Multi-Analyte ELISArray Kits follow a traditional ELISA protocol that can be completed in any laboratory with a microplate reader.
Each Multi-Analyte ELISArray contains twelve 8-well strips (represented by colored columns) coated with a specific capture antibody for a cytokine or chemokine. Six samples can be analyzed at one time, in addition to controls.
Th1/Th2 cytokines were induced by human peripheral blood mononuclear cells in response to PMA (50 µg/ml) and ionomycin (1 µg/ml). Time-dependent patterns of induced cytokines and relative cytokine amounts were profiled and plotted.
Similar standard curves are observed for all optimized cytokine and chemokine assays in the Multi-Analyte ELISArray Kits. All standard curves are virtually superimposable indicating that all 12 assays provide similar linearity and sensitivity under the same standardized conditions and development or incubation times.