RNeasy 96 Universal Tissue Kit
For 96-well purification of total RNA from any tissue
The RNeasy 96 Universal Tissue Kit enables total RNA purification in 96-well format from any tissue. Tissues are efficiently lysed and homogenized in QIAzol Lysis Reagent, and high-quality RNA is purified using silica-membrane RNeasy 96 plates. Manual purification is carried out using the QIAGEN 96-Well-Plate Centrifugation System and, optionally, the QIAvac 96 vacuum manifold. Automated purification using the related RNeasy 96 Universal Tissue 8000 Kit is performed on the BioRobot Universal System. Tissue samples can be conveniently stabilized using Allprotect Tissue Reagent, and efficiently disrupted using the TissueLyser II. Nonfatty tissues can be stabilized using the RNAlater RNA Stabilization Reagent.
Compared with standard silica-membrane procedures, the RNeasy 96 Universal Tissue Kit provides higher yields of total RNA for all tissue types (see table).
High-quality RNA can be isolated from tissues preserved either by freezing or by stabilization with RNAlater at room temperature (see figure "High-quality total RNA from rat liver and muscle tissue"). Intact RNA purified from difficult-to-lyse fibrous and fatty tissues is suitable for downstream applications (see figures "High-quality RNA from skin / adipose / liver / intestine tissue" and "Real-time analysis of high-quality RNA from rat brain").
The RNeasy 96 Universal Tissue procedure enables high-throughput purification of RNA from any animal or human tissue sample. Compared with standard silica-membrane procedures, the RNeasy 96 Universal Tissue Kit provides higher yields of total RNA for all tissue types. The RNeasy procedure enriches for RNA >200 bases long, providing a yield of total RNA that does not include 5.8S rRNA, tRNA, and other low-molecular weight RNAs, which make up 15–20% of total cellular RNA.
The optimized RNeasy 96 Universal Tissue procedure integrates TissueLyser/QIAzol processing with RNeasy 96 purification in an easy-to-follow protocol (see flowchart "RNeasy 96 Universal Tissue procedure"). Efficient high-throughput disruption using the TissueLyser II provides parallel disruption and homogenization, with lysis of up to 192 samples in QIAzol Lysis Reagent. Protocol steps are performed manually using the QIAGEN 96-Well-Plate Centrifugation System, with optional use of the QIAvac 96 vacuum manifold. For automated processing, see the RNeasy 96 Universal Tissue 8000 Kit.
The combination of QIAzol and RNeasy technologies results in highly pure RNA without phenol carryover. RNA purified using the RNeasy 96 Universal Tissue Kit is suitable for all downstream applications, including real-time RT-PCR and array analysis.
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