PyroMark Q96 ID Accessories and Reagents

For optimal sample preparation and Pyrosequencing analysis using PyroMark Q96 ID

Reliable quantification of genetic or epigenetic DNA modifications
Solutions for quick and easy sample handling for successful results
Dedicated products for each platform and each step in the workflow
High-quality optimized reagents enable reliable Pyrosequencing results

The PyroMark Q96 ID uses proven Pyrosequencing technology for real-time, sequence-based detection and quantification for genetic analysis and epigenetic methylation studies. PyroMark Q96 ID Accessories and Reagents are high-quality reagents and products that simplify and streamline the Pyrosequencing workflow and enable successful results. PyroMark Q96 ID Accessories and Reagents are designed specifically for use with PyroMark Q96 ID instruments.

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Product	Product no.	Cat. no.	List price:
PyroMark Q96 Vacuum Workstation (110 V) Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template show details	9001528		Inquire	add to cart add to cart
PyroMark Q96 Vacuum Workstation (220 V) Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template show details	9001529		Inquire	add to cart add to cart
PyroMark Q96 Vacuum Workstation (100 V) Vacuum workstation for preparing 96 samples in parallel from PCR product to single-stranded template show details	9001740		Inquire	add to cart add to cart
PyroMark Q96 Sample Prep Thermoplate Low Holder for placement of PyroMark Q96 Plate Low on heating block during annealing step prior to PyroMark Q96 ID analysis show details	9019070		Inquire	add to cart add to cart
PyroMark Gold Q96 Reagents (5 x 96) For performing Pyrosequencing reactions on the PyroMark Q96 ID (5 x 96) and PyroMark Q96 MD (15 x 96) show details	972804		Inquire	add to cart add to cart
PyroMark Gold Q96 SQA Reagents (1 x 96) For performing Pyrosequencing reactions on the PyroMark Q96 ID show details	972812		Inquire	add to cart add to cart
PyroMark PCR Kit (200) For 200 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water show details	978703		Inquire	add to cart add to cart
PyroMark PCR Kit (800) For 800 reactions: 2x PyroMark PCR Master Mix (includes HotStarTaq DNA Polymerase and optimized PyroMark Reaction Buffer containing 3 mM MgCl₂ and dNTPs), 10x CoralLoad Concentrate, 5x Q-Solution, 25 mM MgCl₂, and RNase-Free Water show details	978705		Inquire	add to cart add to cart
PyroMark CpG Assay (200) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; tube format) show details	978746		Inquire	Create and order Create and order
PyroMark CpG Assay 96 plate (50) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (50 reactions; plate format); minimum order of 24 assays per plate show details	978754		Inquire	Create and order Create and order
PyroMark CpG Assay 96 plate (200) PCR and sequencing primers for Pyrosequencing analysis of gene-specific CpG methylation after DNA bisulfite conversion (200 reactions; plate format); minimum order of 24 assays per plate show details	978756		Inquire	Create and order Create and order
PyroMark Custom Assay (200) User-designed PCR and sequencing primers for Pyrosequencing analysis (200 reactions; tube format) show details	978776		Inquire	Create and order Create and order
PyroMark OneStep RT-PCR Kit (50) For 50 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water show details	978801		Inquire	add to cart add to cart
PyroMark OneStep RT-PCR Kit (200) For 200 reactions: QIAGEN OneStep RT-PCR Enzyme Mix, optimized QIAGEN OneStep RT-PCR Buffer, 10x CoralLoad Concentrate, 5x Q-Solution, dNTP Mix, and RNase-Free Water show details	978803		Inquire	add to cart add to cart
PyroMark Q96 Plate (100) For sample analysis of DNA template prepared with magnetic beads; for use with PyroMark Q96 ID show details	979001		Inquire	add to cart add to cart
PyroMark Q96 Plate Low (100) For sample analysis of DNA template on PyroMark Q96 ID show details	979002		Inquire	add to cart add to cart
PyroMark Q96 Sample Prep Tool Cover (10) Consumables for use with magnetic tool for isolation of single stranded DNA; for use with PyroMark Q96 ID show details	979003		Inquire	add to cart add to cart
PyroMark Q96 Cartridge (3) For delivery of nucleotides and reagents for use with PyroMark Q96 ID show details	979004		Inquire	add to cart add to cart
PyroMark Binding Buffer (200 ml) For binding of biotinylated PCR product to Sepharose beads show details	979006		Inquire	add to cart add to cart
PyroMark Denaturation Sol. (500 ml) For denaturation of double-stranded PCR product into single-stranded DNA template show details	979007		Inquire	add to cart add to cart
PyroMark Wash Buffer (conc., 200 ml) For use with the PyroMark Q24 Vacuum Workstation and PyroMark Q96 Vacuum Workstation to wash and neutralize the immobilized DNA show details	979008		Inquire	add to cart add to cart
PyroMark Annealing Buffer For annealing of sequencing primer to DNA template show details	979009		Inquire	add to cart add to cart
PyroMark Q96 Vacuum Prep Trough (5) Reusable plastic troughs for use with all PyroMark Q96 Vacuum Workstations show details	979011		Inquire	add to cart add to cart
PyroMark Control Oligo For installation check of system show details	979203		Inquire	add to cart add to cart

PyroMark Q96 ID Accessories and Reagents are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

Applications

PyroMark Q96 ID Accessories and Reagents enable Pyrosequencing analysis with the PyroMark Q96 ID instrument for applications such as:

HRM
Methylation analysis
Mutation analysis
SNP analysis

FAQs (25)

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	Can I order the nucleotides from PyroMark Gold Reagents separately? FAQ ID -2827	View
	How many times can vacuum troughs be re-used with the PyroMark Vacuum Preparation Stations? FAQ ID -2848	View
	How many nucleotides of a homopolymer can be resolved in pyrosequencing? FAQ ID -2871	View
	Can unused wells in a pyrosequencing plate be used in the next run? FAQ ID -2872	View
	What is the reason for signals ceasing in the middle of a pyrosequencing run? FAQ ID -2875	View
	How do I reduce background peaks in the pyrosequencing pyrogram? FAQ ID -2877	View
	How do I prevent a drifting baseline in my pyrosequencing pyrogram? FAQ ID -2878	View
	What is the reason for a high substrate peak in the pyrosequencing pyrogram? FAQ ID -2879	View
	What is the reason for split peaks appearing in between dispensations on my pyrosequencing pyrogram? FAQ ID -2881	View
	Which purity grade is recommended for pyrosequencing primers? FAQ ID -2832	View
	What concentration should be used for the sequencing primer in pyrosequencing? FAQ ID -2826	View
	Where can I order the Streptavidin Sepharose beads for pyrosequencing? FAQ ID -2850	View
	How accurate and reliable is PyroMark Q24 in mutation analysis? FAQ ID -2094	View
	What kind of shaker should be used for the pyrosequencing binding step? FAQ ID -2837	View
	Which heating block is recommended for the pyrosequencing annealing step? FAQ ID -2838	View
	Can PyroMark Gold reagents be vortexed? FAQ ID -2844	View
	What is the concentration of PyroMark Control Oligo? FAQ ID -2846	View
	What is the use of the PyroMark Q24 Validation Oligo? FAQ ID -2855	View
	How many times can the cartridges for Pyromark Q24 or PyroMark Q96 ID instruments are reused? FAQ ID -2863	View
	Which kits can be used in combination with the PyroMark CpG Assays and PyroMark Custom Assays? FAQ ID -2822	View
	What should be the single peak height for the PyroMark Control Oligo on the different PyroMark instruments? FAQ ID -2852	View
	Which PyroMark Gold Q96 Reagent should be used for which instrument and application? FAQ ID -2836	View
	What is the meaning of the abbreviations NDT and CDT and what are the differences? FAQ ID -2841	View
	How many times can the CDTs, NDTs, and RDTs be used? FAQ ID -2865	View
	Does the Pyromark Assay Design or application software give any cycling conditions for individual assay primers or a PCR setup pipetting scheme? FAQ ID -2862	View

Brochures & Guides (2)

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	Product Profile - PyroMark Q24 Advanced	Show details
	Pyrosequencing – the synergy of sequencing and quantification	Show details

Kit Handbooks (5)

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	PyroMark Control Oligo Handbook For use with PyroMark Q48 Autoprep, PyroMark Q24 Advanced, PyroMark Q24, PyroMark Q96 ID and PyroMark Q96 MD systems	Show details
	(EN) - PyroMark Gold Q96 Reagents Handbook For performing Pyrosequencing reactions on the PyroMark Q96 ID, PyroMark Q96 MD, and PyroMark Q96 MD Automated	Show details
	(EN) - PyroMark OneStep RT-PCR Handbook	Show details
	(EN) - PyroMark PCR Handbook	Show details
	(EN) - PyroMark Q96 Cartridge Product Sheet	Show details

User-Developed Protocols (1)

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	Pyrosequencing® method for the forensic identification of tissue source using QIAGEN’s PyroMark® Q24 Advanced system - (EN)	Show details

White Papers (1)

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	(EN) - Techniques to overcome bottlenecks in epigenetics research	Show details

Application Notes (2)

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	Pyrosequencing – tissue source forensic identification using PyroMark Q24 Advanced A Pyrosequencing method for the forensic identification of tissue source using QIAGEN’s PyroMark Q24 Advanced system.	Show details
	Tissue source attribution using the PyroMark Q48 Autoprep System: Sperm identification in forensic casework	Show details

Quick-Start Protocols (2)

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	(EN) - PyroMark PCR Kit Quick-Start Protocol	Show details
	PyroMark Q96 Vacuum Workstation Quick-Start Guide - (EN)	Show details

Safety Data Sheets (12)

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	MSDS PyroMark Annealing Buffer
	MSDS PyroMark Binding Buffer (200 ml)
	MSDS PyroMark Control Oligo
	MSDS PyroMark Denaturation Sol. (500 ml)
	MSDS PyroMark Gold Q96 Reagents (5 x 96)
	MSDS PyroMark Gold Q96 SQA Reagents (1 x 96)
	MSDS PyroMark OneStep RT-PCR Kit (200)
	MSDS PyroMark OneStep RT-PCR Kit (50)
	MSDS PyroMark PCR Kit (200)
	MSDS PyroMark PCR Kit (800)
	MSDS PyroMark Wash Buffer (conc., 200 ml)
	CofA

References