QIAseq FastSelect –5S/16S/23S Kits use a novel method to remove highly abundant bacterial 5S/16S/23S rRNA from your RNA-seq library. QIAseq FastSelect –5S/16S/23S was specifically designed for complex community microbial samples from soil, water, stool and sludge, which have numerous and diverse bacterial populations. The rRNA coverage provided by this kit is based on SILVA 16S sequences (nearly 600,000 entries), SILVA 23S sequences (nearly 170,000 entries) and 5S rRNA database sequences (over 7200 entries), and in-silico modeling shows coverage of >95% of all known 5S, 16S and 23S rRNA sequences.
QIAseq FastSelect –5S/16S/23S has been tested with QIAGEN, Illumina and NEB RNA-seq library kits and is compatible with all major methods of RNA-seq library preparation. The simple 14-minute protocol and bead cleanup integrates seamlessly with all common RNA-seq library kits, and can be used on fragmented or full-length (non-fragmented) RNA.
For removal of human, mouse, rat and other mammalian rRNA, please see our QIAseq FastSelect –rRNA HMR Kits.
Also, check out our QIAseq Stranded RNA Library Kits for robust RNA-seq library preparation.
Design your own custom QIAseq FastSelect pools to remove any RNAs you wish from your RNA-seq library – take a look at our QIAseq FastSelect Custom RNA Removal Kits.
Want to try this solution for the first time? Request a trial kit to evaluate.