Average automated allele call rates for a panel of different DNAs were analyzed using 6 different TaqMan® MGB-based SNP genotyping assays and 1 ng of genomic DNA from each of the 60 samples. PCR was performed with the indicated products in 384-well plates in a 5 μl reaction volume. Allelic discrimination plate read was performed on an Applied Biosystems 7900HT instrument. The Type-it Fast SNP Probe PCR Kit consistently resulted in the highest call rates and the lowest error rates.

Buffer systems from other suppliers used for SNP genotyping often show poorer separation of allele clusters due to nonspecific binding of the mismatch probe. The Type-it Fast SNP Probe Buffer System provides increased discrimination by narrowing the probe melting temperature window, thereby reducing nonspecific binding of the mismatch probe. Wide and clear separation of allele clusters is obtained.