Can Ni-NTA resins be used to purify protein with an internal His-tag?

Yes, Ni-NTA Agarose and Superflow will bind a 6xHis-tag whether it is located internally or at the C- or N-teminal end of the protein. Note that the His-tag must be exposed for binding at the surface of the protein to allow for efficient purification under native conditions.
icon_0011_idea-s

Can’t find what you are looking for?

Browse the FAQ base with our FAQ search.