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RT2 qPCR Primer Assay (200)
RT2 qPCR Primer Assay
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330001
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RT2 qPCR Primer Assays are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Principle
RT² qPCR Primer Assays use
SYBR® Green-based quantitative real-time PCR technology to provide a sensitive and reliable tool for gene expression analysis.
Each assay utilizes a proprietary and experimentally verified algorithm for the design of gene-specific qPCR primers with uniform PCR efficiency and amplification conditions. Each lot of every assay is further wet-bench tested for real-time PCR performance for specificity and amplification efficiency. Amplification of a single product of the correct size with high PCR efficiency (>90%) is guaranteed when the assays are used with RT² SYBR® Green qPCR Mastermixes. The uniform PCR efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an accurate and scalable solution for multiple gene expression analyses.
Applications
RT2 qPCR Primer Assays are highly suited for applications including validation of microarray-derived gene expression data, confirming gene expression knockdown by RNAi, identifying and confirming disease-associated biomarkers, and monitoring phenotypic changes related to gene expression. Multiple RT2 qPCR Primer Assays can also be used to examine a focused panel of genes.