RNA was isolated from fresh rat kidney samples after 0, 5, 10, 15, 30, and 60 minutes, using either standard procedures (Unstabilized) or RNeasy Protect Kits (Stabilized). The RNA isolated was analyzed by agarose gel electrophoresis and expression of GAPDH was examined using northern blot analysis. M: markers.
Once a biological sample is harvested, its RNA becomes extremely unstable. Gene induction or down-regulation triggered by sample manipulation must be prevented to enable accurate gene expression analysis.
For purification of total RNA from formalin-fixed, paraffin-embedded tissue sections.