EZ2 AdnaTest CTC Select provides prefilled cartridges containing all buffers for the enrichment and lysis of CTCs. A fresh blood sample is incubated with CTC Select beads before loading the sample into the EZ2 Connect instrument. The instrument automatically pierces the prefilled cartridges and enriches the tumor cells using in-tip magnetic bead separation. AdnaTest CTC Select enables the immunomagnetic enrichment of tumor cells from whole blood via epithelial- and tumor-associated antigens. Antibodies against epithelial- and tumor-associated antigens are conjugated to magnetic beads for labeling of tumor cells. Labeled cells are extracted by the EZ2 Connect and lysed. The cartridges also contain oligo (dT) 25-coated beads for isolation of mRNA from the lysate of pre-enriched tumor cells (see figure " During the run"). Reverse transcription results in cDNA that can be used as template for tumor cell detection and characterization by PCR.
Importantly, the AdnaTest is an open platform that allows the profiling of additional custom-selected mRNA targets for comprehensive biomarker discovery.