QuickLyse Miniprep Kit

For fast purification of up to 15 μg sequencing-grade plasmid

  • Rapid one-step lysis procedure
  • Reduced protocol steps and hands-on time
  • Up to 15 μg DNA from 1.5–3 ml culture volumes obtained
  • Ready-to-use plasmid DNA in just 9 minutes

The QuickLyse Miniprep Kit provides a rapid spin-column-based method for isolating sequencing-grade plasmid DNA. Bacterial cell resuspension, lysis, and DNA binding are achieved in a single buffer, enabling fast DNA purification with minimal hands-on time.

产品 Product no. 货号 目录价:
 
 
Show details
    varies
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
产品 货号 目录价:
QuickLyse Miniprep Kit (100)
100 QuickLyse Spin Columns, Reagents, Buffers, Lysis and Collection Tubes (2 ml)
27405
$277.00
加入购物车
QuickLyse Miniprep Kit (250)
250 QuickLyse Spin Columns, Reagents, Buffers, Lysis and Collection Tubes (2 ml)
27406
$590.00
加入购物车

QuickLyse Miniprep Kit适用于分子生物学应用。该产品不适用于疾病的诊断、预防或治疗。


  • Main Image Navi
高品质质粒DNA的产量高。|QuickLyse实验流程。|产量具有可重复性。|自动测序中的读取片段长。|
不同细胞和载体的1.5 ml细菌培养物(LB培养基)中DNA的常规产量和纯度。|QuickLyse采用简单的结合-洗涤-洗脱步骤。|使用QuickLyse Miniprep Kit (QL) 及其他三种商品化的试剂盒(1–3)纯化质粒DNA (pSV-β-半乳糖苷酶)。使用NdeI和EcoRI限制性酶对纯化的质粒DNA进行酶切。反应重复两次。M:分子量标准。Ø:未经酶切的质粒。|使用QuickLyse Miniprep Kit纯化的质粒DNA的一般测序结果。从TOP10细胞中分离pSV-β-半乳糖苷酶质粒,并使用BigDye终止子测序试剂测序。|
性能

The QIAGEN QuickLyse Miniprep Kit uses one-step lysis technology for ultrafast preparation of up to 15 µg plasmid DNA. The quality of the DNA ensures robust performance in applications, such as sequencing, restriction analyses, and cloning (see figure "Reproducible yields").

The QuickLyse Miniprep Kit yields sufficient plasmid DNA for any downstream application, including sequencing (see figure "Long read-lengths in automated sequencing"). Up to 15 µg of high-copy plasmid DNA can be obtained from 1.5 ml of bacterial culture (see figure "High yields of high-quality plasmid DNA"). Isolated plasmid DNA has OD260/280 ratios of 1.7–1.9, indicating that the quality and purity is suitable for all sensitive applications. Sequencing read lengths of over 700 bases are easily achieved.

原理

Unlike most protocols that use a 3-step lysis procedure, the QuickLyse Miniprep Kit combines enzymatic and osmotic processes to lyse bacterial cells in a single, 3-minute step. In addition, QuickLyse technology uses fewer buffers, simplifying handling and saving time: 24 plasmid DNA minipreps can be prepared in less than 22 minutes.

操作流程
Bacterial cells are resuspended and lysed and DNA is bound in a single buffer, enabling fast DNA purification with minimal hands-on time. After a 3-minute lysis step, the clear lysate is applied directly to a QuickLyse Spin Column. Plasmid DNA binds to the column membrane and is eluted after a single wash step (see flowchart "QuickLyse procedure"). The isolated DNA can be used directly in standard applications, including automated sequencing, PCR, restriction analysis, and cloning.
应用

The QuickLyse Miniprep Kit yields sufficient plasmid DNA for any downstream application, including automated sequencing.

特点
参数
应用 Automated sequencing, PCR, restriction analysis, cloning etc.
起始样本体积 1.5 ml culture volume
洗脱体积 50 µl
质粒类型 High-copy, cosmid DNA
处理 Manual (centrifugation)
每次处理样本量;通量 1–24 samples per run
技术 QuickLyse technology
每次运行时间或处理样本数 9 min
产量 3–7 µg

您无权限查看此资源

试剂盒操作手册
1
For purification of sequencing grade plasmid DNA
显示更多
图片
High Yields of High-Quality Plasmid DNA.
高品质质粒DNA的产量高。
不同细胞和载体的1.5 ml细菌培养物(LB培养基)中DNA的常规产量和纯度。
QuickLyse Purification Procedure.
QuickLyse实验流程。
QuickLyse采用简单的结合-洗涤-洗脱步骤。
Reproducible Yields.
产量具有可重复性。
使用QuickLyse Miniprep Kit (QL) 及其他三种商品化的试剂盒(1–3)纯化质粒DNA (pSV-β-半乳糖苷酶)。使用NdeI和EcoRI限制性酶对纯化的质粒DNA进行酶切。反应重复两次。M:分子量标准。Ø:未经酶切的质粒。
Long Read-Lengths in Automated Sequencing.
自动测序中的读取片段长。
使用QuickLyse Miniprep Kit纯化的质粒DNA的一般测序结果。从TOP10细胞中分离pSV-β-半乳糖苷酶质粒,并使用BigDye终止子测序试剂测序。