RGS·His HRP Conjugate Kit
For sensitive, direct detection of His-tagged proteins with RGS·His epitopes
Anti·His HRP Conjugates, including the RGS·His HRP Conjugate, are intended for sensitive detection of recombinant proteins carrying His tags, without the need for secondary antibodies. They consist of Anti·His antibodies conjugated to horseradish peroxidase (HRP). In detection procedures, the Anti·His antibody binds to a protein's His tag and the complex is localized using chemiluminescent or chromogenic substrates that react with HRP.
Anti·His HRP Conjugates consist of the QIAexpress Anti·His Antibodies chemically coupled to the reporter enzyme horseradish peroxidase (HRP). They can be used for highly specific and sensitive, direct detection of any recombinant protein that has an accessible His tag. They eliminate the need for secondary antibodies, saving time in immunodetection procedures. Anti·His HRP Conjugates can be used with chemiluminescent or chromogenic detection substrates.
The QIAexpress, Detection and Assay Handbook, supplied with all QIAexpress detection and assay products, contains detailed example protocols and guidelines for designing and performing assays.
Anti·His HRP Conjugates, including the RGS·His HRP Conjugate, recognize the same epitopes as their corresponding antibody and can be used for direct chemiluminescent or chromogenic detection of His-tagged proteins in:
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