Viral nucleic acids were purified from 200 µl plasma samples spiked with 10,000, 1000 and 100 IU/ml of a typical DNA or RNA virus. Sample processing was automated using either QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol, QIAxtractor with the VX Protocol, or QIAcube with the QIAamp MinElute Virus Spin Kit. Viral nucleic acids were detected using in-house PCR and RT-PCR assays, with 20 µl eluate per reaction on the Rotor-Gene Q. Results show that QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit performs as well as or better than the other methods.
Various sample types were spiked with 20,000 IU of a typical DNA virus. Viral DNA was purified from 200 µl of each sample lysate using the QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol. Purified viral DNA was detected using an in-house PCR assay with 20 µl each eluate per reaction.
Nucleic acids were purified from serial dilutions of plasma samples spiked with a typical RNA or DNA virus. Samples were processed using QIAcube HT with the QIAamp 96 Virus QIAcube HT Kit and protocol and were analyzed using in-house PCR and RT-PCR assays. The percentage of positive samples at low virus titers is shown. The resulting 95% probit values were 316.84 IU/ml for the RNA virus and 18.54 IU/ml for the DNA virus.