QIAxcel DNA Kits

For automated analysis of DNA fragments using QIAxcel instruments

S_1084_5_GEN_V2

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QIAxcel DNA High Resolution Kit (1200)

Cat. No. / ID:  929002

QIAxcel DNA High Resolution Gel Cartridge, Buffers, Mineral Oil, QX Intensity Calibration Marker, 12-Tube Strips
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SEK 11,695.00
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Cartridge type
High Resolution
Screening
Fast Analysis
High Sensitivity
QIAxcel DNA Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • Safety and convenience with ready-to-use gel cartridges
  • Streamlined workflow minimizes analysis time
  • Robust results for nucleic acid concentrations as low as 5 pg/µl
  • Accurate analyses with resolution down to 3–5 bp
  • Standardized processing with digital data collection

Product Details

QIAxcel instruments, when used in conjunction with QIAxcel DNA Kits, provide fully automated size separation and quantification of DNA fragments in up to 96 samples per run. QIAxcel technology, based on capillary electrophoresis using gel cartridges, provides unmatched resolution, speed, and throughput. QIAxcel gel cartridges are reusable, allowing up to 250 runs of 12 samples to be performed. Preinstalled methods suitable for most applications are provided. QIAGEN can also create customized methods for users.

The QIAxcel DNA High Sensitivity Kit only works with the QIAxcel Connect. All other QIAxcel DNA Kits work with either the QIAxcel Connect or QIAxcel Advanced Instruments. QIAxcel DNA Accessories are compatible with all QIAxcel DNA Kits.

Performance

Easy high-resolution analysis

Genotyping organisms, PCR-based cDNA library screening, and many other biomedical research and molecular biology applications require discrimination of PCR fragments that vary in size by a small number of base pairs. The QIAxcel High Resolution Kit, in combination with QIAxcel instruments, allows analysis of DNA fragments between 15 bp and 5 kb in size. Fragments smaller than 500 bp can be separated with a resolution of 3–5 bp, providing greater confidence in data interpretation than is possible with conventional high-resolution agarose gel electrophoresis (see  Sensitive detection of a mutated cancer-related gene). At a detection sensitivity of 0.1 ng/µl DNA in undiluted amplification reactions, QIAxcel instruments offer more robust results with less sample input material.

The QIAxcel DNA Screening Kit is highly suited for routine evaluation of fragments for restriction endonuclease digestion, qualitative singleplex or multiplex PCR, and other DNA applications (see  High-resolution analysis of multiplex PCR samples). The use of gel cartridges for DNA screening streamlines the lab workflow by minimizing analysis time for fragments of 15 bp to 5 kb in size - 96 samples are processed in approximately 1 hour. A resolution of 20–50 bp can be obtained for fragments smaller than 1 kb. Sample consumption is less than 0.1 µl, leaving virtually all of your precious sample for further downstream use.

The QIAxcel DNA Fast Analysis Kit is highly suited for routine evaluation of DNA fragments in qualitative single or multiplex PCR applications. Use of gel cartridges for DNA screening streamlines the lab workflow by minimizing analysis time for fragments of 15 bp to 3 kb in size — 12 samples are processed in just 3 minutes and 96 samples in approximately 25 minutes (see  Screenshot showing data from PCR fragment analysis). A resolution of down to 50 bp can be achieved with the QIAxcel DNA Fast Analysis gel cartridge, while sample consumption is less than 0.1 μl.

The QIAxcel DNA High Sensitivity Kit lets you analyze DNA in concentrations as low as 5 pg/μl. Analysis time for fragments 15 bp – 3 kb in size is around 9 minutes for 12 samples. A resolution of 20–50 bp can be achieved with the QIAxcel DNA High Sensitivity gel cartridge.

See figures

Principle

QIAxcel instruments use capillary gel electrophoresis to enable fast size-based separation of nucleic acids (see  Sample separation process). Unlike traditional agarose gel electrophoresis, separation is performed in a capillary of a ready-to-use gel cartridge. The samples are automatically loaded into an individual capillary and voltage is applied. The negatively charged nucleic acid molecules migrate through the capillary to the positively charged terminus. As with agarose gel electrophoresis, low-molecular-weight molecules migrate faster than high-molecular-weight molecules. As the molecules migrate through the capillary, they pass a detector which detects and measures the fluorescent signal. A photomultiplier converts the fluorescent signal into electronic data, which are then transferred to the computer for further processing using QIAxcel ScreenGel software. After processing, the data are displayed as an electropherogram or gel image.

See figures

Procedure

QIAxcel DNA Kits simplify separation and analysis of DNA fragments, and provide increased user safety. Just a few simple steps are required: load the gel cartridge, fill and load the buffer tray, load the samples in 96-well plates or in PCR tubes or strips, select the process profile to be used, and go. Tedious gel preparation and extensive user training are eliminated, streamlining the workflow and facilitating integration of the system into the daily lab routine. Within minutes of starting a run, the first results appear in real time on the computer screen (see  Reduced run times).

See figures

Applications

Preprogrammed methods, in combination with the corresponding gel cartridges, allow separation and analysis of a variety of nucleic acids, including single or multiple PCR fragments, DNA digested with restriction endonucleases, and synthesized oligonucleotides.

  QIAxcel DNA High Resolution Kit QIAxcel DNA High Sensitivity Kit§ QIAxcel DNA Screening Kit QIAxcel DNA Fast Analysis Kit
Size range 15 bp – 20 kb; displaying up to 50 kb 15 bp – 3 kb 15 bp – 5 kb 15 bp – 3 kb
Resolution* 3–5 bp 20–50 bp 20–50 bp 50 bp
Limit of detection 0.1 ng/μl 5 pg/μl 0.1 ng/μl 0.1 ng/μl
Samples per cartridge 1200 samples 1200 samples 2400 samples 3000 samples
Run time 9–25 min ~9 min ~7 min ~3 min
Application area
  • High-resolution genotyping
  • VNTR, STRs, etc.
  • gDNA integrity check
  • NGS library check
  • Quality control of liquid biopsy extracts
  • NGS library check
  • Fast PCR screening (single and multiplex)
  • Restriction digest, plasmid
  • gDNA integrity check
  • NGS library check
  • Fast single amplicon analysis, e.g., HLA typing

Supporting data and figures

Resources

Brochures & Guides (7)
A protocol for evaluation of samples for next-generation sequencing on the QIAGEN GeneReader platform using the QIAxcel Advanced Instrument and ScreenGel software version 1.5 of higher
For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader platform using ScreenGel Software version 1.5 or higher
For evaluation of samples for next-generation sequencing (NGS) on the QIAGEN GeneReader instrument

For analysis and typing of PCR products from different HLA loci using the QIAxcel Advanced and the Helmberg-SCORE software

Kit Handbooks (2)
For automated electrophoretic analysis of DNA using the QIAxcel Connect instrument

June 2022
For automated analysis of DNA fragments using the QIAxcel and QIAxcel Advanced instruments
Application Notes (20)
The QIAxcel System was successfully used to detect DNA derived from genetically modified organisms (GMOs) at a level suitable for GMO testing according to EU standards.
Operating Software (2)
This release includes the first version of QIAxcel ScreenGel NGS Profiles. These profiles are intended to be used to run and analyze reactions of GeneRead QIAact AIT and BRCA 1/2 panels using the QIAxcel instrument and QIAxcel ScreenGel 1.5 Software. These profiles are composed of an Process Profile, Analysis Profile, Distribution Profile, and Report/Export Profile.
Quick-Start Protocols (1)
For electrophoretic analysis of DNA samples using the QIAxcel Connect system

June 2022
Instrument User Manuals (2)
For use with QIAxcel ScreenGel software v2.0
For use with QIAxcel Advanced instruments and QIAxcel ScreenGel Software version 1.6
Certificates of Analysis (1)

FAQ

Why does an intensity calibration of the gel cartridge for the QIAxcel System and QIAxcel Advanced need to be performed?

The intensity calibration procedure on the QIAxcel System and QIAxcel Advanced is performed to normalize the signal intensities across all 12 channels of the gel cartridge. This corrects for natural intensity reading variations between each capillary in the cartridge. A correction factor is determined and applied for every subsequent run performed with the cartridge.

FAQ ID -1824
Can a QIAxcel DNA Fast Analysis Cartridge be run on a QIAxcel System?

The QIAxcel DNA Fast Analysis Cartridge can only be run on the QIAxcel Fast Analysis System with the BioCalculator Fast Analysis software and the grey software key.

All other QIAxcel Cartridges can only be run on the QIAxcel System with the BioCalculator software and the blue software key.

FAQ ID -1828
Can customers use their own DNA size marker with the QIAxcel System and if yes, what concentration should be used?

The concentration of the DNA marker, pUC18/HaeIII, provided in the QIAxcel DNA Kits is 5ug/ml in 1XTE buffer solution. Customers can run their own DNA size marker sample with different concentration levels using the provided Methods (i.e. M500, H500, L500 and so on).

The recommended method for all basic applications with the QIAxcel System is the M500 method. The BioCalculator software also allows customers to choose their own Sample Injection Time in the Sequence table. The M500 method provides 5KV for 20 seconds for the electro-kinetic injection of lower concentrated DNA samples. If the Injection Time is doubled or tripled (40 or 60 sec), you can expect double or triple intensities (also peak heights).

FAQ ID -1841
Where are the QIAxcel data stored?

For the BioCalculator software, the default data folder is:

C:\Program Files\QIAGEN\QIAxcel BioCalculator\Data.

For the ScreenGel software, the default data folders are:

C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Experiment (for a computer with Windows XP operation system) or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Experiment\ (for a computer with Windows 7 operating system).

Alternatively, you can more conveniently access the ScreenGel experiment data folder using the menu options File\Open data directory\Experiment within the ScreenGel software.

FAQ -9008
If a different DNA size and/or alignment marker is to be used with the QIAxcel System or QIAxcel Advanced, does the Gel Cartridge need to be recalibrated?

The Gel Cartridge does not need to be recalibrated when using a new alignment marker and/or DNA size marker with the QIAxcel System or QIAxcel Advanced. Calibration is required only once, i.e. when the first cartridge is run or when the cartridge is used on a different QIAxcel system than the one it has been calibrated on, or when using a different PC than the one used to calibrate initially.

FAQ ID -1858
How can drying out of the gel cartridge tips used on the QIAxcel System and QIAxcel Advanced be prevented?

If the gel cartridge tips for the QIAxcel System and QIAxcel Advanced are in contact with air, the tips will dry out. There are 3 ways of preventing the tips from drying.

  1. Store the cartridge in the instrument with the instrument in the “Park” position. The washing solution (8 ml) covered with mineral oil must be in the “Park” position of the Solution Tray. 
  2. Store the cartridge in the Cartridge Stand with enough mineral oil in the well of the Cartridge Stand to cover the capillary tips. The Cartridge Stand should only be used for short term storage, i.e. when changing cartridges during one working day. 
  3. Place the cartridge back into its packaging with the capillary tips gently inserted into the soft gel in the box.
FAQ ID -1823
Why does the ScreenGel software version 1.2 crash when I try to use an old reference size marker table?

The reference size marker table created by ScreenGel version 1.1 or earlier is incompatible with ScreenGel version 1.2. Re-analyze the size marker run in ScreenGel version 1.2 and create a new reference size marker table for future use.

FAQ -9009
Why is the cartridge not recognized?

When using the BioCalculator software, ensure the color of the software key matches the type of cartridge used. For example, the QIAxcel Fast Analysis cartridge requires the red software key, while the QIAxcel High Resolution cartridge requires the blue software key.

In addition, check that the communication between the computer and the instrument is established. Further, check that the smart key is inserted securely.

If the issue persists, contact QIAGEN Technical Services and provide the cartridge ID and lot number, which is printed on the packaging of QIAxcel cartridges.

FAQ -9004
How do I perform the filter test?

When using the BioCalculator software, select Settings under File in the Instrument control panel window. In the new windows, click Check Filter in the new window and follow the on-screen prompts.

When using the ScreenGel software, log in to the ScreenGel software with an account with basic or advanced privilege.  Select System Check under the Service environment. Select Filter Check and click Start.

Please send the filter test result to QIAGEN Technical Services for further assistance.

FAQ -9006
Why is the signal of the QX alignment marker weak?

Incorrect storage can lead to QX alignment marker degradation. The QX alignment markers can be used for 50 runs or 3 days, whichever comes first. Opened QX alignment marker should be store at –20°C between uses.

FAQ -9003
What causes the calibration to fail?

Clogged capillaries and/or re-using the calibration marker are the most common causes of failed calibration. Perform “Removing gel from blocked capillaries”, as outlined in Appendix D in the QIAxcel DNA handbook. Use a new set of calibration marker for each new calibration procedure.

FAQ -9001
I have upgraded the ScreenGel software to version 1.2. How can I stop the software crash when I try to select a cartridge?

Please delete the 6 obsolete method files as outlines in the Important Note QIAxcel ScreenGel Software

FAQ -9010
How do I perform the detector test?

When using the BioCalculator software, select Detector test under File in the Instrument control panel window. Perform the Detector test with and without a cartridge latched inside the QIAxcel. Feedback is not shown on the screen when you select the Detect test. Instead, the file named detector.asc is saved at C:\Program Files\QIAGEN\QIAxcel BioCalculator. Change the file name before performing another detector test.

Note: Older versions of BioCalculator software save the detector.asc at C:\Program Files\QIAxcel BioCalculator.

When using the ScreenGel software, log in to the ScreenGel with an account with basic or advanced privilege.  Select System Check under the Service environment. Select Detector Test and click Start. The file is saved at C:\Documents and Settings\All Users\Application Data\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting\Troubleshooting_xxxxxxxx (current date in the format of YYYYMMDD) for a computer with Windows XP operating system or C:\ProgramData\QIAGEN\QIAxcel\ScreenGel\Data\Troubleshooting for a computer with Windows 7 operating system. Alternatively, you can perform a screen capture for the Detector test result.

Please send the Detector test results to QIAGEN Technical Services for further assistance.

FAQ -9006