DNeasy Blood & Tissue Kits
For spin-column or 96-well purification of total DNA from animal blood and tissues and from cells, yeast, bacteria, or viruses
- Standardized method for a variety of sample types
- High yields even from specialized samples
- High-quality DNA
- Optimized protocols for a range of starting materials
- Spin-column and 96-well high-throughput formats
DNeasy Blood & Tissue Kits provide fast and easy silica-based DNA purification in convenient spin-column and 96-well-plate formats. Most samples can be directly lysed with proteinase K, eliminating the need for mechanical disruption and reducing hands-on time. Optimized protocols for specific sample types provide reproducible purification of high-quality DNA for life science, genotyping, and veterinary pathogen research applications. Purification of DNA using the DNeasy Blood & Tissue Kit can be automated on the QIAcube.
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DNeasy 96 Blood & Tissue Kit (4)
For 4 x 96 DNA minipreps: 4 DNeasy 96 Plates, Proteinase K, Buffers, S-Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Elution Microtubes RS, Caps, 96-Well Plate Registers
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69581
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DNeasy 96 Blood & Tissue Kit (12)
For 12 x 96 DNA minipreps: 12 DNeasy 96 Plates, Proteinase K, Buffers, S-Blocks, AirPore Tape Sheets, Collection Microtubes (1.2 ml), Elution Microtubes RS, Caps, 96-Well Plate Registers
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69582
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DNeasy Blood & Tissue Kit (50)
50 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)
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69504
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DNeasy Blood & Tissue Kit (250)
250 DNeasy Mini Spin Columns, Proteinase K, Buffers, Collection Tubes (2 ml)
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69506
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The DNeasy Blood & Tissue Kits is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
DNA yields.|DNeasy Mini and 96 procedures.|Efficient 16plex PCR.|High-quality DNA.|Genotyping of horses.|High-throughput purification.|Real-time PCR.|Genotyping of pigs.|
DNA was purified from 100 µl whole blood using the DNeasy Blood & Tissue Kit. Goat: DNA was purified from 50 µl goat whole blood. Using more than 50 µl goat blood gave no significant increase in DNA yield. Chicken: DNA was purified from 5 µl chicken whole blood. Bird blood contains nucleated erythrocytes, giving higher DNA yields than mammalian blood.||Genomic DNA was purified from human K562 cells using the DNeasy Blood & Tissue Kit. Multiplex PCRs were performed in duplicate using the QIAGEN Multiplex PCR Kit with the indicated amount of DNA and 16 pairs of primers. M: 100 bp ladder.|DNA was purified from the indicated animal tissues using the DNeasy Blood & Tissue Kit and analyzed by agarose gel electrophoresis. For each sample, 0.5 µg DNA was analyzed undigested (left) or 1 µg DNA was digested with EcoRI (right). M: lambda-HindIII.|DNA was purified from hair of 3 brown horses and analyzed for coat-color genotypes using the MSH and ASIP PCR systems. [A] MSH PCR products were digested with TaqI. Horses 1 and 3 were identified as Ee heterozygotes, carrying the recessive e allele for red coat color. [B] Analysis of ASIP PCR products identify horses 1 and 2 as Aa heterozygotes, carrying the recessive a allele for black coat color. +: Positive Ee aa control DNA from a black horse. -: Negative PCR control. M: markers.|DNA was purified from 24 mouse tails and from 24 rat tails using the DNeasy 96 Blood & Tissue Kit. DNA was analyzed on a 1% agarose gel. M: lambda-HindIII.|Borrelia sp. is a pathogen carried by ticks that causes Lyme disease in humans and other mammals. DNA was purified from ticks using the DNeasy Blood & Tissue Kit. Borrelia DNA was detected in real-time PCR using the artus Borrelia LC PCR Kit on the LightCycler system. The lowest positive curve corresponds to 5 copies per PCR.|DNA was purified from pig ear punches using the DNeasy Blood & Tissue Kit and amplified using the Animaltype Pig PCR Amplification Kit from Biotype AG and QIAGEN HotStarTaq DNA Polymerase. The electropherogram shows the genetic fingerprint for 12 polymorphic tetranucleotide microsatellites and one marker for gender determination (SBH23).
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Performance
The efficient DNeasy Blood & Tissue procedure enables high yields of total DNA from animal blood and tissue samples (see table "Typical DNA yields from animal tissues using DNeasy Blood & Tissue Kits" and figure "DNA yields"). Optimized protocols ensure high yields from nonstandard samples, such as animal hair (see figure "Genotyping of horses"), as well as cultured cells, fixed tissues, or Gram-positive and -negative bacteria. Specialized online protocols are available for DNA purification from yeast, insects, hair, and other sample types.
| Mammalian blood |
100 µl |
3–6 |
| Bird blood |
5 µl |
9–40 |
| HeLa cells |
2 x 106 |
15–25 |
| Liver |
25 mg |
10–30 |
| Brain |
25 mg |
15–30 |
| Kidney |
25 mg |
15–30 |
| Spleen |
10 mg |
5–30 |
| Mouse tail |
1.2 cm (tip) |
10–25 |
| Rat tail |
0.6 cm (tip) |
20–40 |
| Pig ear |
25 mg |
10–30 |
| Horse hair |
10 hairs |
2–4 |
| Fish fin |
20 mg |
10–20 |
| Fish spawn (mackerel) |
10 mg |
5–10 |
DNeasy Blood & Tissue Kits simplify purification of DNA from a wide range of sample types, including animal species commonly encountered in life science, veterinary, and genotyping applications (see figure "High-quality DNA"). Purified DNA is free from PCR inhibitors, enabling sensitive detection in standard, multiplex (see figure "Efficient 16plex PCR"), and real-time PCR (see figure "Real-time PCR"). DNeasy Blood & Tissue Kits provide reliable results — from laboratory analysis of transgenic mice (see figure "High-throughput purification") to livestock breeding programs (see figure "Genotyping of pigs") and pedigree genotyping. Routine testing applications can be easily scaled up using the DNeasy 96 Blood & Tissue Kit (see figure "High-throughput purification").
Principle
DNeasy Blood & Tissue Kits are designed for rapid purification of total DNA (e.g., genomic, mitochondrial, and pathogen) from a variety of sample sources including fresh or frozen animal tissues and cells, blood, or bacteria.
The DNeasy membrane combines the binding properties of a silica-based membrane with simple microspin technology or with the QIAGEN 96-Well-Plate Centrifugation System. DNA adsorbs to the DNeasy membrane in the presence of high concentrations of chaotropic salt, which remove water from hydrated molecules in solution. Buffer conditions in DNeasy Blood & Tissue procedures are designed to enable specific adsorption of DNA to the silica membrane and optimal removal of contaminants and enzyme inhibitors.
Purification requires no phenol or chloroform extraction or alcohol precipitation, and involves minimal handling. This makes DNeasy Blood & Tissue Kits highly suited for simultaneous processing of multiple samples. For higher-throughput applications, the DNeasy 96 Blood & Tissue Kit enables simultaneous processing of 96 or 192 samples.
Procedure
Reliable silica-membrane technology, in convenient spin-column or 96-well formats, ensures fast and reproducible DNA purification, eliminating the need for organic extraction and alcohol precipitation (see flowchart "DNeasy Mini and 96 procedures"). Samples are first lysed using proteinase K. Buffering conditions are adjusted to provide optimal DNA-binding conditions and the lysate is loaded onto the DNeasy Mini spin column or the DNeasy 96 plate. During centrifugation, DNA is selectively bound to the DNeasy membrane as contaminants pass through. Remaining contaminants and enzyme inhibitors are removed in two efficient wash steps and DNA is then eluted in water or buffer, ready for use.
DNeasy Mini spin columns are prepackaged in collection tubes and individually sealed, providing convenience and safety. The purification procedure using DNeasy Mini spin columns can be automated on the QIAcube. The DNeasy 96 Blood & Tissue Kit provides high-throughput processing in a 96-well format using the QIAGEN 96-Well-Plate Centrifugation System.
Applications
DNeasy Blood & Tissue Kits provide high-quality DNA, ready to use in all downstream assays, including applications in:
Life science research
Livestock breeding
Pedigree genotyping
Veterinary pathogen research
Routine applied testing
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Feature
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Specifications
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Applications
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PCR, real-time PCR, genotyping
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Elution volume
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100–200 µl
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Format
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96-well plate, spin column
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Main sample type
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Blood, tissue
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Processing
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Manual
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Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein
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DNA
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Sample amount
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100 µl/25 mg
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Technology
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Silica technology
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Time per run or per prep
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20 minutes – 1 hour
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Yield
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6 µg/30 µg
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For purification of total DNA from animal blood, animal tissue, rodent tails, ear punches, cultured cells, fixed tissue, bacteria, insects
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Show details
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This protocol is designed for purification of DNA from up to 5 x 107 yeast cells.
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Show details
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This protocol is designed for purification of DNA from up to 50 mg of insects, such as drosophila.
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Show details
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This protocol is designed for purification of DNA from a 200 μl crude lysate.
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Show details
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