REPLI-g Single Cell Kit
|
Single-cell whole genome amplification
|
Single cells,
1–1000 cells, tissue, purified gDNA (1–10 ng)
|
Whole genome amplified DNA for analysis by cGH array, microarray, qPCR or NGS with the highest sensitivity
|
40 µg
|
8–16 h
|
15 min
|
REPLI-g WTA Single Cell Kit
|
Single-cell whole transcriptome amplification
|
Single cells (mammalian),
1–1000 cells, purified RNA (10 pg – 100 ng)
|
Whole transcriptome amplified cDNA for subsequent analysis by microarray, qPCR or NGS with the highest sensitivity
|
20 µg cDNA
|
4 h
|
20 min
|
REPLI-g Cell WGA & WTA Kit
|
Parrallel whole genome and whole transcriptome amplification starting with a few cells
|
25–1000 mammalian cells
|
Whole genome amplified DNA and whole transcriptome amplified cDNA
|
Approx. 20 µg cDNA, approx. 20 µg DNA
|
4 h
|
20 min
|
REPLI-g Mini Kit
|
Small-sample whole genome amplification
|
>10 ng gDNA, 0.5 µl blood or cells (600 cells/µl)
|
Whole genome amplified DNA for subsequent analysis by microarray, cGH array, qPCR, Sanger sequencing or NGS
|
10–40 µg
|
10–16 h
|
15 min
|
REPLI-g UltraFast Mini Kit
|
Small-sample whole genome amplification, with a focus on short time-to-result
|
7–10 µg
|
1.5 h
|
15 min
|
REPLI-g Midi Kit
|
Small-sample whole genome amplification, with a focus on high yield
|
40 µg
|
8–16 h
|
15 min
|
REPLI-g Screening Kit
|
Small-sample whole genome amplification, with a focus on high sample throughput
|
8 µg
|
12–16 h
|
15 min
|
REPLI-g FFPE Kit
|
Whole genome amplification of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue
|
FFPE tissue section (1 cm diameter, 10–10 µm thick); 100 ng gDNA from FFPE tissue
|
Standard yield: ≤10; high-yield: ≤40
|
Standard yield: 4 h;
high yield: 10 h
|
40 min
|
REPLI-g Mitochondrial DNA Kit
|
Whole genome amplification of mitochondrial DNA
|
>1 ng purified gDNA
|
8 µg
|
8 h
|
15 min
|