artus Enterovirus LC RT-PCR Kit RUO

The artus Enterovirus LC RT-PCR Kit is a ready-to-use molecular detection kit for real-time PCR. The kit provides all necessary reagents optimized for reliable enterovirus RNA detection and quantitation.

To ensure sensitivity, the artus Enterovirus LC RT-PCR Kit has been optimized to detect low numbers of enterovirus RNA. The analytical sensitivity of the kit is 3.2 copies/µl in the PCR.

The artus Enterovirus LC RT-PCR Kit is based on the amplification and simultaneous detection of a specific region of the enterovirus genome using real-time PCR. The kit provides high levels of specificity and sensitivity.

Each artus Enterovirus LC RT-PCR Kit provides 4 enterovirus quantitation standards (see figure " Reliable quantitation of enterovirus load". Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical enterovirus RT-PCR is not reduced.

The artus Enterovirus LC RT-PCR Kit provides all necessary reagents optimized for reliable enterovirus RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For RNA purification, we recommend using the QIAamp Viral RNA Mini Kit.

The artus Enterovirus LC RT-PCR Kit enables rapid and sensitive detection of enterovirus RNA from cerebrospinal fluid (CSF) with accurate quantification. The kit is available for use on the Lightcycler 1.1/1.2/1.5 Instruments.