FAQ-894
Do you have a protocol for the isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits?
Yes, please follow the Supplementary Protocol 'Isolation of single-stranded DNA from M13 phage using QIAGEN Plasmid Kits' (QP14).
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QIAGEN Plasmid Kits
QIAGEN Plasmid Kits provide gravity-flow, anion-exchange tips for purification of transfection-grade plasmid DNA. Lysate clearing and isopropanol precipitation are achieved by centrifugation.
The QIAGEN Plasmid Mega Kit (cat. no. 12181) and the QIAGEN Plasmid Giga Kit (cat. no. 12191) can be used with the QIAfilter Mega-Giga Cartridges (cat. no. 19781) as an optional protocol step for rapid clearing of bacterial lysates by filtration instead of centrifugation. See more information regarding principle below.