The superior chemistry of the QIAseq Methyl Library Kit enables high library yields, using as little as 100 pg of input DNA for library construction, from a low number of amplification cycles (Figure High library yield and mapping rate
High mapping rates
One of the major challenges in the use of NGS for methylation analysis is low mapping rates, resulting in inefficient use of the sequencing run. The QIAseq Methyl Library Kit overcomes this challenge by delivering mapping rates greater than 90%, with an optimal DNA input range of 1–100 ng (Figure High library yield and mapping rate
Interrogates CpG methylation with low bias
Accurate methylation analysis is mostly affected by the input material and unbiased conversion of the bisulfite-treated and heavily-fragmented DNA into library molecules. The high degree of bisulfite conversion provided by EpiTect Fast and efficient repair of the bisulfite-treated DNA, coupled with efficient ligation of adapters, make accurate methylation analysis possible, even from very low DNA input (Figure Accurate methylation state calling
Low error rates
With an optimal input range of 1–100 ng DNA, the QIAseq Methyl Library Kit, which includes the high-fidelity VeraSeq ULtra DNA Polymerase, reduces error rates for more accurate assessment of methylation events.