qBiomarker Somatic Mutation PCR Assays

For detecting the presence of specific DNA sequence mutations in cancer and oncogenesis

Simple real-time PCR procedure
High sensitivity and wide dynamic range
Designed for routine use on most qPCR instruments
Master mix is included

qBiomarker Somatic Mutation PCR Assays identify the presence of individual specific sequence mutations present in cell lines or research samples that are critical for toxicological, drug development, and cancer studies. The mutations are selected from comprehensive curated databases (e.g., COSMIC) and literature reviews based on their clinical or functional relevance and frequency in patient populations.

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Cat No./ID: 337011

qBiomarker Somatic Mutation PCR Assays

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PCR assay and master mix

qBiomarker Somatic Mutation PCR Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.

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Principle

Real-time PCR is the most sensitive and reliable method for the detection of DNA mutations. By combining allele-specific amplification and hydrolysis probe detection, qBiomarker Somatic Mutation real-time PCR assays have been developed which can detect as few as 1% somatic mutations in the background of wild-type genomic DNA. Allele-specific amplification is achieved by Amplification Refractory Mutation System (ARMS) technology, which is based on the discrimination by Taq polymerase between a match and a mismatch at the 3’ end of the PCR primer (see figure "Principle of ARMS technology").

Procedure

After the isolation of genomic DNA from fresh, frozen, or fixed samples, add an aliquot of each sample to a separate real-time PCR tube containing the appropriate master mix (included) and the qBiomarker Somatic Mutation PCR Assay. Then, add an aliquot of each sample to a separate real-time PCR tube containing the appropriate master mix (included) and the corresponding reference gene copy assay. Finally, run the recommended cycling program.

Determine the C_T values for each mutation-specific assay and the corresponding reference gene copy assay for each sample using your instrument's software. Then, paste the values into the correct Excel-based data analysis template to determine which samples contain the tested mutation.

Applications

qBiomarker Somatic Mutation PCR Assays are highly suited for the rapid and accurate identification of individual specific sequence mutations present in fresh, frozen, or fixed samples.

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Application Notes (1)

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	High-quality genomic DNA isolation and sensitive mutation analysis	Show details

FAQs (20)

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	How many different genes and gene mutations are on a Somatic Mutation PCR Array? FAQ ID -2427	View
	How are the different mutations for a single gene normalized on the qBiomarker Somatic Mutation PCR array? FAQ ID -2428	View
	Where are the somatic mutations published? FAQ ID -2429	View
	How can I check DNA sample quality before using the Somatic Mutation PCR Arrays & Assays? FAQ ID -2430	View
	How much DNA starting sample is needed for analysis with a Somatic Mutations PCR Array? And Assay? FAQ ID -2431	View
	What is the sensitivity of the assays contained on the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2910	View
	How many mutations can I profile per sample on the qBiomarker Somatic Mutation PCR Array? FAQ ID -2911	View
	Were all assays on the qBiomarker Somatic Mutation PCR Arrays bench-validated? FAQ ID -2912	View
	How do you decide on the qBiomarker Somatic Mutation PCR Arrays to include on the pathway arrays and disease arrays? FAQ ID -2913	View
	What are the control features on the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2914	View
	What is the data analysis method, and where can I find it for the qBiomarker Somatic Mutatation PCR arrays? FAQ ID -2915	View
	What is the principle for qBiomarker Somatic Mutation PCR Array data analysis? FAQ ID -2916	View
	What PCR machine do I need to use to run such a qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2917	View
	What effects can be predicted with poor sample quality? How robust is the platform with questionable sample quality (such as FFPE samples)? FAQ ID -2918	View
	What sample types can I test on the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2919	View
	What method(s) do you recommend as an alternative way to validate the mutations identified on the somatic mutation PCR arrays? FAQ ID -2920	View
	What are the advantages of RT-PCR based somatic mutation PCR arrays and assays compared to other platforms? FAQ ID -2921	View
	What pathways and diseases are currently covered for the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2922	View
	How do I use the qBiomarker Somatic Mutation PCR Arrays? FAQ ID -2923	View
	What are the technological principles behind somatic mutation PCR arrays and assays? FAQ ID -2924	View

White Papers (1)

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	(EN) - Rapid and accurate cancer somatic mutation profiling with the qBiomarker Somatic Mutation PCR Arrays	Show details

Instrument Technical Documents (2)

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	(EN) - qBiomarker Somatic Mutation PCR Arrays For screening disease-focused mutation panels by PCR	Show details
	QIAGEN Service Core - (EN) For gene expression and genomic analysis	Show details

Performance Data (3)

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	qBiomarker Human DNA QC PCR Array	Show details
	qBiomarker Somatic Mutation PCR Array 384HT	Show details
	qBiomarker Somatic Mutation PCR Array	Show details

Kit Handbooks (1)

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	qBiomarker Somatic Mutation PCR Handbook For real-time PCR-based, pathway- or disease-focused somatic mutation profiling	Show details

Safety Data Sheets (1)

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	MSDS qBiomarker Somatic Mutation PCR Assays