Multiplex PCR is a powerful technique that enables the detection of more than one target in a single reaction. This method maximizes the gain of biological information even with limited sample amounts, budgets and time. However, in typical multiplexing, the number or targets is limited by the number of available detection channels for the dyes used to label the target-specific probes. As a result, most qPCR and dPCR systems detect no more than six targets in parallel.

The QIAcuity Digital PCR System has overcome this limitation, enabling clear discrimination of up to 12 targets in a single reaction. This high-level multiplexing is made possible by specialized chemistry and advanced software with improved cross talk compensation, which corrects for signal overlap between targets.

In this webinar, Dr. Gerald Schock, Director Product Management dPCR at QIAGEN, will explain how the combination of sophisticated software and innovative chemistry enables the analysis of up to 12-plex reactions. He will also present examples of 6-plex, 8-plex and 12-plex experiments for copy number variation (CNV) analysis and pathogen detection. Finally, the Q&A session will help you integrate multiplexing in your lab to enhance productivity and extract more information from your experiments.