Are QIAprep and QIAquick Spin columns interchangeable?
FAQ ID -311
No. While columns from the
QIAprep Spin Miniprep Kit and the
QIAquick PCR Purification- and
Gel Extraction Kits are based on silica-gel-membrane technology, each is designed to work optimally within its own kit format. In addition, the binding capacity and DNA recovery size cut-offs of the QIAprep and QIAquick Spin columns are different. QIAprep Spin columns bind up to 20 ug of plasmid DNA up to 50 kb in length, while the QIAquick Spin columns bind up to 10 ug of DNA with a maximum fragment size of 10 kb.