The purification procedure is designed to ensure safe and reproducible handling of precious or potentially infectious samples, and comprises 4 steps: lyse, bind, wash and elute. The user can choose different elution volumes between 30 μl to 400 μl of water or modified TE Buffer (Buffer ATE), depending on the protocol.
Since the type of samples that can be processed using the QIAsymphony DNA Investigator Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions in the presence of proteinase K and Buffer ATL in total volumes of either 200 μl, 500 μl or 1000 μl.
There are two different kinds of DNA purification protocols, which can be used in conjunction with the pretreatment protocols. Casework protocols purify genomic and mitochondrial DNA from 200 μl, 500 μl or 1000 μl of lysate obtained from the sample lysis procedures detailed in the corresponding pretreatment protocols. DNA can be eluted in 30–200 μl of either water or modified TE Buffer (Buffer ATE). Reference protocols purify DNA from database samples, such as buccal swabs or dried blood. DNA can be eluted in 100–400 μl of Buffer ATE. The purified DNA is ready to use in downstream applications.
QIAsymphony DNA Investigator protocols
|Protocol ||Lysis volume options ||Enhanced binding ||Eluate volume |
|Reference ||200, 500 μl ||No ||100–400 μl |
|Casework ||200, 500, 1000 μl ||No ||100–200 μl |
|Casework ADV ||200, 500, 1000 μl ||Yes ||100–200 μl |
|Casework HE ||200, 500, 1000 μl ||No ||30–80 μl |
|Casework HE ADV ||200, 500, 1000 μl ||Yes ||30–80 μl |