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Cat. No. / ID: 931436
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
The QIAsymphony DNA Investigator Kit enables automated purification of genomic DNA from 1–96 samples, such as swabs, filters, casework or crime-scene samples, and blood on the QIAsymphony SP. Purification is fast and efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis, with high signal-to-noise ratios.
The QIAsymphony DNA Investigator Kit enables highly efficient purification of DNA, even from very small amounts of sample material. The combination of sample input volumes of up to 1000 µl with elution volumes as low as 30 µl enables highly sensitive detection of DNA from a wide range of sample types, including diffuse stains.
The purification procedure is designed to ensure safe and reproducible handling of precious or potentially infectious samples, and comprises 4 steps: lyse, bind, wash and elute. The user can choose different elution volumes between 30 μl to 400 μl of water or modified TE Buffer (Buffer ATE), depending on the protocol.
Since the type of samples that can be processed using the QIAsymphony DNA Investigator Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions in the presence of proteinase K and Buffer ATL in total volumes of either 200 μl, 500 μl or 1000 μl.
There are two different kinds of DNA purification protocols, which can be used in conjunction with the pretreatment protocols. Casework protocols purify genomic and mitochondrial DNA from 200 μl, 500 μl or 1000 μl of lysate obtained from the sample lysis procedures detailed in the corresponding pretreatment protocols. DNA can be eluted in 30–200 μl of either water or modified TE Buffer (Buffer ATE). Reference protocols purify DNA from database samples, such as buccal swabs or dried blood. DNA can be eluted in 100–400 μl of Buffer ATE. The purified DNA is ready to use in downstream applications.
| Protocol | Lysis volume options | Enhanced binding | Eluate volume |
|---|---|---|---|
| Reference | 200, 500 μl | No | 100–400 μl |
| Casework | 200, 500, 1000 μl | No | 100–200 μl |
| Casework ADV | 200, 500, 1000 μl | Yes | 100–200 μl |
| Casework HE | 200, 500, 1000 μl | No | 30–80 μl |
| Casework HE ADV | 200, 500, 1000 μl | Yes | 30–80 μl |
The high-quality DNA obtained using the QIAsymphony DNA Investigator Kit is suitable for direct use in forensic, environmental, biodefense, biosecurity, and other applied-testing applications, such as:
| Features | Specifications |
| Applications | STR analysis, (real-time) PCR |
| Yield | < 3 µg |
| Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein | Genomic DNA, mitochondrial DNA |
| Elution volume | Reference samples: 100-400 µl; Casework samples: 30- 200 µl |
| Sample type | Wide range of forensic and human-identity sample materials |
| Input volume | Reference samples: 200 or 500 µl lysate; Casework samples: 200, 500 or 1000 µl lysate |
| Technology | Magnetic particle technology |
