QIAshredder

For simple and rapid homogenization of cell and tissue lysates
  • Replaces syringe-and-needle homogenization
  • Reduces loss of sample material
  • Eliminates cross-contamination between samples
  • Filters out insoluble debris and reduces viscosity
The QIAshredder homogenizer consists of a unique biopolymer-shredding system in a microcentrifuge spin-column format. Cell or tissue lysate is loaded onto the QIAshredder homogenizer placed in a collection tube and centrifuged. The homogenized lysate is then collected. In general, similar yields and quality of RNA are obtained as with rotor–stator homogenization (see figure "Comparison of RNA yields").
Produto Num Cat. Preço de lista:
QIAshredder (250)
250 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps
79656
Cotação
QIAshredder (50)
50 disposable cell-lysate homogenizers for use in nucleic acid minipreps, caps
79654
Cotação
The QIAshredder is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

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Kit Handbooks
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RNeasy Mini Kit - For purification of total RNA from animal cells, animal tissues, bacteria, and yeast, and for RNA cleanup; RNeasy Protect Mini Kit - For immediate stabilization of RNA in harvested animal tissues and subsequent total RNA purification; RNeasy Plant Mini Kit - For purification of total RNA from plants and filamentous fungi
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Imagens
Comparison of RNA yields with different homogenization methods

Comparison of RNA yields.

Northern blot of total RNA isolated from 5 x 106 HeLa cells using the RNeasy Mini procedure with the indicated homogenization methods. RNA was eluted with 2 x 40 µl water, and 10 µl was loaded per lane.