Why is there no detection of free reporter fluorescence in the LiquiChip System?
FAQ ID -732

The detection of the green reporter signal is triggered by the detection of the red classification fluorescence. Therefore, a specific sample value is only recorded if a red bead classification signal and a green reporter signal are measured simultaneously.

In addition, the assay mixture is covered by the LiquiChip System Fluid for the measurement in the capillary, so that only a small amount of assay mixture is around the beads during measurements. Further information can be found online on the product page for the LiquiChip System.