QIAwave DNA Blood & Tissue Kit

For a more eco-friendly alternative to our standard kit for extracting total DNA from animal blood and tissues, cells, yeast, or bacteria.

S_1220_6_LS_QF_QIAwave_DNA_Blood_Tissue

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QIAwave DNA Blood & Tissue Kit (250)

Cat. No. / ID: 69556

250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Waste Tubes (2 ml).
SEK 10,070.00
KitKit component
QIAwave DNA Blood & Tissue Kit
Waste Tubes
The QIAwave DNA Blood & Tissue Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Features

  • DNA quality and performance identical to the DNeasy Blood & Tissue Kit
  • 63% less plastic and 42% less cardboard compared to the DNeasy Blood & Tissue Kit
  • Reusable Waste Tubes made from 100% post-consumer recycled plastic
  • Buffer concentrates that use 74–80% less plastic than our standard buffers

Product Details

The QIAwave DNA Blood & Tissue Kit is an eco-friendlier version of our standard DNeasy Blood & Tissue Kit. The QIAwave Kit uses 63% less plastic and 42% less cardboard than our standard kit and waste tubes made from 100% post-consumer recycled plastic that you can reuse throughout the procedure. QIAwave buffers also come as concentrates, reducing the amount of plastic by 74–80% per bottle. To save paper, there are no printed protocols in the kit. You can download the protocols you need from the Resources section below or by scanning the QR code inside the kit box. So, while the packaging and components of our QIAwave Kit may look different, it’s as easy to use the QIAwave DNA Blood & Tissue Kit and the chemistry and performance are identical.

In partnership with My Green Lab, we've also assessed the environmental impact of this kit. My Green Lab ACT (accountability, consistency, and transparency) environmental impact factor labels are designed to evaluate and score products on several sustainability criteria, including:

  • manufacturing
  • responsible chemical management
  • sustainable content within products and packaging materials
  • disposal of the packaging at the end of life

Products are scored from 1 to 10 except for energy and water consumption, which are scored as 1 point per kWh or gallon, respectively. A low score means a lower environmental impact. You can view the ACT labels for this kit in the Resources section below.

The QIAwave Kit uses silica-based spin-columns for purification, and most samples can be lysed directly with proteinase K. This means:

  • No mechanical disruption
  • No organic extraction
  • No alcohol precipitation

The standard protocol for this kit allows you to extract total DNA from animal blood and tissue samples. We’ve also developed protocols for other samples types to ensure you get reproducible quantities of high-quality DNA, whether you’re working in life science, genotyping or veterinary pathogen research. You can also automate your sample extraction on the QIAcube Connect.

Performance

The performance between the QIAwave DNA Blood & Tissue Kit and the DNeasy Blood & Tissue Kit is identical because the chemistry is the same. We’ve also shown that both kits outperform competitors’ kits (see figure “ QIAwave DNA Blood & Tissue Kit performance”).
The standard protocol of the QIAwave DNA Blood & Tissue Kit gives high yields of total DNA from animal blood and tissue samples (see table "Typical DNA yields from animal tissues using QIAwave DNA Blood & Tissue " and figure  "DNA yields"). However, we also offer optimized protocols to ensure high yields from nonstandard samples, such as:

  • Animal hair
  • Cultured cells
  • Gram-positive and -negative bacteria
  • Yeast
  • Insects
  • Other sample types

Typical yields from animal tissues using QIAwave DNA Blood & Tissue Kit

Source Amount DNA(µg)
Mammalian blood 100 µl 3–6
Bird blood 5 µl 9–40
HeLa cells 2 x 106 15–25
Liver 25 mg 10–30
Brain 25 mg 15–30
Kidney 25 mg 15–30
Spleen 10 mg 5–30
Mouse tail 1.2 cm (tip) 10–25
Rat tail 0.6 cm (tip) 20–40
Pig ear 25 mg 10–30
Horsehair 10 hairs 2–4
Fish fin 20 mg 10–20
Fish spawn (mackerel) 10 mg 5–10
See figures

Principle

The QIAwave DNA Blood & Tissue Kit rapidly purifies total DNA (e.g., genomic, mitochondrial, and pathogen) from different sample types, including fresh or frozen animal tissues and cells, blood or bacteria.
The membrane combines the binding properties of a silica-based membrane with simple microspin technology. The DNA adsorbs to the membrane in the presence of high concentrations of chaotropic salt, which removes water from hydrated molecules in solution. The buffer conditions allow specific adsorption of DNA to the silica membrane and removal of contaminants and enzyme inhibitors.
The entire purification process does not require phenol or chloroform extraction or alcohol precipitation and involves minimal handling, which means you can easily process multiple samples simultaneously.

Procedure

The QIAwave DNA Blood & Tissue Kit purifies DNA using DNeasy Mini Spin-Columns containing a silica-membrane. This makes the procedure fast and reproducible while eliminating manual and organic extraction or alcohol precipitatoin (see flowchart " QIAwave DNA Blood & Tissue Kit procedure").

Four simple steps to purify high-quality DNA:

  1. Lyse samples in proteinase K. The buffering conditions provide optimal DNA-binding conditions.
  2. Load the lysate onto the DNeasy Mini Spin Column.
  3. Centrifugate samples. The DNA is selectively bound to the membrane as contaminants pass through.
  4. Remaining contaminants and enzyme inhibitors are then removed in two efficient wash steps, and DNA is then eluted in water or buffer, ready for you to use.

QIAwave buffers come as concentrates that you can easily reconstitute by adding water and/or ethanol; please check the handbook for details. QIAwave DNeasy Mini Spin Columns and Waste Tubes come in individual bags and need to be preassembled before you start the protocol. This takes a little extra time, but it does reduce plastic waste.
If you have too many samples and not enough time to process them manually, you can automate the purification process using the DNeasy Mini Spin Columns on the QIAcube Connect.

See figures

Applications

QIAwave DNA Blood & Tissue provides high-quality DNA, ready to use in all downstream assays, including applications in:

  • Life science research
  • Livestock breeding
  • Pedigree genotyping
  • Veterinary pathogen research
  • Routine applied testing

Supporting data and figures

Specifications

FeaturesSpecifications
ApplicationsNGS, PCR, real-time PCR, genotyping
Elution volume100–200 µl
Time per run or per prep20 minutes
Main sample typeBlood, tissue
FormatSpin column
Sample amount100 µl blood / 25 mg tissue / 5 x 106 cultured cells
ProcessingManual or QIAcube Connect
Yield5 - 30 µg
TechnologySilica technology
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinDNA

FAQ

Do you have a protocol for purification of total DNA from crude lysates?

Yes, please follow the Supplementary Protocol 'Purification of total DNA from crude lysates using the DNeasy Blood & Tissue Kit' (DY15).

 

FAQ ID -1255
Do you have a protocol for isolation of genomic DNA from saliva and mouthwash?

Yes, we have the following protocols:

  • Isolation of genomic DNA from saliva and mouthwash using the QIAamp DNA Mini Kit; vacuum procedure (QA19v)
  • Isolation of genomic DNA from saliva and mouthwash using the QIAamp DNA Mini Kit; spin procedure (QA19s)
  • Isolation of genomic DNA from saliva using the DNeasy Blood & Tissue Kit; spin procedure (DY07)
FAQ ID -917
Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699
Do you have a protocol for purification of total DNA from yeast?

Yes, please follow the Supplementary Protocol 'Purification of total DNA from yeast using the DNeasy Blood & Tissue Kit' (DY13).

 

FAQ ID -1253
Do you have a protocol for the isolation of DNA from soft tissues using the TissueLyser?
Do you have a protocol for purification of total DNA from insects?

Yes, please follow the Supplementary Protocol 'Purification of total DNA from insects using the DNeasy Blood & Tissue Kit' (DY14).

 

FAQ ID -1254
How can I precipitate genomic DNA using isopropanol?

Alcohol precipitation is commonly used for concentrating, desalting, and recovering nucleic acids. Since less alcohol is required for isopropanol precipitation, this is the preferred method for precipitation of DNA from large volumes. In addition, isopropanol precipitation can be performed at room temperature, which minimizes co-precipitation of salt that interferes with downstream applications.

 

Procedure

  1. Adjust the salt concentration, for example, with sodium acetate (0.3 M, pH 5.2, final concentration) or ammonium acetate (2.0–2.5 M, final concentration).
  2. Add 0.6–0.7 volumes of room-temperature isopropanol to the DNA solution and mix well.
  3. Centrifuge the sample immediately at 10,000–15,000 x g for 15–30 min at 4°C
  4. Carefully decant the supernatant without disturbing the pellet.
  5. Wash the DNA pellet by adding 1–10 ml (depending on the size of the preparation) of room-temperature 70% ethanol. This removes co-precipitated salt and replaces the isopropanol with the more volatile ethanol, making the DNA easier to redissolve.
  6. Centrifuge at 10,000–15,000 x g for 5–15 min at 4°C.
  7. Carefully decant the supernatant without disturbing the pellet.
  8. Air-dry the pellet for 5–20 min (depending on the size of the pellet).
  9. Redissolve the DNA in a suitable buffer.

Tip: Use a buffer with a pH of 7.5–8.0, as DNA does not dissolve easily in acidic buffers. Often distilled water can have an acidic pH. The addition of EDTA protects the DNA from DNase digestion.

Tip: High-molecular-weight DNA, such as genomic DNA, should be redissolved very gently to avoid shearing. If the DNA pellet does not dissolve easily, heat at 55°C for 1–2 h with gentle shaking.

FAQ ID -2953
What is the shelf-life for QIAGEN Proteinase K (cat. no. 19131, 19133)?

QIAGEN Proteinase K is stable for up to 1 year after delivery when stored at room temperature. To prolong the shelf-life of Proteinase K, storage at 2–8°C is recommended.

FAQ ID - 3447
Do you have a protocol for the isolation of genomic DNA from sperm?

Yes, we have the following protocols:

  • Isolation of genomic DNA from sperm using the QIAamp DNA Mini Kit; protocol 1 (QA03), long procedure
  • Isolation of genomic DNA from sperm using the QIAamp DNA Mini Kit; protocol 2 (QA04), short procedure
  • Purification of total DNA from animal sperm using the DNeasy Blood and Tissue kit; protocol 1 (DY02), long procedure
  • Purification of total DNA from animal sperm using the DNeasy Blood and Tissue kit; protocol 2 (DY03), short procedure
  • Purification of DNA from epithelial cells mixed with sperm cells using the QIAamp DNA Micro Kit (QA40).
FAQ ID -909
What is the composition of buffer AE?

The composition of Buffer AE is:

  • 10 mM Tris-Cl
  • 0.5 mM EDTA; pH 9.0.
FAQ ID -730
How do I safely inactivate biohazardous flow-through material?

Always dispose of potentially biohazardous solutions according to your institution’s waste-disposal guidelines. Although the lysis and binding buffers in QIAamp, DNeasy, and RNeasy kits contain chaotropic agents that can inactivate some biohazardous material, local regulations dictate the proper way to dispose of biohazards. DO NOT add bleach or acidic solutions directly to the sample-preparation waste. Guanidine hydrochloride in the sample-preparation waste can form highly reactive compounds when combined with bleach.
Please access our Material Safety Data Sheets (MSDS) online for detailed information on the reagents for each respective kit.

FAQ ID -12