QIAGEN Clinical Insight (QCI) Analyze

The bioinformatic complement to the GeneReader NGS System
  • Optimized easy-to-use, pre-designed secondary data analysis pipelines
  • Role-based permissions for running, editing, reporting and customizing workflows
  • Fast and reliable globally-recognized algorithms
  • Unique user capability to filter, confirm or reject variants within the review process
  • Concise transparent reporting
QCI Analyze offers all the tools you need to guide your NGS data analysis together with the Actionable Insights Tumor Panel on the GeneReader NGS System. QCI Analyze fully complements the GeneReader, from the setup of sequencing reactions to the automated analysis of sequencing results. The software includes optimized read alignment, variant calling, filtering and integrated visualization so your lab can confirm the analytic validity of your results. After reviewing your quality control (QC) reports and variant data in QCI Analyze, you can continue your interpretation of NGS variants in QCI Interpret.

Please note: The GeneReader NGS System is currently only available with proprietary new sequencing chemistry in the US. Legacy sequencing chemistry is only available ex-US.
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Product Cat. no. List price:
QCI Analyze
Secondary analysis platform including Actionable Insights Tumor Panel Workflow
 188001
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QCIA&I NGS 1200, 1-Node, Standard
QCIA&I NGS 1200, 1-Node, Standard
 830444
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QCIA&I NGS 1200, 1-Node, Sub
QCIA&I NGS 1200, 1-Node, Sub
 830445
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QCIA&I NGS 2400, 3-Node, Standard
QCIA&I NGS 2400, 3-Node, Standard
 830446
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QCIA&I NGS 2400, 3-Node, Sub
QCIA&I NGS 2400, 3-Node, Sub
 830447
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QCIA&I NGS 6000, 5-Node, Standard
QCIA&I NGS 6000, 5-Node, Standard
 830448
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QCIA&I NGS 6000, 5-Node, Sub
QCIA&I NGS 6000, 5-Node, Sub
 830449
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Advanced Testing Solution Starter
Advanced Testing Solution Starter
 832079
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QIAGEN Clinical Insight (QCI) Analyze is for Research Use Only. Not for use in diagnostic procedures

Please note: The GeneReader NGS System is currently only available with proprietary new sequencing chemistry in the US. Legacy sequencing chemistry is only available ex-US.
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Principle
QCI Analyze is a browser-based interface that works with the QIAGEN GeneReader to analyze NGS data and display results for inspection and export. QCI Analyze includes a preinstalled workflow (the GeneRead QIAact Actionable Insights Tumor Panel workflow) that has been generated in the CLC Genomics environment, and which is specific to the GeneRead QIAact Actionable Insights Tumor Panel available in the QIAGEN product portfolio. This kit consists of multiplex PCR primer sets to amplify exonic regions of a thoroughly researched panel of biologically and clinically relevant and disease-focused genes. The associated workflow inputs the corresponding target regions, regions of interest and variants of interest.

Several users can access the data through their browsers and there is no need to install software on each computer accessing the NGS data. Analyses can be controlled and streamlined and all user activity can be tracked on a per sample basis. Behind QCI Analyze, a CLC Genomics Server runs the analyses, stores data and handles various processes such as queuing.

QCI Analyze plugins enable the automatic analysis of sequencing data as soon as it is generated by the GeneReader and provide connectivity to QCI Interpret.
Procedure
QCI Analyze workflow

The QCI Analyze workflow provides streamlined and standardized analysis of the next-generation sequencing (NGS) data generated by the GeneReader. The workflow is automated, ensuring greater standardization and more accurate results. The QCI Analyze process includes:

  • Quality control to ensure that the quality of the data generated and analyzed meets specified criteria
  • Optimized re-sequencing workflows to convert the GeneReader generated sequencing files (FASTQ files) into interpretable results
  • Visualization to enable review and confirmation of sequencing variants

The workflow creates a web accessible interactive report that includes:

  • Summary results
  • Gene and variant level statistics
  • Detailed QC
  • Audit trail
  • A track list for a visual display of the variants

Administrator options enable lab-specific criteria for variant reporting, thus locked down workflows for the general user.
Applications
QCI Analyze is a secondary analysis software intended for the analysis of data generated by automated sequencers like the GeneReader. 
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