PyroMark Supplementary Software

For expanded assay setup and analysis with PyroMark systems

Features

  • User-friendly interface increases the range of analyses available
  • Driven by drop-down menus to ensure correct selection of parameters
  • Enable user-designed assays with algorithms for optimization of primers

Product Details

PyroMark supplementary software expands assay flexibility and analysis breadth of Pyrosequencing analysis. Intuitive and user-friendly, these software products give the user access to optimized assay design for genotyping, allele quantification, methylation analysis, and de novo sequencing, as well as a framework for sequence comparison with entries in a local sequence database.

Principle

PyroMark Assay Design SW 2.0
The flexible PyroMark Assay Design Software 2.0 optimizes Pyrosequencing analyses. Featuring algorithms for full quality control and smooth import of assays into any PyroMark instrument, this software was constructed based on years of expertise in Pyrosequencing and PCR optimization. With a few clicks, the software designs PCR and sequencing primers for genotyping, allele quantification, sequence analysis, and methylation analysis. For methylation analysis, the software anticipates the bisulfite converted sequence and highlights CpG sites and non-CpG cytosines that can serve as controls.
 
PyroMark CpG SW 1.0
When analyzing multiple samples, each potentially with several methylation sites, it is important to have the aid of software that enables visualization of sequence-wide patterns, as well as details of specific variable positions. CpG analysis with PyroMark CpG SW 1.0 for the PyroMark Q96 ID and PyroMark Q96 MD provide that functionality. Standard calculations include methylation frequency, quality assessment, mean methylation values per well and replicates, statistical methylation patterns of multiple consecutive sites, and deviation from expected methylation patterns. Results are displayed in simple-to-understand graphical and numerical representations.
 
PyroMark IdentiFire SW 1.0
PyroMark IdentiFire SW 1.0 facilitates the compilation of a local sequence database, against which Pyrosequencing output are rapidly matched. The raw data, matched hits, and percent concordance of each hit are presented in detailed identification reports. This software is designed to interface directly with the PyroMark Q96 ID, but it also accepts run files imported from the PyroMark Q24.

Resources

Safety Data Sheets (1)
Download Safety Data Sheets for QIAGEN product components.
Scientific Posters (1)
Operating Software (1)
PyroMark Assay Design Software version 2.0.2 is compatible with Windows 7 and Windows 10 (64 bit) operating systems. This software may only be downloaded by registered users with a valid PyroMark Assay Design Software license. If you do not have a valid software license, contact your QIAGEN sales representative.

FAQ

Which analyses can be performed with the PyroMark Q96 ID software version 1.0?
The PyroMark Q96 ID software version 1.0 contains a SNP mode for simplex and multiplex entries (and Allele Quantification analyses can be performed for simplex entries) and an SQA mode for de novo sequencing analysis. This software version does not contain a CpG mode for methylation analysis, however the PyroMark CpG software v1.0 can be used.
FAQ ID -2845
Which analyses can be performed with the PyroMark Q96 MD software?

The PyroMark Q96 MD software contains a SNP mode for simplex and multiplex entries (and Allele Quantification analyses can be performed for simplex entries). This software version does not contain a CpG mode for methylation analysis, however the PyroMark CpG software v1.0 can be used. SQA analysis for de novo sequencing is not possible. 

See our Product Selection Guide for additional information on CpG supplementary software.

FAQ ID -2866
What are the changes of PyroMark Assay Design Software Version 2.0 compared to 1.0?
The new PyroMark Assay Design Software Version 2.0 contains new algorithms to facilitate CpG assay design and enables PCR and sequencing primer design for all pyroseqeuncing applications and PyroMark instruments.
FAQ ID -2849