en-IE
Configure
|
Product
|
Product no.
|
Cat. no.
|
List price:
|
|
|
This product has been discontinued.
Instead, we recommend
|
|
|
|
|
varies
|
select
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
|
| Product |
Cat. no. |
List price: |
|
|
Product
|
Product no.
|
Cat. no.
|
List price:
|
|
Configure
|
Product
|
Product no.
|
Cat. no.
|
List price:
|
|
|
This product has been discontinued.
Instead, we recommend
|
|
|
|
|
varies
|
select
Can't order online?
To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number.
|
| Product |
Cat. no. |
List price: |
|
|
Product
|
Product no. |
Cat. no.
|
List price:
|
|
RT2 lncRNA PreAMP Primer Mixes are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Principle
Each RT2 lncRNA PreAMP Primer Mix is specific for one RT2 lncRNA PCR Array. During the amplification step, the RT2 lncRNA PreAMP Primer Mix enables amplification of cDNA specific for the genes targeted by the RT2 lncRNA PCR Array. A cDNA synthesis reaction from 1–100 ng total RNA from fresh/frozen samples or 100 ng–1 µg total RNA from FFPE samples provides sufficient cDNA for amplification by 4 different RT2 lncRNA PreAMP Primer Mixes, allowing gene expression analysis of up to 4 different pathways. Following preamplification, the Side Reaction Reducer eliminates residual primers. Preamplified cDNA is then ready for PCR array analysis using the appropriate RT2 lncRNA PCR Array.
Procedure
Preamplification is a simple PCR reaction. Following cDNA synthesis, preamplification mix is prepared by mixing RT2 lncRNA PreAMP Primer Mix with RT2 PreAMP Mastermix, then added to cDNA template in a 96-well plate. The plate is placed in a real-time cycler, and follows a 12-cycle program detailed in the handbook. Side Reaction Reducer is added following cycling, then heat-inactivated.The amplified cDNA is stored on ice or at –20°C until use.
Applications
RT2 lncRNA PreAMP Primer Mixes are highly suited for use with small or fragmented RNA from laser-capture microdissection, fine needle aspiration biopsy, fluorescence-activated cell sorting, or FFPE samples, followed by lncRNA gene expression analysis with RT2 lncRNA PCR Arrays.
