Detection of SSA4 RNA in ∆rip1 fixed yeast cells using a single-labeled Cy3 Custom LNA mRNA Detection Probe (left) or a single-labeled Cy3 DNA oligonucleotide (right) of same length. The results show improved signal and less background staining with the Custom LNA mRNA Detection Probe. Pictures were taken after 20 min hybridization time in 50% formamide. From Thomsen et al., RNA, Nov 2005; 11(11): 1745–1748; reprinted by permission of CSHL Press.
Detection of a poly(A)+ RNA in fixed wild-type yeast cells using a single-labeled Cy3 T 20 Custom LNA mRNA Detection Probe (left) at 37ºC shows a nuclear signal that could not be detected using a single-labeled Cy3 T 70 DNA oligonucleotide (right). Both probes have identical Tm. Pictures were taken after 30 min hybridization time. From Thomsen et al., RNA, Nov 2005; 11(11): 1745–1748; reprinted by permission of CSHL Press.
Fixed Rat 9G cell hybridized with an HCMV-IE mRNA-specific Custom LNA mRNA Detection Probe. The bright red dot in the nucleus (stained blue with DAPI) represents the transcription and possibly RNA processing site of HCMV-IE mRNA. The mRNA is also detectable in the cytoplasm as red staining. Image kindly provided by Dr. R. W. Dirks, Leiden University, Leiden, The Netherlands.
The intensities of four probes
targeting rRNA from E. coli were compared. All probes had the same 19 nucleotide sequence, but the Custom LNA mRNA Detection Probes
had 1–3 LNA subsitutions. The LNA Probe 3 produced 22 times higher intensity than the DNA probe. Data from Kubota et al. Appl. Environ. Microbiol. 2006. 72: 5311–7.
