PAXgene Blood miRNA Kit
For purification of miRNA and total RNA from whole blood
For purification of miRNA and total RNA from whole blood
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Cat. No. / ID: 763134
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
The PAXgene Blood miRNA System consists of the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides, and PAXgene Blood RNA Tubes (available from BD, cat. no. 762165) for collection, stabilization, and transport of blood. Purification is carried out using silica-based RNA purification technology in a spin-column format. Purification can be carried out manually, using a microcentrifuge, or automated on the QIAcube Connect. Used with the tubes, the kit provides a complete preanalytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis.
Blood samples are collected in PAXgene Blood RNA Tubes. The tubes contain a reagent that lyses blood cells and immediately stabilizes intracellular RNA to preserve the gene expression profile. RNA stabilization is critical for reliable downstream gene expression analysis. Without stabilization, degradation of RNA and upregulation or downregulation of transcripts occur immediately after blood is drawn.
The purified total RNA is ready to use and is ideally suited for any downstream application, including:
Features | Specifications |
Applications | RT-PCR and real-time RT-PCR, differential display, cDNA synthesis, northern, dot, and slot blot analyses, primer extension, microarray analysis |
Elution volume | 80 µl |
Time per run or per prep | Manual: 90 min/12 samples; Automated: 125 min/12 samples |
Technology | Silica technology |
Main sample type | Whole blood |
Yield | 3–18 µg/2.5 ml sample |
Format | Spin column |
Processing | Manual (centrifugation) or on QIAcube |
Sample amount | 2.5 ml |