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PCR/qPCR/dPCR

Specific, sensitive and robust PCR with confidence

When performing gene amplification, your experiments’ success can depend on several critical factors, including the throughput, assay sensitivity, robustness of the reaction even with difficult templates, and reliable data normalization. You may also need to overcome the specific challenges associated with the PCR method you are using.

Whichever method you choose, we offer a wealth of optimized solutions for end-point PCR, qPCR and dPCR. Our kits, assays, instruments and accessories help you amplify and detect targets with confidence and streamline your workflow for virtually any application.

  • Measure gene expression (mRNA, miRNA)
  • Quantify DNA or RNA targets
  • Detect & quantify pathogen and/or viral RNA/DNA
  • Quantify residual DNA
  • Convert RNA to cDNA for quantification
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  • Mutation detection (including rare/ultra-rare variants)
  • Analyze copy number variation (CNV)
  • Detect viral load
  • Gene expression quantification
  • Quantify low-abundance targets
  • Measure residual DNA
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  • Amplify DNA for cloning or sequencing
  • Qualitative pathogen detection (presence/absence)
  • CRISPR screening
  • Genotyping
  • Convert RNA to cDNA for storage
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  • Cq/Ct values
  • Relative quantification (ΔΔCq) or absolute quantification from standards
  • cDNA for downstream use
  • Absolute target quantification (copies/µL reaction)
  • Fractional abundance (%)
  • Mutation/target frequency
  • Copy number per genome
  • Amplified DNA or cDNA product
  • Band visualization on gel
  • Presence/absence result
  • Isolated DNA or cDNA
  • Isolated RNA/mRNA/miRNA
  • Some kits support crude samples such as cells, blood, dried blood spots, saliva, buccal swabs, or gargle
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  • Isolated DNA or cDNA
  • Isolated RNA/mRNA/miRNA
  • Purified samples recommended
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  • Isolated DNA or cDNA
  • Isolated RNA
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  • Quantitative real-time PCR
  • Reverse transcription
  • Amplification and fluorescence-based detection in real-time for quantification
  • Quantitative digital PCR
  • Partitions the sample into thousands of reactions and uses end-point PCR + Poisson statistics for absolute quantification without a standard curve
  • Standard end-point PCR
  • DNA amplification and visualization (e.g., gel-based) and downstream applications
  • When reliable quantification is required over a wide linear range
  • When standard curve-based or relative quantification is sufficient
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  • When precise quantification is required
  • When detecting rare alleles or small fold changes
  • When standard curves are undesirable
  • When working near the detection limit (low-abundance targets)
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  • When qualitative detection without quantification is sufficient
  • When preparing amplicons for cloning, genotyping, sequencing, or other downstream applications
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  • Real-time PCR thermal cycler
  • Dye-based (e.g., SYBR Green) or probe-based detection
  • QIAcuity dPCR platform
  • Probe‑based or intercalating dye detection
  • Thermal cycler
  • Visualization (e.g., via agarose gel electrophoresis)
Browse PCR/qPCR/dPCR

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104 Products found
ID SOLUTIONS IDNAPTEX dPCR Assays
ID SOLUTIONS IDNAPTEX dPCR Assays
For detecting genomic alterations, such as point mutations, deletions and fusions in DNA extracted from liquid and tissue biopsies using multiplex digital PCR for cancer research applications
QIAcuity Digital PCR System
QIAcuity Digital PCR System
For nanoplate-based digital PCR applications
QuantiTect PCR Kits
QuantiTect PCR Kits
For qPCR and two-step qRT-PCR using SYBR Green I and for qPCR and two-step qRT-PCR and also multiplex qPCR and two-step multiplex qRT-PCR using sequence-specific probes
QuantiTect RT-PCR Kits
QuantiTect RT-PCR Kits
For one-step qRT-PCR using SYBR® Green I and one-step qRT-PCR and multiplex qRT-PCR using sequence-specific probes for gene expression analysis
QuantiNova PCR Kits
QuantiNova PCR Kits
For highly sensitive, specific and ultrafast probe-based real-time PCR and multiplex PCR, and unparalleled results using SYBR Green-based qPCR
miRCURY LNA Digital PCR Assays
miRCURY LNA Digital PCR Assays
For extremely sensitive and specific miRNA quantification using QIAcuity EvaGreen-based dPCR
QuantiNova RT-PCR Kits
QuantiNova RT-PCR Kits
For highly sensitive and specific one-step qRT-PCR and multiplex qRT-PCR using sequence-specific probes or one-step qPCR using SYBR® Green I for gene expression analysis
Custom Microbial DNA qPCR Arrays
Custom Microbial DNA qPCR Arrays
For real-time PCR-based, customized microbial identification or profiling
dPCR Microbial DNA Detection Assays
dPCR Microbial DNA Detection Assays
For digital PCR detection of microbial targets, including bacterial, fungal, parasitic, viral, antibiotic resistance or virulence factor genes
REPLI-G Kits
REPLI-G Kits
For highly uniform whole genome amplification from 10ng of genomic DNA
QIAcuity High Multiplex Probe PCR Kit
QIAcuity High Multiplex Probe PCR Kit
For challenging samples and dPCR multiplexing beyond five targets
FastLane Cell RT-PCR Kits
FastLane Cell RT-PCR Kits
For SYBR® Green real-time one-step RT-PCR analysis and probe-based real-time one-step RT-PCR and multiplex RT-PCR analysis from cultured cells without RNA purification
QIAcuity OneStep Advanced EG Kit
QIAcuity OneStep Advanced EG Kit
For dye-based one-step RT-PCR on the QIAcuity digital PCR instruments
miRCURY LNA dPCR Custom Assays for miRNA
miRCURY LNA dPCR Custom Assays for miRNA
For extremely sensitive and specific miRNA quantification using QIAcuity EvaGreen-based dPCR
Q-Solution Kit
Q-Solution Kit
This kit includes Q-Solution and MgCl2 for obtaining better results from PCR reactions with more challenging setups.
QIAcuity Mycoplasma Quant Kit and Standards
QIAcuity Mycoplasma Quant Kit and Standards
For digital PCR quantification and detection of mycoplasma contamination using the QIAcuity Digital PCR System
dPCR Copy Number Variation (CNV) Probe Assays
dPCR Copy Number Variation (CNV) Probe Assays
For locus-specific analysis of copy number variations and alterations by singleplex or multiplex dPCR
mericon Sample Preparation and Bacteria Kits
mericon Sample Preparation and Bacteria Kits
For extraction of high-quality DNA from microbial enrichment cultures and raw or processed foods
QIAcuity RCL Quant Kit
QIAcuity RCL Quant Kit
For the detection of Replication Competent Lentivirus (RCL) with high accuracy using the QIAcuity digital PCR instruments
UCP PCR Kits
UCP PCR Kits
For SYBR Green-based and probe-based, real-time quantitative PCR for microbiome or quality control applications using reagents from Ultra Clean Production
QIAprep&amp Plasmodium Kit
QIAprep&amp Plasmodium Kit
For direct qPCR detection of Plasmodium spp. from dried blood spots or whole blood.
QIAprep&amp Plasmodium Assays
QIAprep&amp Plasmodium Assays
For distinguishing between Plasmodium falciparum and non-falciparum species, as well as differentiating four additional species
VeraSeq™ ULtra DNA Polymerase
VeraSeqâ„¢ ULtra DNA Polymerase
Ultrathermostable uracil-literate polymerase for high-fidelity PCR
QIAcuity mericon Food Testing Kits
QIAcuity mericon Food Testing Kits
For digital PCR authentication of food and animal feed using QIAcuity
dPCR PanCancer Kits
dPCR PanCancer Kits
For detection of multiple hallmark mutations in cancer-relevant genes using the QIAcuity digital PCR instruments
VeraSeq™ PCR Mix
VeraSeqâ„¢ PCR Mix
Ultrathermostable mastermix for high-fidelity PCR
mericon Ingredient Authentication Assay
mericon Ingredient Authentication Assay
For real-time PCR authentication of food and animal feed ingredients
QIAGEN OneStep RT-PCR Kit
QIAGEN OneStep RT-PCR Kit
For highly sensitive and specific one-step RT-PCR
QIAGEN Multiplex PCR Kit
QIAGEN Multiplex PCR Kit
For highly specific and sensitive multiplex PCR without optimization requirements
HotStarTaq DNA Polymerase
HotStarTaq DNA Polymerase
For highly specific amplification with minimal optimization
HotStarTaq Master Mix Kit
HotStarTaq Master Mix Kit
For highly specific amplification for any PCR application
Rotor-Gene Q (CA)
Rotor-Gene Q (CA)
For outstanding performance in real-time PCR
RT2 Profiler PCR Arrays
RT2 Profiler PCR Arrays
For pathway-focused gene expression analysis using laboratory-verified assays
QuantiFast Pathogen +IC Kits
QuantiFast Pathogen +IC Kits
For sensitive and reliable detection of viral RNA/DNA and bacterial DNA, including internal control
RT2 SYBR® Green qPCR Mastermixes
RT2 SYBR® Green qPCR Mastermixes
For use with RT2 Profiler, RT2 lncRNA, EpiTect ChIP, and EpiTect Methyl II PCR Arrays, and qPCR Assays
QIAGEN Multiplex PCR Plus Kit
QIAGEN Multiplex PCR Plus Kit
For straightforward and successful multiplex PCR in advanced applications
AllTaq Master Mix and PCR Core Kits
AllTaq Master Mix and PCR Core Kits
For ultrafast and versatile hot-start PCR in all applications
Taq DNA Polymerase
Taq DNA Polymerase
Taq DNA Polymerase delivers fast PCR set up with minimal optimization for efficient amplification of routine, low-copy, longer or difficult targets
QuantiNova Pathogen +IC Kit
QuantiNova Pathogen +IC Kit
For ultrafast, simultaneous detection of viral RNA/DNA and bacterial DNA, including internal control
Type-it Microsatellite PCR Kit
Type-it Microsatellite PCR Kit
For fast and reliable multiplex PCR analysis of microsatellite loci
RT2 First Strand Kit
RT2 First Strand Kit
For cDNA synthesis and genomic DNA elimination in RNA samples for use with RT2 Profiler PCR Arrays and RT2 lncRNA PCR Arrays
QuantiTect Virus Kits
QuantiTect Virus Kits
For highly sensitive detection of viral RNA and/or DNA
Omniscript RT Kit
Omniscript RT Kit
For reverse transcription of 50 ng to 2 μg RNA per reaction for end-point PCR
RT2 qPCR Primer Assays
RT2 qPCR Primer Assays
For accurate and reliable gene expression analysis using laboratory-verified assays
Sensiscript RT Kit
Sensiscript RT Kit
For reverse transcription of less than 50 ng RNA per reaction for end-point PCR
Custom RT2 Profiler PCR Arrays
Custom RT2 Profiler PCR Arrays
For reliable and sensitive gene expression profiling using laboratory-verified assays
HotStarTaq Plus DNA Polymerase
HotStarTaq Plus DNA Polymerase
For fast and highly specific amplification in all applications
miRCURY LNA miRNA Custom PCR Panels
miRCURY LNA miRNA Custom PCR Panels
Custom 96- and 384-well plates of LNA-enhanced, SYBR® Green-based miRNA qPCR assays
Certal Residual DNA Detection Kits
Certal Residual DNA Detection Kits
For sensitive probe-based detection of residual CHO host cell DNA, including an internal control
Taq PCR Master Mix Kit
Taq PCR Master Mix Kit
Taq PCR Master Mix Kit is a 2x solution of Taq DNA polymerase, dNTPs, MgCl2 and PCR buffer to simplify your workflow, reduce contamination risk and improve throughput and reproducibility
HotStarTaq Plus Master Mix Kit
HotStarTaq Plus Master Mix Kit
For fast and highly specific amplification in all applications
QuantiFast Multiplex PCR Kits
QuantiFast Multiplex PCR Kits
For fast, multiplex, real-time PCR and two-step qRT-PCR using sequence-specific probes
UltraRun LongRange PCR Kit
UltraRun LongRange PCR Kit
For ultrafast, hot-start mediated, long-range PCR
mericon Pathogen Detection Assay
mericon Pathogen Detection Assay
For real-time PCR detection of pathogen DNA in food or animal feed
QuantiNova Reverse Transcription Kit
QuantiNova Reverse Transcription Kit
For fast cDNA synthesis and reproducible real-time two-step RT-PCR
QIAGEN OneStep Ahead RT-PCR Kit
QIAGEN OneStep Ahead RT-PCR Kit
For faster one-step RT-PCR with high sensitivity, specificity and fidelity
miRCURY LNA miRNA Custom Probe PCR Assays
miRCURY LNA miRNA Custom Probe PCR Assays
For extremely sensitive and accurate quantification of mature miRNA using LNA-enhanced, probe-based qPCR
Microbial DNA qPCR Assays
Microbial DNA qPCR Assays
For real-time PCR-based microbial detection; includes one assay with Microbial qPCR Mastermix for 100 reactions
RT2 SYBR® Green FAST Mastermixes
RT2 SYBR® Green FAST Mastermixes
For use with 100-ring disc format RT2 Profiler and RT2 lncRNA PCR Arrays on the Rotor-Gene Q
qBiomarker Somatic Mutation PCR Arrays
qBiomarker Somatic Mutation PCR Arrays
For rapid and accurate profiling of the somatic DNA mutation status of gene panels
UCP Multiplex PCR Kit
UCP Multiplex PCR Kit
Ultra-Clean Production master mix for multiplex hot-start PCR and microbiome applications
qBiomarker Somatic Mutation PCR Assays
qBiomarker Somatic Mutation PCR Assays
For detecting the presence of specific DNA sequence mutations in cancer and oncogenesis
Microbial DNA qPCR Arrays
Microbial DNA qPCR Arrays
For real-time PCR-based, application-specific microbial identification or profiling
miRCURY LNA miRNA Probe PCR Assays
miRCURY LNA miRNA Probe PCR Assays
For extremely sensitive and accurate quantification of mature miRNA using LNA-enhanced, probe-based qPCR
miScript miRNA Mimics
miScript miRNA Mimics
For studies on miRNA function and gene regulation using synthetic miRNA
Type-it Mutation Detect PCR Kit
Type-it Mutation Detect PCR Kit
For accurate and reliable multiplex PCR analysis of mutations
QuantiNova LNA PCR Assays
QuantiNova LNA PCR Assays
For in-depth, accurate and reliable gene expression analysis using LNA-enhanced SYBR® Green qPCR
QuantiNova LNA PCR Assays for Digital PCR
QuantiNova LNA PCR Assays for Digital PCR
For in-depth, accurate and reliable gene expression analysis using LNA-enhanced, EvaGreen digital PCR
RT2 HT First Strand Kit
RT2 HT First Strand Kit
For cDNA synthesis and genomic DNA elimination in 96 RNA samples for use with RT2 Profiler PCR Arrays
miRCURY LNA Probe PCR Kits
miRCURY LNA Probe PCR Kits
For high-performance PCR with the miRCURY LNA miRNA Probe PCR System
FastLane Cell cDNA Kit
FastLane Cell cDNA Kit
For high-speed preparation of cDNA without RNA purification for use in real-time RT-PCR
QuantiTect Whole Transcriptome Kit
QuantiTect Whole Transcriptome Kit
For unlimited real-time PCR analysis from precious RNA samples
Real-time PCR Consumables
Real-time PCR Consumables
For optimal results in qPCR experiments
Microbial DNA qPCR Assay Kits
Microbial DNA qPCR Assay Kits
For real-time PCR-based microbial detection; includes one assay and everything required for a successful qPCR run for 20 reactions
QuantiNova LNA PCR Focus Panels
QuantiNova LNA PCR Focus Panels
For in-depth pathway- or disease-specific gene expression analysis using LNA-enhanced, SYBR® Green-based PCR
RT2 PreAMP cDNA Synthesis Kit
RT2 PreAMP cDNA Synthesis Kit
For preamplification of cDNA from small or FFPE samples of RNA for analysis on RT2 Profiler PCR Arrays
QuantiNova LNA Probe PCR Custom Assays
QuantiNova LNA Probe PCR Custom Assays
For in-depth, accurate and reliable gene expression analysis using LNA-enhanced hydrolysis probe-based PCR
XpressRef Universal Total RNA
XpressRef Universal Total RNA
For use as a positive control for gene expression detection methods
QuantiNova LNA Probe PCR Assays
QuantiNova LNA Probe PCR Assays
For in-depth, accurate and reliable gene expression analysis using LNA-enhanced hydrolysis probe-based PCR
RT2 lncRNA PCR Arrays
RT2 lncRNA PCR Arrays
For lncRNA expression analysis by pathway and disease using laboratory-verified SYBR® Green qPCR assays
QuantiNova LNA PCR Flexible Panels
QuantiNova LNA PCR Flexible Panels
For in-depth pathway- or disease-specific gene expression analysis using LNA-enhanced, SYBR® Green-based PCR
Microbial DNA Positive Control
Microbial DNA Positive Control
For use as a positive control for Microbial DNA qPCR Assays
RT2 Easy First Strand Kit
RT2 Easy First Strand Kit
For cDNA synthesis and genomic DNA elimination in RNA samples for use with RT2 Profiler PCR Arrays
RT2 lncRNA qPCR Assay
RT2 lncRNA qPCR Assay
For long noncoding gene expression analysis by real-time PCR
QuantiFast Probe PCR Kits
QuantiFast Probe PCR Kits
For fast, real-time PCR and two-step qRT-PCR using sequence-specific probes
QuantiNova LNA PCR Custom Assays for Digital PCR
QuantiNova LNA PCR Custom Assays for Digital PCR
For in-depth, accurate and reliable gene expression analysis using LNA-enhanced, EvaGreen digital PCR.
Rotor-Gene SYBR® Green PCR Demo Kit
Rotor-Gene SYBR® Green PCR Demo Kit
For evaluation of Rotor-Gene Q performance
RNA QC PCR Arrays
RNA QC PCR Arrays
For quality assessment of RNA samples before characterization using PCR arrays
RT2 qPCR Primer gDNA Controls
RT2 qPCR Primer gDNA Controls
For detection of genomic DNA contamination in RNA and cDNA
Supplemental Microbial qPCR Mastermix (ROX)
Supplemental Microbial qPCR Mastermix (ROX)
For real-time PCR applications
RT2 PreAMP Pathway Primer Mixes
RT2 PreAMP Pathway Primer Mixes
For amplification of cDNA templates prior to analysis on RT² Profiler PCR Arrays
QuantiNova LNA PCR Custom Panels
QuantiNova LNA PCR Custom Panels
For in-depth pathway- or disease-specific gene expression analysis using LNA-enhanced, SYBR® Green-based PCR
Custom RT2 PreAMP Pathway Primer Mixes
Custom RT2 PreAMP Pathway Primer Mixes
For amplification of cDNA templates prior to analysis on Custom RT² Profiler PCR Arrays
QuantiNova LNA Probe PCR Focus Panels
QuantiNova LNA Probe PCR Focus Panels
For in-depth pathway- or disease-specific gene expression analysis using LNA-enhanced, probe-based PCR
RT2 lncRNA PreAMP Primer Mixes
RT2 lncRNA PreAMP Primer Mixes
For cDNA synthesis prior to real-time PCR
Quantinova LNA PCR lncRNA Focus Panels
Quantinova LNA PCR lncRNA Focus Panels
For accurate and reliable lncRNA quantification using LNA-enhanced, SYBR® Green-based PCR
QuantiNova LNA Probe PCR lncRNA Focus Panels
QuantiNova LNA Probe PCR lncRNA Focus Panels
For accurate and reliable lncRNA quantification using LNA-enhanced, probe-based PCR
QuantiNova LNA Probe PCR Flexible Panels
QuantiNova LNA Probe PCR Flexible Panels
For in-depth pathway- or disease-specific gene expression analysis using LNA-enhanced, probe-based PCR
QuantiNova LNA Probe PCR Custom Panels
QuantiNova LNA Probe PCR Custom Panels
For in-depth pathway- or disease-specific gene expression analysis using LNA-enhanced, probe-based PCR
Supplemental Microbial qPCR Mastermix (Fluorescein)
Supplemental Microbial qPCR Mastermix (Fluorescein)
For real-time PCR applications
Housekeeping Genes
Housekeeping Genes
For use in normalizing your relative gene expression profiling experiment
RT2 Profiler PCR Array Modification
RT2 Profiler PCR Array Modification
For gene expression profiling using a partially modified pathway or disease panel
Viral Vector Direct Lysis Kits
Viral Vector Direct Lysis Kits
For the direct lysis of viral vectors, such as different adeno-associated virus (AAV) serotypes and adenoviruses and lentivirus (LV)
QIAprep&amp Viral RNA UM Kit
QIAprep&amp Viral RNA UM Kit
For rapid and high-frequency detection of SARS-CoV-2 by quantitative real-time PCR for epidemiological research

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