
Blood samples collected in PAXgene Blood RNA Tubes can be safely stored or transported at 15–25°C for up to 72 hours, at 2–8°C for up to 5 days, or at –20°C or –70°C for at least 50 months without showing any significant RNA degradation or changes in transcript levels. Total RNA >18 nucleotides is purified from the stabilized blood samples with the PAXgene Blood miRNA Kit and includes both mRNA and small RNAs such as miRNA (see figure " High yields of small RNA species"). The miRNA species can be analyzed using the QIAGEN miScript PCR System (see figure " Analysis of miRNA species") or other real-time RT-PCR systems.
See figures
Principle
Blood samples are collected in PAXgene Blood RNA Tubes. The tubes contain a reagent that lyses blood cells and immediately stabilizes intracellular RNA to preserve the gene expression profile. RNA stabilization is critical for reliable downstream gene expression analysis. Without stabilization, degradation of RNA and upregulation or downregulation of transcripts occur immediately after blood is drawn.
Procedure
With the PAXgene Blood miRNA Kit, total RNA >18 nucleotides (including miRNA) is purified from the stabilized blood samples using well-established PAXgene silica-membrane technology. Purification can be carried out in a manual format or it is automatable on the QIAcube (see figure " The QIAcube").
See figures
Applications
The purified total RNA is ready to use and is ideally suited for any downstream application, including:
- RT-PCR and real-time RT-PCR
- Differential display
- cDNA synthesis
- Northern, dot, and slot blot analyses
- Primer extension
- Poly A+ RNA selection
- RNase/S1 nuclease protection
- Microarray analysis
Supporting data and figures
Analysis of miRNA species.
Blood was collected in PAXgene Blood RNA Tubes and stored for 20–24 hours at room temperature before freezing at –15 to–30°C. RNA was purified using the PAXgene Blood RNA Kit or the PAXgene Blood miRNA Kit, as indicated. miRNA species were analyzed using the QIAGEN miScript PCR System, with the indicated miScript Primer Assays. The lower CT values for RNA purified using the PAXgene Blood miRNA Kit indicate higher amounts of those miRNA species.
Specifications
| Features | Specifications |
| Applications | RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, northern, dot, and slot blot analyses, primer extension, microarray analysis |
| Elution volume | 80 µl |
| Time per run or per prep | Manual: 90 min/12 samples; Automated: 125 min/12 samples |
| Technology | Silica technology |
| Main sample type | Whole blood |
| Yield | 3–18 µg/2.5 ml sample |
| Format | Spin column |
| Processing | Manual (centrifugation) or on QIAcube |
| Sample amount | 2.5 ml |
Resources
Brochures & Guides (3)
Safety Data Sheets (1)
Scientific Posters (3)
Application Notes (2)
Kit Handbooks (1)
FAQ
Where can I find additional information for PreAnalytiX PAXgene products?
Can the PAXgene Blood miRNA System be used for diagnostic or prognostic procedures?
What are the expected yields of RNA from PAXgene Blood RNA Tubes isolated with the PAXgene Blood miRNA kit?
Is it possible to isolate genomic DNA or viral nucleic acids with PAXgene Blood miRNA protocol?
What can I use to isolate RNA smaller than 200 nucleotides?
What is the maximum number of samples for the QIAcube variant of the PAXgene Blood miRNA protocol for a single run?
How great is the enrichment of miRNA when using the dedicated PAXgene Blood miRNA Kit compared to the PAXgene Blood RNA Kit?
Do eluates generated with the PAXgene Blood miRNA Kit contain residual genomic DNA?
Which sample types can be processed using the PAXgene Blood miRNA protocols?
Do you have stability data for RNA isolated with PAXgene miRNA Kit?
How many samples can be processed with the PAXgene Blood miRNA Kit?
Do the samples require pretreatment before starting the manual protocol or before loading onto the QIAcube instrument?