Unique rotary design for outstanding performance
The unique centrifugal design of the Rotor-Gene Q MDx makes it the most precise and versatile real-time PCR cycler currently available (see figure " Cross-section of the Rotor-Gene Q MDx"). Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. Detection is similarly uniform. When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis. The rotary design delivers up to 20 times better thermal precision in real-time cycling compared with traditional block-based instruments. Build up complete confidence in your real-time PCR test by using highly precise rotary technology.
Unrivaled optical range enables multiple applications
The optical channels of the Rotor-Gene Q MDx compliment QIAGEN's CE-IVD-marked assays. With up to 6 channels spanning UV to infrared wavelengths, the cycler is ready to support even the most demanding multiplex applications (see table). In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q MDx is now compatible with dyes you may want to use in the future.
Channels for optical detection Channel | Excitation (nm) | Detection (nm) | Examples of fluorophores detected |
Blue | 365 ± 20 | 460 ± 20 | Marina Blue, Edans, Bothell Blue, Alexa Fluor 350, AMCA-X, ATTO 390 |
Green | 470 ± 10 | 510 ± 5 | FAM, SYBR® Green I, Fluorescein, EvaGreen, Alexa Fluor 488 |
Yellow | 530 ± 5 | 557 ± 5 | JOE, VIC, HEX, TET, CAL Fluor Gold 540, Yakima Yellow |
Orange | 585 ± 5 | 610 ± 5 | ROX, CAL Fluor Red 610, Cy 3.5, Texas Red, Alexa Fluor 568 |
Red | 625 ± 5 | 660 ± 10 | Cyanine 670, Quasar 670, LightCycler Red640, Alexa Fluor 633 |
Crimson | 680 ± 5 | 712 high pass | Quasar 705, LightCycler Red705, Alexa Fluor 680 |
HRM | 460 ± 20 | 510 ± 5 | SYBR® Green I, SYTO9, LC Green, LC Green Plus+, EvaGreen |
Minimum maintenance; maximum convenience
The Rotor-Gene Q MDx is engineered to reduce the need for maintenance, and to maximize ease of use. This saves time and costs, and keeps the instrument operational for continuous testing. The outstanding robustness of Rotor-Gene technology is proven in several thousand installations worldwide.
Convenient features of the Rotor-Gene Q MDx include:
- Lifetime guarantee on highly stable LEDs, no expensive and time consuming lamp replacement, and no gradual performance loss
- Cost-effective and easy temperature-accuracy verification (see below)
- Optical calibration not required at installation, or when the instrument is moved
- "Plug and play" connectivity between the computer and the instrument
- No sample block to clean
- No condensation or bubbles in the reaction due to rotation
- Small, light, and robust instrument — simply place it where you want it