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BioSprint 15 DNA Blood Kit

For rapid and economical DNA purification from a wide range of sample types
  • Complete removal of contamination and inhibitors
  • Purification of high-quality DNA that is ready for use
  • Automated procedure saves time and effort
  • No organic extraction or alcohol precipitation

The BioSprint 15 DNA Blood Kit provides all reagents and plasticware necessary for automated purification of genomic DNA from cultured cells, tissues, blood, dried blood spots, and buccal swabs using magnetic particle technology on the BioSprint 15.

Cat No./ID: 940014
BioSprint 15 DNA Blood Kit (45)
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For 45 preps: 5-Rod Covers, 5-Tube Strips, MagAttract Suspension G, Buffers and Reagents
Cat No./ID: 940017
BioSprint 15 DNA Blood Kit (360)
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For 360 preps: 5-Rod Covers, 5-Tube Strips, MagAttract Suspension G, Buffers and Reagents
The BioSprint 15 DNA Blood Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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BioSprint DNA procedure.
1
Reproducible purification of DNA from sheep ear tissue.
Sheep ear tissue samples were treated with 180 µl Buffer ATL and 20 µl QIAGEN Proteinase K at 56°C overnight. Genomic DNA was purified from the lysed tissue samples using the BioSprint 15 DNA Blood Kit with the BioSprint 15 DNA Tissue protocol. DNA was eluted in 200 µl elution buffer. Eluates (5 µl) were run on a 0.8% agarose gel in 1x TBE. M: markers (Lambda HindIII).
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High PCR performance of DNA purified using the BioSprint 15 DNA Blood Kit.
Human blood was collected and treated with one of 3 anticoagulants: citrate (C), EDTA (E), or heparin (H). DNA was purified from 200 µl blood immediately after blood collection (Fresh) and after one cycle of freezing and thawing (Frozen). DNA was eluted in 200 µl elution buffer. Eluates (1 µl and 10 µl) were subjected to PCR (50 µl reaction volume) using primers to amplify a 1 kb fragment of the Hugl gene. PCR products were run on a 1.5% agarose gel in 1x TBE. M: markers.
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Efficient processing of magnetic particles.

The BioSprint instrument transfers magnetic particles from one well or tube to another.

Performance

DNA can be successfully purified from a variety of sample types (see table).

Typical yields from a range of sample types
Sample typeAmount of sample DNA (µg)
Muscle, bovine 25 mg 16.2 ± 2.5
Heart, bovine 25 mg 5.9 ± 2.6
Spleen, bovine 25 mg 69.1 ± 23.6
Lung, bovine 25 mg 13.8 ± 7.2
Liver, bovine 25 mg 77.8 ± 29.4
Kidney, bovine 25 mg 26.2 ± 18.8
Ear, sheep 30 mg 20.31 ± 1.8
Tail, mouse 1.2 cm (~25 mg) 32.7 ± 4.6
HL-60 cultured cells 2 x 106 cells 10.1 ± 4.7
Blood, human
(5–7 x 106 cells per ml)		 200 µl 4.5–9.0
Blood, bovine 200 µl 6.2–8.0
Blood, sheep 200 µl 5.6–11.2
Blood, pig 200 µl 4.5–9.0
Blood, dog 200 µl 6.7–12.3
Blood, cat 100 µl 4.4–8.3
Blood, mouse 100 µl 2.0–8.0
Blood, rat 100 µl 1.0–4.0
Blood, bird 10 µl* 15.0 ±6.3
Blood, fish 5 µl* 7.1–10.0
Dried blood spots, 903 Specimen Collection Paper 1 punch (6 mm [1/4 inch] diameter) 0.30
Dried blood spots, FTA Card 1 punch (6 mm [1/4 inch] diameter) 0.12
Buccal swabs 1 swab 0.8–2.0

Genomic DNA was purified from the indicated samples. DNA was eluted in 200 µl Buffer AE.
* Sample diluted to 200 µl.

Purified DNA is up to 50 kb in size (see figure "Reproducible purification of DNA from sheep ear tissue"). The DNA is ready to use in all downstream applications, including Southern blotting and enzyme applications such as PCR (see figure "High PCR performance of DNA purified using the BioSprint 15 DNA Blood Kit").

Principle
The BioSprint 15 DNA Blood Kit is used with the BioSprint 15. The kit uses magnetic-particle technology for rapid DNA purification from cells, tissue, and blood or blood-related products. The BioSprint 15 controls an array of magnetic rods that can attract or release magnetic particles and transfer them from tube to tube. The sequential transfer of magnetic particles allows a rapid purification procedure to be performed, from the initial binding of DNA to the particles, through to washing of the particles and elution of pure DNA (see figure "Efficient processing of magnetic particles"). Since the instrument transfers magnetic particles instead of liquids, it uses minimal amounts of reagents, enabling cost-efficient sample preparation.
Procedure

Genomic DNA can be purified from cells, tissue, and blood. Lysed samples are loaded into the instrument, which then performs all steps until pure DNA is obtained (see figure "BioSprint DNA procedure"). The kit provides all the necessary reagents and plasticware for sample preparation, and is available in different sizes, allowing cost-efficient use of consumables.

For purification of genomic DNA from tissue, dried blood spots, or buccal swabs, Buffer ATL and QIAGEN Proteinase K are required (not supplied with the BioSprint 15 DNA Blood Kit).

Applications

The BioSprint 15 DNA Blood Kit purifies DNA for use in a range of downstream applications, including:

Southern blotting
PCR and real-time PCR
Genotyping 
Features
Specifications
Applications PCR, real-time PCR, genotyping, blotting
Elution volume 200 µl
For automated processing BioSprint 15 workstation
Format 5-tube strip, S-Block
Main sample type Blood, tissue, cells
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount 100 µl/25 mg/2 x 10e6
Technology Magnetic particles
Time per run or per prep 45 minutes
Yield Varies

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Brochures & Guides (1)
(EN) - Models of efficiency — the affordable BioSprint 15 and BioSprint 96
Rapid and economical preparation of 1–15 or 1–96 samples using magnetic-particle technology
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Kit Handbooks (1)
(EN) - BioSprint 15 DNA Handbook (June 2012)
For purification of DNA from human whole blood, animal whole blood, buffy coat, cultured cells, tissues, rodent tails, buccal swabs, and dried blood spots using the BioSprint 15 workstation
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