PyroMark Q96 Tests

For analyzing gene-specific sequence variation or methylation on PyroMark Q96 ID or PyroMark Q96 MD

Detection and quantification of mutations in cancer-related genes
Quantification of individual or multiple CpG sites in different genes
Estimation of global CpG methylation in defined regions of interest
Sequence context provides built-in quality control of the assays

PyroMark Q96 Tests are a range of predesigned assays suitable for highly reliable, quantitative analysis of mutations or SNPs, as well as CpG methylation. Tests designated specifically with 'Q96' are optimized for use with the PyroMark Q96 MD and/or PyroMark Q96 ID Instruments. Other tests, such as PyroMark HFE and PyroMark MTHFR, can additionally be performed on the PyroMark Q24 (with the exception of PyroMark APOE).

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Cat No./ID: 972012

PyroMark Q96 CpG p16 (96)

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For quantification of methylation level in the p16 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)

Cat No./ID: 972022

PyroMark Q96 CpG MLH1 (96)

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For quantification of methylation level in promoter region of the MLH1 gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)

Cat No./ID: 972032

PyroMark Q96 CpG MGMT (96)

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For quantification of methylation level in the MGMT gene (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)

Cat No./ID: 972052

PyroMark Q96 CpG Prader-Willi (96)

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For quantification of methylation level in Prader-Willi and Angelman Syndromes (2 x 96 reactions on PyroMark Q96 MD and 1 x 96 reactions on PyroMark Q96 ID)

Cat No./ID: 972422

PyroMark APOE (96)

For detection of polymorphism in the APOE gene (2 x 96 reactions on PyroMark Q96 MD or 1 x 96 reactions on PyroMark Q96 ID)

Cat No./ID: 972432

PyroMark MTHFR (96)

For detection of variants in the MTHFR gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)

Cat No./ID: 972442

PyroMark HFE (96)

For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)

Cat No./ID: 973043

PyroMark Q96 CpG LINE-1 (2 x 96)

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For quantification of global methylation level in transposable elements; only for use with PyroMark Q96 MD

PyroMark APOE, PyroMark HFE, PyroMark MTHFR, PyroMark Q96 CpG LINE-1, PyroMark Q96 CpG MGMT, PyroMark Q96 CpG MLH1, PyroMark Q96 CpG p16, and PyroMark Q96 CpG Prader-Willi tests are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

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PyroMark p16 analysis.

[A] A normal blood DNA sample and [B] a DNA sample isolated from a colon tumor were analyzed. Blue columns indicate the measured CpG sites, and the individually measured degree of methylation at each site. Yellow columns indicate QC for completion of the bisulfite treatment.

Performance

The PyroMark Q96 Tests are highly suitable for accurately measuring the CpG methylation status, as exemplified with the PyroMark Q96 CpG p16 Test (see figure PyroMark p16 analysis).

Principle

PyroMark Q96 Tests used in combination with PyroMark Q96 MD and/or PyroMark Q96 ID Systems enable accurate, quantitative mutation (or SNP) and methylation analysis by Pyrosequencing. The assays consist of PCR primers and a sequencing primer.

Procedure

Prior to Pyrosequencing, a biotinylated PCR product is generated. Biotinylated PCR products are bound to streptavidin-coated Sepharose beads. The beads are then captured with the vacuum tool on the PyroMark Vacuum Workstation, and throroughly washed, generating single-stranded DNA suitable for sequencing. PyroMark Q96 Tests are predesigned assays for methylation analysis by Pyrosequencing. Each test includes the primers necessary for generating the biotinylated PCR product, as well as the primer for the Pyrosequencing reaction.

Applications

PyroMark Q96 Tests enable highly accurate, quantitative methylation and mutation (or SNP) analysis applicable in several fields of research, including cancer and epigenetics.

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FAQs (17)

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	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	View
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	View
	What is the amplicon length of the PyroMark CpG LINE assay? -1	View
	What region of LINE gets targeted by the assay for PyroMark Q96 MD or PyroMark Q24? -1	View
	How many CpG sites are analyzed by the PyroMark CpG LINE assay? -1	View
	Does the PyroMark CpG LINE assay target mouse transposons as well? -1	View
	Does the PyroMark LINE assay target all LINE sequences? -1	View
	Can the PyroMark Q96 CpG LINE assay be used with an ID system? -1	View
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	View
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	View
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	View
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	View
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	View
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	View
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	View
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	View
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	View

Scientific Posters (1)

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	(EN) - Pyrosequencing and its applications	Show details

Kit Handbooks (4)

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	(EN) - PyroMark APOE Handbook	Show details
	(EN) - PyroMark HFE Handbook	Show details
	(EN) - PyroMark MTHFR Handbook	Show details
	(EN) - PyroMark Q96 CpG LINE-1 Handbook	Show details

Brochures & Guides (1)

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	Pyrosequencing – the synergy of sequencing and quantification	Show details

Safety Data Sheets (8)

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	MSDS PyroMark APOE (96)
	MSDS PyroMark HFE (96)
	MSDS PyroMark MTHFR (96)
	MSDS PyroMark Q96 CpG LINE-1 (2 x 96)
	MSDS PyroMark Q96 CpG MGMT (96)
	MSDS PyroMark Q96 CpG MLH1 (96)
	MSDS PyroMark Q96 CpG p16 (96)
	MSDS PyroMark Q96 CpG Prader-Willi (96)

References