Concentration of RNA.

Reliable cleanup of RNA.

Efficient removal of real-time RT-PCR inhibitors.

Efficient removal of real-time RT-PCR inhibitors.

High-quality RNA.

The indicated amounts of total RNA from HeLa cells were concentrated using the RNeasy MinElute Cleanup Kit, and 5 µl of the 12 µl eluate was used for each reaction. Real-time RT-PCR was carried out on an ABI Sequence Detection System using the QuantiTect Probe RT-PCR Kit, with primers and probes specific for β-actin.

The indicated amounts of total RNA from HeLa cells were concentrated using the RNeasy MinElute Cleanup Kit. Real-time RT-PCR was carried out on the LightCycler System using the QuantiTect Probe RT-PCR Kit, with primers and probes specific for the p16 gene.

Efficient removal of real-time RT-PCR inhibitors.

Without cleanup, inhibitors can lead to underestimation of RNA amounts in real-time quantification. Total RNA was isolated from HeLa cells using an acid-phenol method, with or without subsequent cleanup using the RNeasy MinElute Cleanup Kit. Real-time RT-PCR was carried out as described in figure "Concentration of RNA", with the indicated volumes of the RNA eluate. Apparent RNA amounts were calculated from the resulting C_T values.

Total RNA was isolated from HeLa cells using an acid-phenol method plus cleanup and concentration using the RNeasy MinElute Cleanup Kit. The high quality of the RNA is shown by scanning with the Agilent 2100 Bioanalyzer.