Reproducible automated purification.
Reproducible automated purification.
Purification at different expression levels.
Purification at different expression levels.
Reproducible automated purification. The indicated proteins were purified in duplicate under native conditions using Ni-NTA Spin Columns from cleared E. coli cell lysates derived from 5 ml LB cultures either manually or in an automated procedure on the QIAcube. CAT: chloramphenicol acetyl transferase; GFP: Green fluorescent protein; HIV-RT: Human immunodeficiency virus reverse transcriptase; IL-1b: Interleukin-1 beta. M: markers; C: cleared lysate (2 μl loaded per lane); E: elution fraction (3 μl loaded per lane).
Purification at different expression levels.

The 6xHis-tagged mouse DHFR was expressed at the indicated levels in E. coli, purified from 3 ml cultures using the Ni-NTA Spin Kit under denaturing conditions, and eluted in buffer at pH 5.9. Fractions were visualized by Coomassie staining after SDS-PAGE. 5 µl (of each 200 µl eluate) was loaded. 1: cell lysate; 2: flow-through 3: first eluate; 4: second eluate.