artus Infl./H1 LC/RG RT-PCR Kit RUO
For detection of influenza A/B RNA and specific detection of influenza A (H1N1) RNA (pandemic 2009)
The artus Infl./H1 LC/RG RT-PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit includes a reaction mix containing all reagents and enzymes for amplification and detection of all known influenza A and B viruses. In addition, the kit contains an extra reaction mix for the specific identification of influenza A (H1N1) virus.
Principle
The artus Infl./H1 LC/RG RT-PCR Kit is based on the amplification and simultaneous detection of specific regions of the influenza virus genome using real-time PCR. Each artus Infl./H1 LC/RG RT-PCR Kit provides positive controls for influenza and for influenza H1. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR.
Procedure
The artus Infl./H1 LC/RG RT-PCR Kit provides all necessary reagents optimized for reliable influenza RNA detection. Simply add template RNA to the ready-to-use PCR master mixes and Mg solution, and start the reactions on the appropriate real-time cycler using the optimized cycling program described in the kit handbook. For RNA purification, we recommend using the QIAamp Viral RNA Mini Kit, QIAamp MinElute Virus Spin Kit, or EZ1 DSP Virus Kit.
Applications
The artus Infl./H1 LC/RG RT-PCR Kit allows the rapid and sensitive detection of influenza virus RNA from sputum, throat, and nasal swabs. The kit is available for use on Rotor-Gene Q instruments or the LightCycler 1.1/1.2/1.5/2.0 Instruments.
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