The miRCURY LNA miRNA miRNome PCR Panels enable exceptionally sensitive, high-throughput expression profiling from minimal amounts of starting material. The combination of a universal cDNA synthesis reaction and ready-to-use PCR panels provides fast and easy miRNA profiling. The panels are provided in 384-well plates that contain dried-down primers for one reaction per well and simply require addition of cDNA and miRCURY LNA SYBR Green Master Mix prior to real-time PCR amplification.
The LNA-enhanced, miRNA-specific PCR primers offer exceptional sensitivity and extremely low background, enabling accurate quantitation of very low miRNA levels. Plus, high specificity allows discrimination between closely related miRNA sequences, with a quick and easy protocol that gives you results in just 3 hours.
Two panels covering 752 human miRNAs and two panels covering 752 mouse and rat miRNAs are available. Panel I for human and mouse/rat contains high-priority miRNA primer sets. These miRNAs are generally more highly expressed, more likely to be differentially expressed in disease or more often cited in the literature. Panel II contains primer sets for the most important miRNAs not available on Panel I. To view the list of primer sets in each panel, download the plate layout files.
The miRNome PCR Panels also include 6 potential reference gene primer sets – three small RNA reference genes (U6, SNORD38B and SNORD49A for human and U6 snRNA, RNU5G, RNU1A for mouse and rat) and three miRNA reference genes (miR-103-3p, miR-191-5p and hsa-miR-423-5p or mmu-miR-423-3p) – all found on Panel I. Panel I also contains qPCR assays for the 5 synthetic RNAs in the RNA Spike-in Kit (cel-miR-39-3p, UniSp2, UniSp4, UniSp5 and UniSp6) that may be used for monitoring the cDNA synthesis or RNA purification. Finally, each panel contains an inter-plate calibrator in triplicate and an empty negative control.
The updated miRCURY LNA miRNA miRNome PCR Panels include 54 updated assays on Panel I and 33 updated assays on Panel II. The assays target the same miRNAs as v3 panels. In addition, the two spike-in primer sets UniSp5 and UniSp6 have been updated for improved performance. Their respective target templates will be the same.