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PyroMark Q24 CpG LINE-1 (4 x 24)
Methylation detection assay: PCR primers and sequencing primer for quantification of global methylation level in transposable elements
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970042
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PyroMark Q24 CpG MGMT (4 x 24)
Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +17 to +39 of exon 1 of the MGMT gene
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970032
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PyroMark Q24 CpG MLH1 (4 x 24)
Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region –209 to –181 from the transcription start site in the MLH1 gene
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970022
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PyroMark Q24 CpG p16 (4 x 24)
Methylation detection assay: PCR primers and sequencing primer for quantification of methylation level in region +148 to +182 in exon 1 of the gene
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970012
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PyroMark HFE (96)
For detection of variants in the HFE gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)
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972442
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PyroMark MTHFR (96)
For detection of variants in the MTHFR gene (2 x 96 reactions on PyroMark Q96 MD, 1 x 96 reactions on PyroMark Q96 ID, 8 x 24 reactions on PyroMark Q24)
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972432
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PyroMark Q24 Tests are intended for molecular biology applications. These products are not intended for the diagnosis, prevention, or treatment of a disease.
Principle
PyroMark Q24 CpG LINE-1, PyroMark Q24 CpG MGMT, PyroMark Q24 CpG MLH1, and PyroMark Q24 CpG p16 enable highly reliable methylation results. In addition, a built-in control for bisulfite treatment improves accuracy and reproducibility of the results.
Procedure
Prior to Pyrosequencing, a biotinylated PCR product is generated. Biotinylated PCR products are bound to streptavidin-coated Sepharose beads. The beads are then captured with the vacuum tool on the PyroMark Vacuum Workstation, and throroughly washed, generating single-stranded DNA suitable for sequencing. PyroMark Q24 Tests are predesigned assays for methylation analysis by Pyrosequencing. Each test includes the primers necessary for generating the biotinylated PCR product, as well as the primer for the Pyrosequencing reaction.
Applications
PyroMark Q24 Tests enable highly accurate, quantitative methylation and mutation (or SNP) analysis applicable in several fields of research, including cancer and epigenetics.