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Cignal Reporter Assay Kits

For rapid, sensitive, and quantitative assessment of signal transduction pathway activation

  • Rapid analysis of signal transduction pathway regulation
  • Exceptional sensitivity and specificity
  • Transfection-ready constructs
  • Includes positive and negative controls
  • Real-time live cell quantification (GFP format)

Cignal Reporter Assays provided in the Cignal Reporter Assay Kit enable rapid, sensitive, and quantitative assessment of signal transduction pathway activation by measuring the activities of downstream transcription factors. Two reporter systems are available: dual-luciferase format and GFP format.

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Cignal Reporter Assay Kits are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.


0
TNFα activates NFkB signaling activity in a dose-dependent manner.
293 H cells were transfected with Cignal NFkB Reporter Assay, negative or positive control. After 24 hours, cells were treated with different doses of hTNFα for a further 24 hours. Dual-luciferase assays were performed, and reporter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate and standard deviation is shown.
1
Assessing RNA interference phenotypes.

Cignal p53 Reporter Assays showed that p53 siRNA treatment abolished p53 transcription activity. HCT 116 cells were transfected with p53 reporter, negative control, and positive control, together with p53 siRNA or negative control siRNA. Dual-luciferase assays were performed. Reporter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate and the standard deviation is shown.

2
Assessing overexpression phenotypes.

Cignal RBP-Jκ Reporter Assay showed upregulation of Notch signaling activity after overexpression of activated Notch1. 293 H cells were transfected with RBP-Jκ reporter, negative control, and positive control. After 24 hours, cells were infected with 100 MOI of recombinant adenoviruses expressing activated Notch1 (Ad-NICD) or 100 MOI of recombinant adenovirus expressing GFP (Ad-GFP) for another 18 hours. Dual-luciferase assays were performed. Reporter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate and the standard deviation is shown.

3
Verification of small molecule drug candidate.

Cignal Retinoic Acid Response Element (RARE) Reporter Assay reported elevated retinoic acid receptor pathway activity after the treatment of all trans-retinoic acid (ATRA). CHO-K1 cells were transfected with RARE reporter, negative control, and positive control. After 16 hours, medium was changed to assay medium. After 24 hours, cells were treated with 1 µM all trans-rectinoic acid (ATRA) for 6 hours. Dual-luciferase assay was performed, and promoter activity values are expressed as arbitrary units using a Renilla reporter for internal normalization. Experiments were performed in triplicate and the standard deviation is shown.

4
Cignal Reporter Assay shows that hTNFa activated NFkB signaling pathway.
293 H cells were transfected with the Cignal NFkB-GFP Reporter Assay or negative control. After 16 hours, medium was changed to assay medium. After 24 hours, cells were treated with 10 ng/ml TNFα. After 18 hours, medium was removed from the wells and fluorescence activity was measured using a fluorometer. The fluorescence activity present in treated and nontreated negative control wells was subtracted from the fluorescence activity in treated and nontreated Cignal NFkB-GFP Reporter Assay wells and relative fluorescence activities expressed as arbitrary units. Experiments were performed in triplicate and the standard deviations are shown.
5
Cignal Reporter Assay measures activation of serum response factor (SRF) transcription activity.

293 H cells were transfected with the Cignal SRF-GFP Reporter Assay or negative control. After 16 hours, medium was changed to assay medium. After 24 hours, cells were treated with 10 ng/ml PMA and 10% serum. After 18 hours, medium was removed from the wells and fluorescence activity was measured using a fluorometer. The fluorescence activity present in treated and nontreated negative control wells was subtracted from the fluorescence activity in treated and nontreated Cignal SRF-GFP Reporter Assay wells and relative fluorescence activities expressed as arbitrary units. Experiments were performed in triplicate and the standard deviations are shown.

6
Cignal Reporter Assay procedure.
7
Cignal Reporter Assay principle.
Performance
Principle

Cignal Reporter Assays consist of multiple repeats of a specific transcription factor’s binding site and basic promoter elements to drive the expression of a reporter gene (firefly luciferase or GFP). These reporters are powerful tools in functional genomics and drug discovery for assessing pathway activity. When the pathway is activated or inhibited by a drug candidate, gene knockdown, overexpression, or peptide, luciferase or GFP reporter activity is modulated and can be measured quantitatively and rapidly (see figure "Cignal Reporter Assay principle"). The Cignal Reporter Assays are available as transfection-ready constructs utilizing two reporter systems: the dual-luciferase reporter or the GFP reporter (see flowchart "Cignal Reporter Assay procedure").

The dual-luciferase format is an end-point assay providing unsurpassed sensitivity, specificity, and signal-to-noise ratios. This format is available for all Cignal Reporter Assays. Each assay includes a pathway-focused transcription factor reporter and a noninducible negative control, as well as luciferase and GFP positive controls.

The GFP format is an outstanding method for monitoring live cell pathway regulation, with single cell resolution. It includes a pathway-focused transcription factor reporter and positive and negative controls.

Procedure

Cignal Reporter Assays include a preformulated, transfection-ready pathway reporter (dual-luciferase or GFP), plus a positive and negative control. The inducible pathway reporter and noninducible negative control are transfected and subjected to experimental treatments in parallel.

Dual-luciferase

Dual-luciferase results are calculated for each transfectant. The change in the activity of each signaling pathway is determined by comparing the normalized luciferase activities of the reporter in treated versus untreated transfectants. The identically treated negative control transfectants serve as a specificity control. The positive control serves as a control for transfection efficiency, by monitoring firefly luciferase and Renilla expression.

GFP

GFP expression is quantified using a flow cytometer, fluorescence microscope, or fluorometer. The change in the activity of each signaling pathway is determined by comparing the GFP activities in treated versus untreated transfectants. The identically treated negative control transfectants serve as a specificity control. The positive control serves as a control for transfection efficiency, by monitoring GFP expression from the constitutively expressing CMV-GFP reporter.

Applications

Cignal Reporter Assays are powerful tools in functional genomics, proteomics, and drug discovery for assessing the biological impact of pathway inhibition or activation with siRNAs, proteins, and small molecule compounds.

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Brochures & Guides (1)
For cell-based analysis of pathway signaling activity
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Kit Handbooks (1)
Transfection Protocols (2)
Search for transfection data by nucleic acid, cell line, and transfection reagent. Our database contains data from researchers like yourself who have shared their experimental results with us.
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Transfection protocols for specific cell types and plate formats that save you the time and effort of adapting existing protocols to fit your requirements. Simply select the cell type, nucleic acid, and culture format to receive a QIAGEN transfection protocol to print out or download in convenient PDF format.
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GATA Reporter Assay Kit Cignal Reporter Assay Kits
Introduction
The Cignal GATA Reporter Assay Kit is designed to measure the transcriptional activity of globin transcription factor (GATA) family members. The GATA family of transcription factors contains six zinc-finger binding proteins that regulate differentiation and cell proliferation. GATA family members are involved in hematopoietic, cardiac and gut development. The GATA reporter is a mixture of a GATA-responsive luciferase construct and a constitutively expressing Renilla luciferase construct (40:1). The GATA-responsive luciferase construct encodes the firefly luciferase reporter gene under the control of a minimal (m)CMV promoter and tandem repeats of the GATA transcriptional response element. The number of response elements and the intervening sequence between these response elements has been experimentally optimized to maximize the signal to noise ratio. The GATA reporter construct monitors both increases and decreases in the transcriptional activity of GATA, and hence the activity of GATA signaling. The constitutively expressing Renilla luciferase construct encodes the Renilla luciferase reporter gene under the control of a CMV immediately early enhancer/promoter and acts as an internal control for normalizing transfection efficiencies and monitoring cell viability. Using a simple dual-luciferase assay, you can easily monitor the activity of GATA and determine the effect of various treatments, such as gene knockdown, over-expression, and chemical compounds.
CCS-1035L

The Cignal GATA Reporter Assay shows GATA transcription activity. HEK-293 cells were transfected with the Cignal GATA Reporter, negative control, and positive control (for the transfection protocol, refer to the Cignal Reporter Assay Handbook). After 16 hours of transfection, medium was changed to assay medium (Opti-MEM + 0.5% FBS + 0.1 mM NEAA + 1 mM sodium pyruvate + 100 U/ml penicillin + 100 µg/ml streptomycin). After 24 hours of transfection, cells were treated with 10 ng/ml PMA or an equal volume of DMSO for 18 hours. A dual-luciferase assay was performed 42 hours after transfection, and promoter activity values are expressed as arbitrary units using a Renilla luciferase reporter for internal normalization. Experiments were performed in triplicate, and the standard deviation is indicated.
Name Cignal GATA Reporter (luc) Kit (CCS-1035L)
Official symbol GATA1 [Human]
Official name GATA binding protein 1 (globin transcription factor 1)
Species Human (Homo sapiens)
Entrez Gene ID 2623
Transcript(s) for this gene NM_002049 (1501 bp), ENST00000376665, ENST00000376670
Official symbol Gata1 [Mouse]
Official name GATA binding protein 1
Species Mouse (Mus musculus)
Entrez Gene ID 14460
Transcript(s) for this gene NM_008089 (1902 bp), XM_011247448 (1951 bp)
Official symbol Gata1 [Rat]
Official name GATA binding protein 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 25172
Transcript(s) for this gene NM_012764 (1863 bp)
Official symbol IFNG [Human]
Official name interferon, gamma
Species Human (Homo sapiens)
Entrez Gene ID 3458
Transcript(s) for this gene NM_000619 (1240 bp), ENST00000229135
Official symbol Ifng [Mouse]
Official name interferon gamma
Species Mouse (Mus musculus)
Entrez Gene ID 15978
Transcript(s) for this gene K00083 (1192 bp), NM_008337 (1210 bp), XM_125899 (1207 bp)
Official symbol Ifng [Rat]
Official name interferon gamma
Species Rat (Rattus norvegicus)
Entrez Gene ID 25712
Transcript(s) for this gene NM_138880 (525 bp)
Official symbol IL5 [Human]
Official name interleukin 5
Species Human (Homo sapiens)
Entrez Gene ID 3567
Transcript(s) for this gene NM_000879 (816 bp), ENST00000462418, XM_005271988 (891 bp), XM_006714601 (1043 bp), ENST00000231454
Official symbol Il5 [Mouse]
Official name interleukin 5
Species Mouse (Mus musculus)
Entrez Gene ID 16191
Transcript(s) for this gene NM_010558 (1534 bp), XM_011248765 (3015 bp)
Official symbol Il5 [Rat]
Official name interleukin 5
Species Rat (Rattus norvegicus)
Entrez Gene ID 24497
Transcript(s) for this gene NM_021834 (399 bp)
Official symbol IL13 [Human]
Official name interleukin 13
Species Human (Homo sapiens)
Entrez Gene ID 3596
Transcript(s) for this gene NM_002188 (1282 bp), ENST00000487267, ENST00000468334, ENST00000304506, ENST00000617259
Official symbol Il13 [Mouse]
Official name interleukin 13
Species Mouse (Mus musculus)
Entrez Gene ID 16163
Transcript(s) for this gene NM_008355 (1217 bp)
Official symbol Il13 [Rat]
Official name interleukin 13
Species Rat (Rattus norvegicus)
Entrez Gene ID 116553
Transcript(s) for this gene NM_053828 (443 bp)
Official symbol NR5A2 [Human]
Official name nuclear receptor subfamily 5, group A, member 2
Species Human (Homo sapiens)
Entrez Gene ID 2494
Transcript(s) for this gene NM_003822 (4956 bp), NM_205860 (5094 bp), NM_001276464 (4787 bp), XM_005245061 (5119 bp), XM_005245062 (4695 bp), ENST00000236914, ENST00000367362, ENST00000544748
Official symbol Nr5a2 [Mouse]
Official name nuclear receptor subfamily 5, group A, member 2
Species Mouse (Mus musculus)
Entrez Gene ID 26424
Transcript(s) for this gene NM_001159769 (3635 bp), NM_030676 (3720 bp), XM_990082 (2016 bp), XM_006529620 (5235 bp), XM_006529621 (4620 bp), XM_006529622 (4670 bp)
Official symbol Nr5a2 [Rat]
Official name nuclear receptor subfamily 5, group A, member 2
Species Rat (Rattus norvegicus)
Entrez Gene ID 60349
Transcript(s) for this gene NM_021742 (1931 bp), XM_006249928 (4706 bp), XM_006249929 (4446 bp)
Official symbol LMO2 [Human]
Official name LIM domain only 2 (rhombotin-like 1)
Species Human (Homo sapiens)
Entrez Gene ID 4005
Transcript(s) for this gene NM_001142315 (1605 bp), NM_001142316 (1705 bp), NM_005574 (2303 bp), ENST00000493667, XM_005252920 (1665 bp), XM_005252921 (1800 bp), XM_006718229 (1875 bp), ENST00000257818, ENST00000395833
Official symbol Lmo2 [Mouse]
Official name LIM domain only 2
Species Mouse (Mus musculus)
Entrez Gene ID 16909
Transcript(s) for this gene NM_001142335 (1519 bp), NM_001142336 (1605 bp), NM_001142337 (1529 bp), NM_008505 (1646 bp), XM_001001351 (1643 bp), XM_001479884 (1643 bp), ENSMUST00000133210, XM_006498820 (1564 bp), XM_006498821 (1689 bp)
Official symbol Lmo2 [Rat]
Official name LIM domain only 2
Species Rat (Rattus norvegicus)
Entrez Gene ID 362176
Transcript(s) for this gene NM_001037358 (1639 bp), NM_001244779 (1529 bp), NM_001244780 (1504 bp), NM_001244781 (1534 bp), XM_342475 (716 bp)
Official symbol KLF1 [Human]
Official name Kruppel-like factor 1 (erythroid)
Species Human (Homo sapiens)
Entrez Gene ID 10661
Transcript(s) for this gene NM_006563 (1615 bp), ENST00000264834
Official symbol Klf1 [Mouse]
Official name Kruppel-like factor 1 (erythroid)
Species Mouse (Mus musculus)
Entrez Gene ID 16596
Transcript(s) for this gene NM_010635 (1534 bp), XM_987384 (1138 bp)
Official symbol Klf1 [Rat]
Official name Kruppel-like factor 1 (erythroid)
Species Rat (Rattus norvegicus)
Entrez Gene ID 304666
Transcript(s) for this gene NM_001107164 (1514 bp), XM_001071193 (1521 bp), XM_222473 (1521 bp), XM_006255254 (1621 bp)
Official symbol TFCP2 [Human]
Official name transcription factor CP2
Species Human (Homo sapiens)
Entrez Gene ID 7024
Transcript(s) for this gene NM_001173452 (3712 bp), NM_001173453 (3559 bp), NM_005653 (3715 bp), ENST00000546822, ENST00000257915, ENST00000548115, ENST00000549867
Official symbol Tfcp2 [Mouse]
Official name transcription factor CP2
Species Mouse (Mus musculus)
Entrez Gene ID 21422
Transcript(s) for this gene NM_033476 (3301 bp), NM_001289603 (3295 bp), XM_006520734 (1935 bp), XM_006520735 (1923 bp), XM_006520736 (1905 bp), XM_006520737 (3293 bp), XM_006520738 (3256 bp), XM_006520739 (3281 bp), XM_006520740 (1439 bp), XM_011245555 (3263 bp), XM_011245556 (2780 bp)
Official symbol BMP4 [Human]
Official name bone morphogenetic protein 4
Species Human (Homo sapiens)
Entrez Gene ID 652
Transcript(s) for this gene NM_001202 (1957 bp), NM_130850 (1748 bp), NM_130851 (1802 bp), ENST00000558489, XM_005268015 (1741 bp), ENST00000245451, ENST00000417573, ENST00000558984, ENST00000559087
Official symbol Bmp4 [Mouse]
Official name bone morphogenetic protein 4
Species Mouse (Mus musculus)
Entrez Gene ID 12159
Transcript(s) for this gene NM_007554 (2059 bp), ENSMUST00000135408, XM_006518467 (1843 bp), XM_006518468 (1902 bp), NM_001316360 (1934 bp), XM_011244934 (1736 bp), XM_011244935 (1699 bp)
Official symbol Bmp4 [Rat]
Official name bone morphogenetic protein 4
Species Rat (Rattus norvegicus)
Entrez Gene ID 25296
Transcript(s) for this gene NM_012827 (1559 bp), XM_006251746 (2453 bp), XM_006251747 (2003 bp), XM_006251748 (1985 bp), XM_006251749 (1727 bp), XM_006251751 (1704 bp), XM_008770501 (1817 bp)
Official symbol TAL1 [Human]
Official name T-cell acute lymphocytic leukemia 1
Species Human (Homo sapiens)
Entrez Gene ID 6886
Transcript(s) for this gene NM_003189 (4892 bp), ENST00000481091, NM_001287347 (4664 bp), NM_001290403 (4774 bp), NM_001290404 (4779 bp), NM_001290405 (4647 bp), NM_001290406 (4171 bp), XM_005271159 (4846 bp), XM_005271160 (4996 bp), XM_006710862 (5028 bp), XM_006710863 (4218 bp), ENST00000294339, ENST00000371884
Official symbol Tal1 [Mouse]
Official name T cell acute lymphocytic leukemia 1
Species Mouse (Mus musculus)
Entrez Gene ID 21349
Transcript(s) for this gene NM_011527 (4237 bp), NM_001287388 (4254 bp), XM_006502909 (4024 bp), XM_006502910 (4769 bp), XM_006502912 (4174 bp), XM_006502913 (4458 bp), XM_006502914 (4622 bp), XM_006502915 (4206 bp), XM_006502916 (4022 bp)
Official symbol Tal1 [Rat]
Official name TAL bHLH transcription factor 1, erythroid differentiation factor
Species Rat (Rattus norvegicus)
Entrez Gene ID 313507
Transcript(s) for this gene NM_001107958 (4225 bp), XM_001069740 (968 bp), XM_233430 (3130 bp), XM_006238615 (4091 bp), XM_006238617 (4299 bp), XM_006238618 (3943 bp), XM_008763967 (4610 bp)
Official symbol IL4 [Human]
Official name interleukin 4
Species Human (Homo sapiens)
Entrez Gene ID 3565
Transcript(s) for this gene NM_000589 (642 bp), NM_172348 (594 bp), ENST00000495905, ENST00000231449, ENST00000350025, ENST00000622422
Official symbol Il4 [Mouse]
Official name interleukin 4
Species Mouse (Mus musculus)
Entrez Gene ID 16189
Transcript(s) for this gene NM_021283 (605 bp), NR_027491 (624 bp)
Official symbol Il4 [Rat]
Official name interleukin 4
Species Rat (Rattus norvegicus)
Entrez Gene ID 287287
Transcript(s) for this gene NM_201270 (475 bp)
Official symbol PDGFRA [Human]
Official name platelet-derived growth factor receptor, alpha polypeptide
Species Human (Homo sapiens)
Entrez Gene ID 5156
Transcript(s) for this gene NM_006206 (6574 bp), ENST00000509092, XM_005265743 (6358 bp), XM_006714039 (6619 bp), XM_006714040 (6309 bp), XM_006714041 (3097 bp), ENST00000257290, ENST00000508170
Official symbol Pdgfra [Mouse]
Official name platelet derived growth factor receptor, alpha polypeptide
Species Mouse (Mus musculus)
Entrez Gene ID 18595
Transcript(s) for this gene NM_001083316 (6570 bp), NM_011058 (6553 bp), XM_006504261 (7148 bp), XM_006504262 (6608 bp), XM_006504263 (6636 bp)
Official symbol Pdgfra [Rat]
Official name platelet derived growth factor receptor, alpha polypeptide
Species Rat (Rattus norvegicus)
Entrez Gene ID 25267
Transcript(s) for this gene NM_012802 (6532 bp), XM_001067574 (5856 bp), XM_001067631 (5899 bp), XM_001067681 (5941 bp), XM_214030 (6518 bp), XM_006250879 (6570 bp), XM_006250880 (6876 bp), XM_006250881 (6524 bp)
Official symbol HNF4A [Human]
Official name hepatocyte nuclear factor 4, alpha
Species Human (Homo sapiens)
Entrez Gene ID 3172
Transcript(s) for this gene NM_000457 (4737 bp), NM_001030003 (4528 bp), NM_001030004 (1449 bp), NM_175914 (4558 bp), NM_178849 (4707 bp), NM_178850 (1628 bp), NM_001258355 (4828 bp), NM_001287182 (4750 bp), NM_001287183 (4780 bp), NM_001287184 (1671 bp), XM_005260407 (4817 bp), ENST00000316099, ENST00000316673, ENST00000415691, ENST00000443598, ENST00000457232, ENST00000609795, ENST00000619550
Official symbol Hnf4a [Mouse]
Official name hepatic nuclear factor 4, alpha
Species Mouse (Mus musculus)
Entrez Gene ID 15378
Transcript(s) for this gene NM_008261 (4391 bp), ENSMUST00000131658, XM_006498787 (4356 bp), XM_006498788 (4305 bp), NM_001312906 (4393 bp), NM_001312907 (4363 bp), XM_011239305 (4312 bp), XM_011239306 (4282 bp)
Official symbol Hnf4a [Rat]
Official name hepatocyte nuclear factor 4, alpha
Species Rat (Rattus norvegicus)
Entrez Gene ID 25735
Transcript(s) for this gene NM_022180 (4416 bp), NM_001270931 (4456 bp), NM_001270933 (4426 bp), XM_006235515 (2811 bp), XM_006235516 (2751 bp)
Official symbol GFI1B [Human]
Official name growth factor independent 1B transcription repressor
Species Human (Homo sapiens)
Entrez Gene ID 8328
Transcript(s) for this gene NM_001135031 (1663 bp), NM_004188 (2479 bp), XM_006717297 (1833 bp), XM_006717298 (2157 bp), ENST00000339463, ENST00000372122, ENST00000372123
Official symbol Gfi1b [Mouse]
Official name growth factor independent 1B
Species Mouse (Mus musculus)
Entrez Gene ID 14582
Transcript(s) for this gene NM_001160406 (1823 bp), NM_008114 (1724 bp), ENSMUST00000155686
Official symbol Gfi1b [Rat]
Official name growth factor independent 1B transcription repressor
Species Rat (Rattus norvegicus)
Entrez Gene ID 311832
Transcript(s) for this gene NM_001107823 (1618 bp), XM_001079155 (1686 bp), XM_231109 (1785 bp), XM_006233789 (1810 bp), XM_008761646 (1858 bp), XM_008761647 (5132 bp), XM_008761648 (2022 bp), XM_008761649 (1932 bp), XM_008761650 (2410 bp), XM_008761651 (1759 bp)
Official symbol ADH1A [Human]
Official name alcohol dehydrogenase 1A (class I), alpha polypeptide
Species Human (Homo sapiens)
Entrez Gene ID 124
Transcript(s) for this gene NM_000667 (1513 bp), ENST00000511656, ENST00000209668
Official symbol Zfpm1 [Mouse]
Official name zinc finger protein, multitype 1
Species Mouse (Mus musculus)
Entrez Gene ID 22761
Transcript(s) for this gene NM_009569 (3393 bp), XM_001001818 (3072 bp), ENSMUST00000177356, XM_006530851 (3762 bp)
Official symbol ZFPM1 [Human]
Official name zinc finger protein, FOG family member 1
Species Human (Homo sapiens)
Entrez Gene ID 161882
Transcript(s) for this gene NM_153813 (3241 bp), ENST00000569086, ENST00000319555, ENST00000562437, ENST00000563351, XM_011522912 (3359 bp), XM_011522913 (4647 bp), XM_011522914 (3333 bp), XM_011522915 (3185 bp), XM_011522916 (3187 bp), XM_011522917 (3078 bp), XM_011522918 (3365 bp)
Official symbol Spi1 [Mouse]
Official name spleen focus forming virus (SFFV) proviral integration oncogene
Species Mouse (Mus musculus)
Entrez Gene ID 20375
Transcript(s) for this gene NM_011355 (1333 bp), XM_001003437 (1019 bp), XR_104692 (613 bp), XR_107375 (617 bp), ENSMUST00000131400, XM_006499038 (1434 bp)
Official symbol SPI1 [Human]
Official name Spi-1 proto-oncogene
Species Human (Homo sapiens)
Entrez Gene ID 6688
Transcript(s) for this gene NM_001080547 (1426 bp), NM_003120 (1423 bp), ENST00000227163, ENST00000378538, ENST00000533030, ENST00000533968
Official symbol Spi1 [Rat]
Official name Spi-1 proto-oncogene
Species Rat (Rattus norvegicus)
Entrez Gene ID 366126
Transcript(s) for this gene NM_001005892 (1373 bp), XM_006234532 (2222 bp)
Official symbol Usf1 [Mouse]
Official name upstream transcription factor 1
Species Mouse (Mus musculus)
Entrez Gene ID 22278
Transcript(s) for this gene NM_009480 (1853 bp), ENSMUST00000161297, XM_006496747 (1796 bp), XM_006496748 (1860 bp), XM_006496749 (1765 bp), NM_001305676 (1799 bp), NM_001305677 (1863 bp), NM_001305678 (1768 bp)
Official symbol USF1 [Human]
Official name upstream transcription factor 1
Species Human (Homo sapiens)
Entrez Gene ID 7391
Transcript(s) for this gene NM_007122 (1830 bp), NM_207005 (1799 bp), NM_001276373 (1734 bp), ENST00000529476, ENST00000491629, ENST00000368019, ENST00000368020, ENST00000368021
Official symbol Usf1 [Rat]
Official name upstream transcription factor 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 83586
Transcript(s) for this gene NM_031777 (1720 bp), XM_006250266 (1870 bp), XM_008769765 (1521 bp)
Official symbol SULT2A1 [Human]
Official name sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
Species Human (Homo sapiens)
Entrez Gene ID 6822
Transcript(s) for this gene NM_003167 (1987 bp), ENST00000222002
Official symbol Sult2a1 [Rat]
Official name sulfotransferase family 2A member 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 24912
Transcript(s) for this gene NM_131903 (1000 bp), NM_133616 (981 bp), XM_008758891 (1790 bp), XM_008758892 (1036 bp), XM_008758893 (907 bp)
Official symbol Hdac3 [Mouse]
Official name histone deacetylase 3
Species Mouse (Mus musculus)
Entrez Gene ID 15183
Transcript(s) for this gene NM_010411 (2005 bp), ENSMUST00000153945, XR_385976 (0 bp)
Official symbol HDAC3 [Human]
Official name histone deacetylase 3
Species Human (Homo sapiens)
Entrez Gene ID 8841
Transcript(s) for this gene NM_003883 (1965 bp), ENST00000495485, ENST00000471968, ENST00000467533, XM_006714802 (932 bp), ENST00000305264
Official symbol Hdac3 [Rat]
Official name histone deacetylase 3
Species Rat (Rattus norvegicus)
Entrez Gene ID 84578
Transcript(s) for this gene NM_053448 (1799 bp)
Official symbol Esr1 [Mouse]
Official name estrogen receptor 1 (alpha)
Species Mouse (Mus musculus)
Entrez Gene ID 13982
Transcript(s) for this gene NM_007956 (6248 bp), XM_985634 (3048 bp), XM_985672 (6199 bp), XM_985704 (6760 bp), ENSMUST00000149195, XM_006512432 (6377 bp), XM_006512433 (6250 bp), XM_006512434 (6173 bp), XM_006512435 (6123 bp), XM_006512436 (2916 bp), NM_001302531 (6218 bp), NM_001302532 (6358 bp), NM_001302533 (2730 bp), XM_011243066 (6188 bp), XM_011243067 (6189 bp), XM_011243068 (6158 bp), XM_011243069 (1346 bp)
Official symbol ESR1 [Human]
Official name estrogen receptor 1
Species Human (Homo sapiens)
Entrez Gene ID 2099
Transcript(s) for this gene NM_000125 (6330 bp), NM_001122740 (6357 bp), NM_001122741 (6314 bp), NM_001122742 (6466 bp), ENST00000488573, NM_001291230 (6320 bp), NM_001291241 (6311 bp), XM_006715374 (6282 bp), XM_006715375 (5809 bp), ENST00000206249, ENST00000338799, ENST00000406599, ENST00000427531, ENST00000440973, ENST00000443427, ENST00000456483
Official symbol Esr1 [Rat]
Official name estrogen receptor 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 24890
Transcript(s) for this gene NM_012689 (2090 bp), XM_006227832 (6792 bp), XM_008758703 (6840 bp), XM_008758704 (6764 bp)
Official symbol Jarid2 [Mouse]
Official name jumonji, AT rich interactive domain 2
Species Mouse (Mus musculus)
Entrez Gene ID 16468
Transcript(s) for this gene NM_001205043 (5997 bp), NM_001205044 (5670 bp), NM_021878 (5716 bp), ENSMUST00000174683, XM_006516855 (5641 bp), XM_006516856 (6141 bp), XM_006516857 (5552 bp), XM_006516858 (5549 bp), XM_006516859 (5870 bp), XM_006516860 (5227 bp)
Official symbol JARID2 [Human]
Official name jumonji, AT rich interactive domain 2
Species Human (Homo sapiens)
Entrez Gene ID 3720
Transcript(s) for this gene NM_004973 (6112 bp), NM_001267040 (5612 bp), ENST00000474854, XM_005249089 (5415 bp), ENST00000341776, ENST00000397311
Official symbol Bin1 [Mouse]
Official name bridging integrator 1
Species Mouse (Mus musculus)
Entrez Gene ID 30948
Transcript(s) for this gene NM_001083334 (2135 bp), NM_009668 (2468 bp), XM_006525978 (2555 bp), XM_006525979 (2345 bp), XM_006525980 (2460 bp), XM_006525981 (2444 bp), XM_006525982 (2411 bp), XM_006525983 (2394 bp), XM_006525984 (2310 bp), XM_006525985 (2238 bp), XM_006525986 (2215 bp), XM_006525987 (2186 bp), XM_006525988 (2180 bp), XM_006525989 (2127 bp), XM_006525990 (2107 bp), XM_006525991 (2084 bp), XM_006525992 (2053 bp), XM_006525993 (2030 bp), XM_006525994 (2011 bp), XM_006525995 (1957 bp)
Official symbol BIN1 [Human]
Official name bridging integrator 1
Species Human (Homo sapiens)
Entrez Gene ID 274
Transcript(s) for this gene NM_004305 (2269 bp), NM_139343 (2686 bp), NM_139344 (2557 bp), NM_139345 (2425 bp), NM_139346 (2398 bp), NM_139347 (2461 bp), NM_139348 (2353 bp), NM_139349 (2332 bp), NM_139350 (2224 bp), NM_139351 (2134 bp), ENST00000484253, ENST00000466111, XM_005263642 (2783 bp), XM_005263643 (2690 bp), XM_005263644 (2645 bp), XM_005263645 (2174 bp), XM_005263646 (2369 bp), XM_005263647 (2231 bp), XM_005263648 (1670 bp), XM_006712424 (2597 bp), XM_006712425 (2651 bp), XM_006712426 (2651 bp), XM_006712427 (2129 bp), XM_006712428 (2543 bp), XM_006712429 (2522 bp), XM_006712430 (2498 bp), XM_006712431 (2453 bp), XM_006712432 (2414 bp), XM_006712433 (2324 bp), XM_006712434 (2279 bp), ENST00000259238, ENST00000316724, ENST00000346226, ENST00000348750, ENST00000351659, ENST00000352848, ENST00000357970, ENST00000376113, ENST00000393040, ENST00000393041, ENST00000409400, NM_001320641 (2593 bp), NM_001320642 (3180 bp), XM_011510975 (3410 bp)
Official symbol Bin1 [Rat]
Official name bridging integrator 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 117028
Transcript(s) for this gene NM_053959 (2399 bp), XM_006254496 (2518 bp), XM_006254497 (2377 bp), XM_006254498 (2344 bp), XM_006254499 (2278 bp), XM_006254500 (2206 bp), XM_006254501 (2182 bp), XM_006254502 (2149 bp), XM_006254503 (2137 bp), XM_006254504 (2008 bp), XM_006254505 (1936 bp), XM_008772012 (2212 bp), XM_008772013 (2116 bp), XM_008772014 (2053 bp)
Official symbol Foxa1 [Mouse]
Official name forkhead box A1
Species Mouse (Mus musculus)
Entrez Gene ID 15375
Transcript(s) for this gene NM_008259 (3188 bp), XM_994257 (2811 bp), XM_006515479 (3259 bp), XM_006515480 (3236 bp), XM_006515481 (3265 bp), XM_006515482 (3198 bp), XM_006515483 (3312 bp)
Official symbol FOXA1 [Human]
Official name forkhead box A1
Species Human (Homo sapiens)
Entrez Gene ID 3169
Transcript(s) for this gene NM_004496 (3396 bp), ENST00000557418, ENST00000250448
Official symbol Foxa1 [Rat]
Official name forkhead box A1
Species Rat (Rattus norvegicus)
Entrez Gene ID 25098
Transcript(s) for this gene NM_012742 (2189 bp)
Official symbol Hey1 [Mouse]
Official name hairy/enhancer-of-split related with YRPW motif 1
Species Mouse (Mus musculus)
Entrez Gene ID 15213
Transcript(s) for this gene NM_010423 (2215 bp), XM_006530053 (1915 bp)
Official symbol HEY1 [Human]
Official name hes related family bHLH transcription factor with YRPW motif 1
Species Human (Homo sapiens)
Entrez Gene ID 23462
Transcript(s) for this gene NM_001040708 (2331 bp), NM_012258 (2319 bp), ENST00000523531, NM_001282851 (2052 bp), ENST00000337919, ENST00000354724, ENST00000523976
Official symbol Hey1 [Rat]
Official name hes-related family bHLH transcription factor with YRPW motif 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 155437
Transcript(s) for this gene NM_001191845 (939 bp), XM_001057389 (948 bp), XM_342216 (939 bp)
Official symbol Zfpm2 [Mouse]
Official name zinc finger protein, multitype 2
Species Mouse (Mus musculus)
Entrez Gene ID 22762
Transcript(s) for this gene NM_011766 (4974 bp), XM_006520897 (4336 bp)
Official symbol ZFPM2 [Human]
Official name zinc finger protein, FOG family member 2
Species Human (Homo sapiens)
Entrez Gene ID 23414
Transcript(s) for this gene NM_012082 (4507 bp), ENST00000524235, XM_005250850 (4502 bp), ENST00000407775, ENST00000517361, ENST00000520492
Official symbol Zfpm2 [Rat]
Official name zinc finger protein, multitype 2
Species Rat (Rattus norvegicus)
Entrez Gene ID 314930
Transcript(s) for this gene NM_001130501 (4985 bp), XM_001062676 (4976 bp), XM_235253 (4976 bp)
Official symbol Gata6 [Rat]
Official name GATA binding protein 6
Species Rat (Rattus norvegicus)
Entrez Gene ID 29300
Transcript(s) for this gene NM_019185 (3044 bp), XM_001072268 (3041 bp)
Official symbol Stat3 [Rat]
Official name signal transducer and activator of transcription 3
Species Rat (Rattus norvegicus)
Entrez Gene ID 25125
Transcript(s) for this gene NM_012747 (4457 bp), XM_006247257 (4456 bp), XM_006247258 (4409 bp), XM_006247259 (4406 bp)
Official symbol STAT3 [Human]
Official name signal transducer and activator of transcription 3 (acute-phase response factor)
Species Human (Homo sapiens)
Entrez Gene ID 6774
Transcript(s) for this gene NM_003150 (4953 bp), NM_139276 (4978 bp), NM_213662 (4819 bp), ENST00000590776, XM_005257616 (5018 bp), XM_005257617 (5025 bp), XM_005257618 (5022 bp), ENST00000264657, ENST00000389272, ENST00000404395, ENST00000585517, ENST00000588969
Official symbol Stat3 [Mouse]
Official name signal transducer and activator of transcription 3
Species Mouse (Mus musculus)
Entrez Gene ID 20848
Transcript(s) for this gene NM_011486 (4470 bp), NM_213659 (4520 bp), NM_213660 (4517 bp), XM_001005155 (1260 bp), ENSMUST00000156645, XM_011248846 (4493 bp)
Official symbol Fli1 [Rat]
Official name Fli-1 proto-oncogene, ETS transcription factor
Species Rat (Rattus norvegicus)
Entrez Gene ID 315532
Transcript(s) for this gene NM_001017381 (1871 bp), XM_235979 (1260 bp), XM_008766055 (3125 bp)
Official symbol Rara [Rat]
Official name retinoic acid receptor, alpha
Species Rat (Rattus norvegicus)
Entrez Gene ID 24705
Transcript(s) for this gene NM_031528 (2138 bp), XM_008767945 (2714 bp)
Official symbol MYH11 [Human]
Official name myosin, heavy chain 11, smooth muscle
Species Human (Homo sapiens)
Entrez Gene ID 4629
Transcript(s) for this gene NM_001040113 (6942 bp), NM_001040114 (6903 bp), NM_002474 (6882 bp), NM_022844 (6921 bp), XR_109262 (646 bp), ENST00000576164, ENST00000300036, ENST00000396324, ENST00000452625, ENST00000576790, ENST00000616439
Official symbol Myh11 [Mouse]
Official name myosin, heavy polypeptide 11, smooth muscle
Species Mouse (Mus musculus)
Entrez Gene ID 17880
Transcript(s) for this gene NM_001161775 (6593 bp), NM_013607 (6632 bp), XM_006521843 (6608 bp), XM_006521844 (6647 bp), XM_006521845 (4454 bp)
Official symbol Rara [Mouse]
Official name retinoic acid receptor, alpha
Species Mouse (Mus musculus)
Entrez Gene ID 19401
Transcript(s) for this gene NM_001176528 (3179 bp), NM_001177302 (3384 bp), NM_001177303 (3129 bp), NM_009024 (3251 bp), ENSMUST00000147985, XM_006532591 (3381 bp), XM_006532592 (3166 bp), XM_006532593 (3271 bp), XM_006532594 (3298 bp), XM_006532595 (2621 bp), XM_006532596 (2716 bp), XM_006532597 (2598 bp), XM_006532598 (2674 bp), XM_011248825 (3536 bp)
Official symbol RARA [Human]
Official name retinoic acid receptor, alpha
Species Human (Homo sapiens)
Entrez Gene ID 5914
Transcript(s) for this gene NM_000964 (3301 bp), NM_001024809 (3494 bp), NM_001033603 (2828 bp), NM_001145301 (3413 bp), NM_001145302 (3122 bp), ENST00000582914, XM_005257552 (3284 bp), XM_005257553 (3188 bp), XM_005257554 (3437 bp), XM_005257555 (3240 bp), ENST00000254066, ENST00000394081, ENST00000394086, ENST00000394089, ENST00000425707
Official symbol Gata5 [Mouse]
Official name GATA binding protein 5
Species Mouse (Mus musculus)
Entrez Gene ID 14464
Transcript(s) for this gene NM_008093 (3302 bp), XM_006500554 (3117 bp)
Official symbol GATA5 [Human]
Official name GATA binding protein 5
Species Human (Homo sapiens)
Entrez Gene ID 140628
Transcript(s) for this gene NM_080473 (2595 bp), XM_006723699 (2580 bp), ENST00000252997
Official symbol Gata5 [Rat]
Official name GATA binding protein 5
Species Rat (Rattus norvegicus)
Entrez Gene ID 499951
Transcript(s) for this gene NM_001024316 (2110 bp), XM_008762533 (3552 bp)
Official symbol Sult2a1 [Mouse]
Official name sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
Species Mouse (Mus musculus)
Entrez Gene ID 20859
Transcript(s) for this gene NM_001111296 (1021 bp), XM_001005945 (441 bp), XM_001471625 (979 bp), XM_001477229 (534 bp), XM_905993 (159 bp)
Official symbol Nfe2 [Mouse]
Official name nuclear factor, erythroid derived 2
Species Mouse (Mus musculus)
Entrez Gene ID 18022
Transcript(s) for this gene NM_008685 (1707 bp), NM_001302338 (1896 bp), NM_001302339 (1821 bp), NM_001302340 (1474 bp), NM_001302341 (1509 bp), NM_001302343 (1527 bp), XM_006520569 (1641 bp), XM_006520570 (1816 bp), XM_006520573 (1693 bp)
Official symbol NFE2 [Human]
Official name nuclear factor, erythroid 2
Species Human (Homo sapiens)
Entrez Gene ID 4778
Transcript(s) for this gene NM_001136023 (1698 bp), NM_001261461 (1828 bp), NM_006163 (1697 bp), XM_005268906 (1523 bp), XM_005268907 (1467 bp), ENST00000312156, ENST00000435572, ENST00000540264, ENST00000553070
Official symbol Nfe2 [Rat]
Official name nuclear factor, erythroid 2
Species Rat (Rattus norvegicus)
Entrez Gene ID 366998
Transcript(s) for this gene NM_001012224 (1684 bp), XM_345883 (1212 bp), XM_006242431 (1872 bp), XM_006242432 (1691 bp), XM_006242433 (2317 bp)
Official symbol Nelfcd [Mouse]
Official name negative elongation factor complex member C/D, Th1l
Species Mouse (Mus musculus)
Entrez Gene ID 57314
Transcript(s) for this gene NM_020580 (2279 bp), ENSMUST00000143683, ENSMUST00000139390, XM_006499946 (2318 bp)
Official symbol NELFCD [Human]
Official name negative elongation factor complex member C/D
Species Human (Homo sapiens)
Entrez Gene ID 51497
Transcript(s) for this gene NM_016397 (2162 bp), NM_198976 (2320 bp), ENST00000497935, ENST00000344018, ENST00000602795
Official symbol Fli1 [Mouse]
Official name Friend leukemia integration 1
Species Mouse (Mus musculus)
Entrez Gene ID 14247
Transcript(s) for this gene NM_008026 (3184 bp), XM_006510007 (4318 bp), XM_006510008 (3654 bp), XM_006510009 (3242 bp)
Official symbol FLI1 [Human]
Official name Fli-1 proto-oncogene, ETS transcription factor
Species Human (Homo sapiens)
Entrez Gene ID 2313
Transcript(s) for this gene NM_001167681 (4049 bp), NM_002017 (3995 bp), NM_001271010 (4166 bp), NM_001271012 (3442 bp), ENST00000608055, ENST00000281428, ENST00000344954, ENST00000527786, ENST00000534087
Official symbol Gata4 [Mouse]
Official name GATA binding protein 4
Species Mouse (Mus musculus)
Entrez Gene ID 14463
Transcript(s) for this gene NM_008092 (3405 bp), XM_006518556 (3658 bp), NM_001310610 (3408 bp), XM_011244956 (3411 bp), XM_011244957 (3447 bp)
Official symbol GATA4 [Human]
Official name GATA binding protein 4
Species Human (Homo sapiens)
Entrez Gene ID 2626
Transcript(s) for this gene NM_002052 (3419 bp), ENST00000526021, XM_005272384 (3659 bp), XM_005272385 (3567 bp), XM_005272386 (3726 bp), XM_006716248 (3647 bp), XM_006716249 (2510 bp), ENST00000335135, ENST00000528712, ENST00000532059, ENST00000622443
Official symbol Gata4 [Rat]
Official name GATA binding protein 4
Species Rat (Rattus norvegicus)
Entrez Gene ID 54254
Transcript(s) for this gene NM_144730 (3313 bp), XM_006252189 (2893 bp)
Official symbol Gata2 [Mouse]
Official name GATA binding protein 2
Species Mouse (Mus musculus)
Entrez Gene ID 14461
Transcript(s) for this gene NM_008090 (3258 bp), XM_006505539 (4448 bp)
Official symbol GATA2 [Human]
Official name GATA binding protein 2
Species Human (Homo sapiens)
Entrez Gene ID 2624
Transcript(s) for this gene NM_001145661 (3484 bp), NM_001145662 (3263 bp), NM_032638 (3383 bp), ENST00000489987, ENST00000341105, ENST00000430265, ENST00000487848
Official symbol Gata2 [Rat]
Official name GATA binding protein 2
Species Rat (Rattus norvegicus)
Entrez Gene ID 25159
Transcript(s) for this gene NM_033442 (3411 bp)
Official symbol Jun [Mouse]
Official name jun proto-oncogene
Species Mouse (Mus musculus)
Entrez Gene ID 16476
Transcript(s) for this gene NM_010591 (3187 bp)
Official symbol JUN [Human]
Official name jun proto-oncogene
Species Human (Homo sapiens)
Entrez Gene ID 3725
Transcript(s) for this gene NM_002228 (3338 bp), ENST00000371222
Official symbol Jun [Rat]
Official name jun proto-oncogene
Species Rat (Rattus norvegicus)
Entrez Gene ID 24516
Transcript(s) for this gene NM_021835 (3107 bp)
Official symbol Gata3 [Mouse]
Official name GATA binding protein 3
Species Mouse (Mus musculus)
Entrez Gene ID 14462
Transcript(s) for this gene NM_008091 (3257 bp), ENSMUST00000153509, XM_006497353 (3383 bp), XM_006497354 (3304 bp), XM_006497355 (3330 bp), XM_006497356 (2862 bp)
Official symbol GATA3 [Human]
Official name GATA binding protein 3
Species Human (Homo sapiens)
Entrez Gene ID 2625
Transcript(s) for this gene NM_001002295 (3070 bp), NM_002051 (3067 bp), ENST00000481743, XM_005252442 (2750 bp), XM_005252443 (2618 bp), ENST00000346208, ENST00000379328
Official symbol Gata3 [Rat]
Official name GATA binding protein 3
Species Rat (Rattus norvegicus)
Entrez Gene ID 85471
Transcript(s) for this gene NM_133293 (1335 bp), XM_006254253 (4004 bp), XM_008771893 (3948 bp), XM_008771894 (5538 bp)
Official symbol Gata6 [Mouse]
Official name GATA binding protein 6
Species Mouse (Mus musculus)
Entrez Gene ID 14465
Transcript(s) for this gene NM_010258 (3194 bp), XM_006525652 (3111 bp), XM_006525653 (2996 bp), XM_006525654 (3093 bp), XM_006525655 (3345 bp), XM_006525656 (3679 bp), XM_006525657 (3187 bp)
Official symbol GATA6 [Human]
Official name GATA binding protein 6
Species Human (Homo sapiens)
Entrez Gene ID 2627
Transcript(s) for this gene NM_005257 (3785 bp), XM_005258248 (3704 bp), ENST00000269216, ENST00000581694
Official symbol Myh11 [Rat]
Official name myosin, heavy chain 11, smooth muscle
Species Rat (Rattus norvegicus)
Entrez Gene ID 24582
Transcript(s) for this gene NM_001170600 (6555 bp), XM_001053321 (6348 bp), XM_001053402 (6570 bp), XM_001053471 (6532 bp), XM_001053536 (6555 bp), XM_239866 (3062 bp), XM_573030 (6272 bp), XM_006245842 (6681 bp), XM_006245844 (6676 bp)
Official symbol Zfpm1 [Rat]
Official name zinc finger protein, multitype 1
Species Rat (Rattus norvegicus)
Entrez Gene ID 691504
Transcript(s) for this gene NM_001242627 (3088 bp), XM_001077533 (3055 bp), XM_001077550 (3085 bp), XM_002725423 (3088 bp), XR_007127 (3088 bp), XM_008772646 (6876 bp), XM_008772647 (6864 bp), XM_008772649 (3350 bp)