Automated purification with the EZ1 RNA Tissue Mini Kit requires use of the EZ1 Advanced XL and the EZ1 Advanced XL RNA Card, or the EZ1 Advanced and the EZ1 Advanced RNA Card, or the BioRobot EZ1 and the EZ1 RNA Card. EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure.
Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1-14 samples (EZ1 Advanced XL) or 1-6 samples (EZ1 Advanced and BioRobot EZ1) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination or degradation due to RNases. The automated procedure provides high repeatability and linear yields over a range of starting amounts. The flexibility to purify high-quality total RNA from a wide range of tissue types ensures that samples can be purified for a range of gene expression analysis applications. RNA yield using the EZ1 RNA Tissue Mini Kit is dependent upon many variables, including sample weight, tissue type and developmental state.