QIAamp MinElute Virus Vacuum Kit
For simultaneous purification of viral DNA and RNA from plasma, serum, and cell-free body fluids
- Rapid purification of high-quality viral DNA and RNA
- No organic extraction or alcohol precipitation
- Consistent, high yields
- Removal of contaminants and inhibitors
The QIAamp MinElute Virus Vacuum Kit simplifies purification of viral DNA and RNA with fast vacuum procedures. The QIAamp MinElute Virus Vacuum Kit can be used with starting sample volumes up to 0.5 ml. The kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 μl.
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QIAamp MinElute Virus Vacuum Kit (50)
For 50 minipreps: 50 QIAamp MinElute Columns, QIAGEN Protease, carrier RNA, buffers, Extension Tubes (3 ml), Collection Tubes (1.5 ml)
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57714
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The QIAamp MinElute Virus Vacuum Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAamp MinElute Virus Vacuum procedure.|High sensitivity in PCR.|High sensitivity in RT-PCR.|
|The percentage of positive samples at low virus titers is shown. Nucleic acids from a DNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subjected to PCR. For the spin procedure, 95% probit values for 40 samples were 27.25 IU/ml and for the vacuum procedure, they were 9.30 IU/ml. |The percentage of positive samples at low virus titers is shown. Nucleic acids from an RNA virus were purified from plasma using either the QIAamp MinElute Spin or Vacuum Kit and subject to RT-PCR. For the spin procedure, 95% probit values for 32 samples were 22.84 IU/ml and for the vacuum procedure, they were 9.46 IU/ml.|
Performance
Purified viral nucleic acids are free of proteins, nucleases, and other impurities, and suitable for use in sensitive downstream applications such as PCR and RT-PCR (see figures "High sensitivity in PCR" and "High sensitivity in RT-PCR").
Viral nucleic acids purified using the QIAamp MinElute Virus Vacuum Kit can be used in a wide range of downstream applications, including:
- PCR and quantitative real-time PCR
- Infectious disease research
Principle
The QIAamp MinElute Virus Vacuum Kit simplifies isolation of viral RNA and DNA from plasma, serum, and cell-free body fluids with a fast vacuum procedure. No phenol–chloroform extraction is required. Nucleic acids bind specifically to the QIAamp MinElute silica-gel membrane while contaminants pass through. PCR inhibitors, such as divalent cations and proteins, are completely removed in two efficient wash steps, leaving pure nucleic acids to be eluted in either water or a buffer provided with the kit. QIAamp MinElute technology yields viral RNA and DNA from plasma, serum, and cell-free body fluids that are ready to use in PCR and blotting procedures.
The QIAamp MinElute Virus Vacuum Kit combines the selective binding properties of a silica-based membrane with flexible elution volumes of between 20 and 150 µl. QIAamp sample preparation technology is fully licensed.
Procedure
Optimized buffers lyse samples, stabilize nucleic acids, and enhance selective DNA adsorption to the QIAamp MinElute membrane (see flowchart " Procedure"). Alcohol is added and lysates loaded onto the QIAamp MinElute spin column. Wash buffers are used to remove impurities and viral nucleic acids are eluted in Buffer AVE, ready for use in amplification reactions or storage at –20ºC. Purified nucleic acids are free of proteins, nucleases, and other impurities.
Applications
The QIAamp MinElute Virus Vacuum Kit uses well-established technology for simultaneous purification of viral RNA and DNA. Fresh or frozen plasma, serum, and other cell-free body fluids (500 µl) can be processed in less than an hour.
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Feature
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Specifications
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Applications
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PCR, real-time PCR
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Elution volume
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20–150 µl
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Format
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MinElute columns
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Main sample type
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Serum, plasma
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Processing
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Manual (vacuum)
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Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
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Viral DNA, viral RNA
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Sample amount
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500 µl
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Technology
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Silica technology
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Time per run or per prep
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<1 hour
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Yield
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Varies
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I received one of the kits in the list below containing the MinElute columns, however they were left out for a while and not stored at 2-8°C upon receipt. Can I still use them? AllPrep DNA/RNA Micro, EpiTect Fast DNA Bisulfite, EpiTect Fast FFPE Bisulfite, EpiTect Fast LyseAll Bisulfite, EpiTect Plus DNA Bisulfite, EpiTect Plus FFPE Bisulfite, EpiTect Plus LyseAll Bisulfite, exoRNeasy Serum/plasma Maxi, exoRNeasy Serum/Plasma Midi, GeneRead DNA FFPE, GeneRead rRNA Depletion, GeneRead Size Selection, MinElute Gel Extraction, MinElute PCR Purification, MinElute Reaction Cleanup, miRNeasy FFPE, miRNeasy Micro, miRNeasy Serum/Plasma, QIAamp DNA FFPE, QIAamp DNA Investigator, QIAamp DNA Micro, QIAamp MinElute Media, QIAamp MinElute Virus Spin, QIAamp MinElute Virus Vacuum, RNeasy FFPE, RNeasy Micro, RNeasy Plus Micro
FAQ ID - 3560
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For simultaneous purification of viral RNA and DNA from plasma, serum, and cell-free body fluids
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This procedure has been adapted by customers and is for purification of viral RNA and DNA from plasma, serum, and cell-free body fluids using the QIAamp MinElute Virus Vacuum Kit.
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