QIAamp UCP DNA Micro Kit

For isolation of ultraclean genomic DNA from small amounts of blood, swabs, cells or tissue

Features

  • High yields from small amounts of blood, cells, swabs and tissue
  • Ultraclean production (UCP) components and strict QC processes minimizing the risk of foreign contamination
  • Procedure automatable on QIAcube
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QIAamp UCP DNA Micro Kit

Cat. No. / ID: 56204

For 50 preps: QIAamp UCP MinElute spin columns, QIAGEN Proteinase K, Buffers
The QIAamp UCP DNA Micro Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details

The QIAamp UCP DNA Micro Kit enables easy DNA purification from small amounts of blood, tissues, cells and swabs. An integral ultraclean treatment removes residual DNA contamination from the QIAamp UCP MinElute column. The procedure can be automated on QIAcube.

Performance

The QIAamp UCP DNA Micro Kit is suitable for a wide range of sample materials, such as small volumes of blood, animal and human cells, swabs and small tissue samples.

Special ultraclean treatment removes residual DNA contamination from the QIAamp UCP MinElute column, preventing DNA contamination from influencing downstream results (see figure “ Minimal background compared with other suppliers”). Eluted nucleic acids are ready for use in downstream applications including next-generation sequencing (see figure “ Performance of ultraclean DNA in NGS”). 

See figures

Principle

The QIAamp UCP DNA Micro Kit is designed for purification of ultraclean genomic, mitochondrial and microbial DNA from small sample volumes. The kit combines the selective binding properties of a silica-based membrane with elution volumes of between 20 and 100 µl.

DNA yield and purity obtained with the QIAamp UCP DNA Micro Kit are comparable to known methods, but with very little background of foreign DNA (see figure “ Comparable yields”). 

See figures

Procedure

A simple workflow allows the purification of ultraclean DNA from whole blood, cells, swabs or tissue (see flowchart “ QIAamp UCP DNA Micro procedure”).

Samples are lysed under highly denaturing conditions at elevated temperatures in the presence of proteinase K and Buffer AUT. DNA is then bound to the QIAamp UCP MinElute column membrane and two wash steps efficiently wash away contaminants. DNA is eluted from the QIAamp UCP MinElute column using a small volume of Buffer AUE or ultraclean PCR water. Since the elution volume is small, the eluted DNA will be concentrated. 

 

See figures

Applications

DNA purified using the QIAamp UCP DNA Micro Kit is suitable for use in NGS and other downstream applications requiring the use of ultraclean DNA.

Supporting data and figures

Specifications

FeaturesSpecifications
Purification of total RNA, miRNA, poly A+ mRNA, DNA or proteinGenomic DNA and viral nucleic acids

Resources

FAQ

Are there important considerations for plasma generation and urine handling?

It is strongly advised to follow the recommendations for preparing sample material provided in the corresponding Protocol Sheet to ensure reliable results.

Plasma: It is recommended to perform plasma separation immediately after blood collection when using EDTA or citrate as anticoagulant to prevent the release of genomic DNA into the plasma fraction.

Urine: Because circulating cell-free DNA in non-stabilized urine samples is rapidly degraded after sample collection due to high nuclease activity, eluates may contain no DNA or exhibit low DNA concentration. Therefore, it is recommended to stabilize urine samples. Even when using stabilized urine, it is recommended to perform a centrifugation step immediately after stabilization to prevent the release of genomic DNA from cells. Alternatively, non-stabilized urine samples can be processed immediately after collection and centrifugation using ATL-pretreatment and automated DNA extraction as described in the corresponding Protocol Sheet.

FAQ ID - 3699